C3h hej h 2kk

Adult NOD/ShiLtJ (H-2^g7; termed NOD), FVB/NJ (H-2K^q; termed FVB), C3H/HeJ (H-2K^k; termed C3H), and B6.NOD-(D17Mit21-D17Mit10) (H-2^g7; termed B6-g7) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA), bred and housed in a specific pathogen-free facility at the University of Alberta. All care and handling of animals were conducted in accordance with the guidelines of the Canadian Council on Animal Care. All recipient mice used for chimerism induction were females at 8–10 weeks of age. MHC genotypes of the mice used are shown in Table S1 in Supplementary Material.

Six-to-ten-week-old female Balb/c (H2-kd) donor, six-to-eighteen-week-old male and female C57Bl/6 J (H2-kb) recipient, and female C3H/HeJ (H2-kk) third-party mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). All mice were handled and cared for in accordance to the protocol, approved by the Animal Care and Use Committee at National Heart, Lung, and Blood Institute (NHLBI). All research conducted on animals followed biosafety level 2 guidelines. Following BMT, animals were monitored regularly for survival and morbidity (body weight, listlessness, ruffled fur). Mice who were unable to eat or drink and demonstrated persistent deterioration of body weight were euthanized.