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Penicillin and streptomycin

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Penicillin and streptomycin are broad-spectrum antibiotics commonly used in laboratory settings. Penicillin is a beta-lactam antibiotic that disrupts bacterial cell wall synthesis, while streptomycin is an aminoglycoside antibiotic that inhibits protein synthesis in bacteria. These antibiotics are often used together to provide a wide range of antimicrobial activity in various laboratory applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by GenDEPOT (2 mentions) 1640 medium, by GenDEPOT (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Mkn45, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Nci n87, by Korean Cell Line Bank (1 mentions) Mkn74, by American Type Culture Collection (1 mentions) Snu 638, by Korean Cell Line Bank (1 mentions)

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Penicillin/streptomycin (38 citations) Penicillin (16 citations) Streptomycin (15 citations)

3 protocols using penicillin and streptomycin

1

Maintenance of Human Cell Lines

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The human cancer cell lines HT29 (colon cancer), AGS (gastric cancer), A549 (lung cancer), and CWR22Rv-1 (prostate cancer) were maintained in Roswell Park Memorial Institute (RPMI) 1640 medium (Gen Depot, USA) supplemented with 10% fetal bovine serum (FBS) (Gen Depot, USA) and 1% penicillin and streptomycin (RPMI complete medium) (Gen Depot, USA). HaCaT (human keratinocyte), NIH 3T3 (mouse embryonic fibroblast cells), HEK293T (human embryonic kidney) cells, RIE (rat intestinal epithelial) cells, and Madin-Darby canine kidney (MDCK) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Gen Depot, USA) supplemented with 10% FBS and 1% penicillin and streptomycin. Cells were cultured in 5% CO2 in a humidified atmosphere at 37°C. Cell lines were purchased from the Korean Cell Line Bank (http://cellbank.snu.ac.kr), Korea.
Nguyen T.T., Yoon S., Yang Y., Lee H.B., Oh S., Jeong M.H., Kim J.J., Yee S.T., Crişan F., Moon C., Lee K.Y., Kim K.K., Hur J.S, & Kim H. (2014). Lichen Secondary Metabolites in Flavocetraria cucullata Exhibit Anti-Cancer Effects on Human Cancer Cells through the Induction of Apoptosis and Suppression of Tumorigenic Potentials. PLoS ONE, 9(10), e111575.
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2

Murine Melanoma Cell Culture Protocol

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Murine melanoma cancer cell line B16 that stably expresses OVA was a gift from Dr. Kenneth Rock, Dana-Farber Cancer Institute, Boston. B16-OVA cells were cultured in DMEM supplemented with 10% FBS (GenDEPOT) + 1x 2-mercaptoethanol (BME) + 1:100 penicillin and streptomycin (PS, GenDEPOT). Cells were maintained in a cell incubator at 37°C under 5% CO2.
Hinkle L., Liu Y., Meng C., Chen Z., Mai J., Zhang L., Xu Y., Pan P.Y., Chen S.H, & Shen H. (2021). The Sympathetic Nervous System Modulates Cancer Vaccine Activity Through Monocyte-Derived Cells. Journal of immunology (Baltimore, Md. : 1950), 207(12), 3131-3140.
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3

Gastric Cancer Cell Culture Protocol

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The human gastric carcinoma AGS, MKN45, and MKN74 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). Cells were maintained according to the manufacturer’s instructions. Human gastric carcinoma SNU638 and NCI-N87 cell lines were obtained from the Korean Cell Line Bank (Seoul, Korea). Gastric carcinoma cells and gastric normal HFE-145 cells were grown in RPMI1640 medium (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (Sigma, Darmstadt, Germany) with 100 mg/mL penicillin and streptomycin (GenDEPOT, Barker, TX, USA).
Jeong S., Jo M.J., Yun H.K., Kim D.Y., Kim B.R., Kim J.L., Park S.H., Na Y.J., Jeong Y.A., Kim B.G., Ashktorab H., Smoot D.T., Heo J.Y., Han J., Il Lee S., Do Kim H., Kim D.H., Oh S.C, & Lee D.H. (2019). Cannabidiol promotes apoptosis via regulation of XIAP/Smac in gastric cancer. Cell Death & Disease, 10(11), 846.
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