Cell titer glow kit

Manufactured by Promega

The CellTiter-Glo® Luminescent Cell Viability Assay is a method of determining the number of viable cells in culture based on quantitation of the ATP present, which signals the presence of metabolically active cells. The assay involves adding a single reagent directly to cells, and measuring the luminescent signal which is proportional to the amount of ATP present.

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Cell Titer Glow (37 citations)

2 protocols using cell titer glow kit

Nanoparticle-induced Cardiac ATP Depletion

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ATP was measured using the Cell Titer Glow kit (Promega) in isolated ventricle myocytes (10⁴ cells) after 24 h exposure to nanoSiO₂ at LD50, and in ventricle tissue after perfusion of nanoSiO₂ in the isolated heart. Cells or N₂ frozen-grinded tissue in p96 well format were lysed according to the manufacturer instruction to record the luminescence derived from the ATP-dependent luciferase activity, using a microplate luminometer. ATP was quantified using a standard curve and normalized to the total protein in the sample.

Lozano O., Silva-Platas C., Chapoy-Villanueva H., Pérez B.E., Lees J.G., Ramachandra C.J., Contreras-Torres F.F., Lázaro-Alfaro A., Luna-Figueroa E., Bernal-Ramírez J., Gordillo-Galeano A., Benitez A., Oropeza-Almazán Y., Castillo E.C., Koh P.L., Hausenloy D.J., Lim S.Y, & García-Rivas G. (2020). Amorphous SiO2 nanoparticles promote cardiac dysfunction via the opening of the mitochondrial permeability transition pore in rat heart and human cardiomyocytes. Particle and Fibre Toxicology, 17, 15.

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Cellular ATP and Lactate Assay

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2 × 10⁵ cells were grown in 96-well plates. Cellular ATP was measured by ATP using Cell Titer Glow kit (Promega), and total lactate was measured using Lactate Colorimetric/Fluorometric (BioVision) Assay Kit and was measured in triplicate following the protocol provided.

Tlili M., Acevedo H., Descoteaux A., Germain M, & Heinonen K.M. (2022). Cell-intrinsic Wnt4 ligand regulates mitochondrial oxidative phosphorylation in macrophages. The Journal of Biological Chemistry, 298(8), 102193.

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