Taqman primer gene expression assays

Manufactured by Thermo Fisher Scientific

Sourced in United States

TaqMan primer gene expression assays are a set of pre-designed and validated primers and probes for quantitative real-time PCR (qRT-PCR) analysis of gene expression. They are designed to provide accurate, sensitive, and reproducible results for the detection and quantification of specific gene targets.

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Lab products found in correlation

Itaq universal probes mastermix, by Bio-Rad (2 mentions) Pure link mini rna extraction kit, by Thermo Fisher Scientific (2 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

TaqMan Gene Expression Assays [Primers/probe]; TaqMan Gene Expression Assays primers and probes TaqMan Gene Expression Assays primers TaqMan gene expression assays primers-probe sets TaqMan Gene Expression Assays specific PCR primers sets TaqMan Gene Expression Assays TaqMan gene expression primers TaqMan assay primers TaqMan primer assays TaqMan expression assays

2 protocols using taqman primer gene expression assays

RNA Isolation, cDNA Synthesis, and RT-qPCR Analysis

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mRNA was isolated from up to 100mg of tissue and homogenized in 1mL of lysis buffer reagent provided in the Pure Link Mini RNA extraction kit (Ambion, Austin, TX) as previously described (Boesen et al., 2008 (link)). Following isolation, mRNA concentration was determined using a Nanodrop spectrophotometer (Model 2000, Thermo Scientific, Wilmington, Delaware). cDNA was made using the Biorad cDNA synthesis kit using 1ug of RNA. Finally, real time PCR was carried out using iTaq Universal Probes Mastermix (BioRad) and Taqman primer gene expression assays (Applied Biosystems). Gene expression was quantified using 2^ΔΔCT method.

Heimlich J.B., Speed J.S., Bloom C.J., O'Connor P.M., Pollock J.S, & Pollock D.M. (2014). ET-1 increases reactive oxygen species following hypoxia and high salt diet in the mouse glomerulus. Acta physiologica (Oxford, England), 213(3), 722-730.

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Gene Expression Analysis of Oxidative Stress Markers

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mRNA was isolated using the Pure Link Mini RNA extraction kit (Ambion, Austin, TX) (Boesen, et al 2008 (link)) Real time polymerase chain reaction was carried out using iTaq Universal Probes Mastermix (Biorad, Hercules, CA, USA) and Taqman primer gene expression assays (Applied Biosystems, Foster City, CA, USA; CYBB (NOX2): Mm01287743_m1, NOX4: Mm00479246_m1, EDN1 (ET-1): Mm00438656_m1; GAPDH: Mm999999_g1). Results were quantified using 2^-ΔΔCt and GAPDH served as the internal control (Schmittgen and Livak 2008 (link)).

Zahr R.S., Chappa P., Yin H., Brown L.A., Ataga K.I, & Archer D.R. (2018). Renal Protection by Atorvastatin in a Murine Model of Sickle Cell Nephropathy. British journal of haematology, 181(1), 111-121.

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