T47D cells were labeled overnight with CellLights BacMam 2.0 MitoGFP (Thermo Fisher, Waltham, MA, USA) according to manufacturer’s protocol. Cells were treated for 24 h with vehicle (EtOH), E2 (10−8 M) or P4 (10−8), or E2 + P4 for 24 h then were fixed in 4% paraformaldehyde, counterstained with DAPI, and mounted on coverslips. Images were collected using confocal laser scanning microscopy (Zeiss LSM 780) with 40× objective.
Celllights bacmam 2.0 mitogfp
Manufactured by Thermo Fisher Scientific
Sourced in United States
CellLights BacMam 2.0 MitoGFP is a fluorescent labeling reagent that specifically labels mitochondria in live cells. It contains a baculovirus vector that expresses a mitochondrial-targeted green fluorescent protein (GFP) under the control of a constitutive promoter.
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Lab products found in correlation
3 protocols using celllights bacmam 2.0 mitogfp
1
Mitochondrial Dynamics in Breast Cancer Cells
2
Mitochondrial labeling in T47D cells
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T47D cells were labeled overnight with CellLights BacMam 2.0 MitoGFP (Thermo Fisher, Waltham, MA) according the manufacturer's protocol. Following treatment, cells were xed in 4% paraformaldehyde, counterstained with DAPI, and mounted on coverslips. Images were collected using confocal laser scanning microscopy (Zeiss LSM 780) with 40X objective.
3
Visualizing Mitochondria and Lipid Droplets in T47D Cells
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T47D cells were labeled overnight with CellLights BacMam 2.0 MitoGFP (Thermo Fisher, Waltham, MA) according to manufacturer's protocol. Cells were treated for 24 h with vehicle (EtOH), E2 (10-8 M) or P4 , or E2 + P4 for 24 h then were xed in 4% paraformaldehyde, counterstained with DAPI, and mounted on coverslips. Images were collected using confocal laser scanning microscopy (Zeiss LSM 780) with 40X objective. 2.7. Lipid droplet staining and quantitation T47D or UCD4 cells were plated on glass coverslips at 1.5x104 cells/well in phenol-free medium in the presence of vehicle, E2 (10-8 M), R5020 (10-8 M), or E2 plus R5020 (10-8 M each) for 5 days.
Coverslips were xed (10% buffered formalin), stained with Oil Red O in propylene glycol (PEG), counterstained with hematoxylin, mounted on slides, and imaged at 40X magni cation (Olympus BX40). Neutral lipid stain was quanti ed on ImageJ and normalized to cell area (4 elds per condition).
Coverslips were xed (10% buffered formalin), stained with Oil Red O in propylene glycol (PEG), counterstained with hematoxylin, mounted on slides, and imaged at 40X magni cation (Olympus BX40). Neutral lipid stain was quanti ed on ImageJ and normalized to cell area (4 elds per condition).
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