All chromatographic evaluations were performed using ACQUITY UPLC Classic, H‐Class, I‐Class, or Premier Systems equipped with ACQUITY photodiode array detectors (Waters Corporation, Milford, MA, USA). ACQUITY UPLC BEH Amide, BEH HILIC, and Atlantis Premier BEH Z‐HILIC columns (1.7 μm, 2.1 × 50 mm) were obtained from Waters (Milford, MA, USA).
Beh hilic
Manufactured by Waters Corporation
Sourced in United States
The BEH HILIC is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of polar and hydrophilic compounds. It uses a proprietary hybrid particle technology to provide enhanced resolution and sensitivity.
Automatically generated - may contain errors
Lab products found in correlation
Acquity classic uplc, by Waters Corporation (1 mentions)
Acquity photodiode array detector, by Waters Corporation (1 mentions)
Premier, by Waters Corporation (1 mentions)
I class, by Waters Corporation (1 mentions)
H class, by Waters Corporation (1 mentions)
Acquity uplc beh amide, by Waters Corporation (1 mentions)
Propranolol hydrochloride, by Merck Group (1 mentions)
Imipramine hydrochloride, by Merck Group (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Ammonium hydroxide solution, by Merck Group (1 mentions)
Apocynin, by Merck Group (1 mentions)
Eugenol, by Merck Group (1 mentions)
4 hydroxybenzyl alcohol, by Merck Group (1 mentions)
Torus diol, by Waters Corporation (1 mentions)
O cresol, by Merck Group (1 mentions)
2 4 6 trimethylphenol, by Merck Group (1 mentions)
Methyl tert butyl ether (mtbe), by Thermo Fisher Scientific (1 mentions)
Methanol meoh, by Thermo Fisher Scientific (1 mentions)
M cresol, by Merck Group (1 mentions)
Phenol, by Merck Group (1 mentions)
3 protocols using beh hilic
1
UPLC Chromatographic Evaluation Methods
2
Analytical Method Development for Phytochemicals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All standards had a purity exceeding 95%. The following standards were obtained from Sigma Aldrich (Germany): eugenol, 2,4,6-trimethylphenol, 1-indanol, apocynin, o-cresol, m-cresol, phenol, 4hydroxybenzyl alcohol, naringenin, acrylic acid, imipramine hydrochloride and propranolol hydrochloride. Arbutin, trans-cinnamic acid and ferulic acid were obtained from Fluka (UK), Sigma Acros Organic (USA) and Sarsyntex (France), respectively.
Methanol (MeOH) (≥ 99.8 %), ethanol (> 98 %) and formic acid (> 98 %) were purchased from Fisher Scientific (UK), acetonitrile (ACN) (≥ 99.9 %) was purchased from Honeywell (Germany), methyl tertbutyl ether (MtBE) (≥ 99.9 %) was purchased from Acros Organics (USA).
Ultrapure water was delivered by PURELAB Classic system from Elga (UK) (18.2 MΩ-cm).
Ammonium hydroxide solution was ACS reagent (28.0-30.0 % NH 3 basis) purchased from Sigma-Aldrich (Germany). Pressurized carbon dioxyde (CO 2 ) (N45, ≥ 99,995 %) was purchased from Air Liquide (France).
Three columns were assessed, Torus DEA and Torus Diol with dimensions 100 x 3.0 mm, 1.7 µm and BEH HILIC with dimensions 100 x 2.1 mm; 1.7 µm, all from Waters (Milford, USA).
Methanol (MeOH) (≥ 99.8 %), ethanol (> 98 %) and formic acid (> 98 %) were purchased from Fisher Scientific (UK), acetonitrile (ACN) (≥ 99.9 %) was purchased from Honeywell (Germany), methyl tertbutyl ether (MtBE) (≥ 99.9 %) was purchased from Acros Organics (USA).
Ultrapure water was delivered by PURELAB Classic system from Elga (UK) (18.2 MΩ-cm).
Ammonium hydroxide solution was ACS reagent (28.0-30.0 % NH 3 basis) purchased from Sigma-Aldrich (Germany). Pressurized carbon dioxyde (CO 2 ) (N45, ≥ 99,995 %) was purchased from Air Liquide (France).
Three columns were assessed, Torus DEA and Torus Diol with dimensions 100 x 3.0 mm, 1.7 µm and BEH HILIC with dimensions 100 x 2.1 mm; 1.7 µm, all from Waters (Milford, USA).
3
Quantification of Pharmaceutical Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
N-acetyl-L-leucine, rosuvastatin, estrone-3-sulfate and chlorothiazide were separated and quantified using a Thermo Vanquish UPLC + Thermo Quantis triple quadrupole MS on a Waters Acquity HSS T3 (2.1 × 50 mm, 1.7 μm) column with guard filter. A sample of 4 µL was injected and compounds were eluted at 35°C with a flow of 0.65 mL/min using a gradient of solvent A = 0.1% formic acid and solvent B = acetonitrile as follows (Time, A%): 0. 0,95;0.5,95;2.5,40;3.5,5;4.5,95. Metformin was separated and quantified using a Waters Acquity UPLC + Waters XEVO triple quadrupole MS on a Waters Acquity BEH HILIC (2.1 × 50 mm, 1.7 μm) column with guard filter. A sample of 4 µL was injected and compounds were eluted at 35°C with a flow of 0.5 mL/min using a gradient of solvent A = 10 mM ammonium formate and solvent B = acetonitrile as follows (Time, A%): 0.0, 5; 0.5, 5; 2.5, 50; 3.5, 50; 4.5, 5.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!