The largest database of trusted experimental protocols

2 protocols using nb200 103

1

Immunohistochemical Profiling of Cellular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fixed cells were stained with primary antibody specific for p21 (Novus Biologicals, NBP2-29463), p53 (Novus Biologicals, NB200-103), vimentin (Novus Biologicals, NB300-223), F4/80 (Novus Biologicals, NB600-404), or SMA alpha (Novus Biologicals, NB600-531), followed by a secondary antibody conjugated with chromogenic substrate 3,3’-diaminobenzidine (Santa Cruz Biotechnology). Beta galactosidase activity was assessed in viable cells with X-gal substrate (Cell Biolabs, Inc.) and subsequent staining.
+ Open protocol
+ Expand
2

Celecoxib Cytotoxicity and Apoptosis in HeLa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The sample of this study was prepared from celecoxib (Sigma) with 99.995% purity while cisplatin as positive control was prepared from Cisplatin Kalbe. This subject of this study was HeLa cells, a cell culture collection of Parasitology Department, Faculty of Medicine, Universitas Gadjah Mada. The cells were maintained in Dubelcco Modified Eagle Media (Gibco) containing Fetal Bovine Serum (FBS) 10% (v/v) (Gibco) and penicilinstreptomycin 1% (v/v) (Gibco). Cytotoxic assay was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) (Sigma) method while the apoptosis assay was examined by ethidium bromide and acridine orange (Sigma) staining. Propidium iodide reagent contained 0.1% triton X (E.Merck, Germany), 0.2% RNAse and 5% Propidium iodide (Sigma) in Phosphate Buffer Saline (PBS). Immunocytochemistry used p53 primary antibody purchased form Novus Bio NB 200-103, while the universal secondary antibody was derived from Starr Trek Universal HRP Detection System No.901-STUHRP700-090314. All culture plates used in this study were Iwaki® and all tips and micro tubes were supplied by Axigen®.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!