Rag0072

Manufactured by MultiSciences Biotech

Sourced in China

RAG0072 is a compact, automated laboratory instrument designed for high-throughput sample processing. It features a multi-channel pipetting system, integrated temperature control, and advanced data management software. The core function of RAG0072 is to perform precise liquid handling and sample preparation tasks, enabling efficient and standardized workflows in various biotech applications.

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Lab products found in correlation

2 protocols using rag0072

Antibody Profiling in Western Blots

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Antibodies used in Western blots were as the following: anti-β-actin (A5316, Sigma), anti-tubulin (T6734, Sigma), anti-MIG6 (WH0054206M1, Sigma), anti-pTyr239/240 SHC1 (2434, Cell Signaling), anti-SHC1 (610878, BD Bioscience), anti-phospho-p44/42 MAPK (9101, Cell Signaling), anti-p44/42 MAPK (9102, Cell Signaling), anti-GST (2625, Cell Signaling), and anti-HA tag (A01244, GenScript). Immunoreactivity was detected using horseradish-peroxidase (HRP)-conjugated secondary antibody (RAG0072 for anti-rabbit, GAM0072 for anti-mouse, 1:5,000 dilution, Multi Sciences, Hangzhou, China).

Liu L., Xing L., Chen R., Zhang J., Huang Y., Huang L., Xie B., Ren X., Wang S., Kuang H., Lin X., Kumar A., Kim J.K., Lee C, & Li X. (2021). Mitogen-Inducible Gene 6 Inhibits Angiogenesis by Binding to SHC1 and Suppressing Its Phosphorylation. Frontiers in Cell and Developmental Biology, 9, 634242.

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Comprehensive Protein Analysis Workflow

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Western blots were performed by separating proteins on SDS-PAGE gels followed by protein transfer to PVDF membranes (Bio-Rad). The antibodies used were: anti-PDGFD (sc-137030, Santa Cruz), anti-SOX2 (AF-2018, R&D), anti-BRACHYURY (sc-166962, Santa Cruz), anti-ERKs (4696, Cell Signaling Technology), anti-Phospho ERKs (4370, Cell Signaling Technology), anti-PDGFR-β (3169, Cell Signaling Technology), anti-Phospho PDGFR-β (Tyr1021) (2227, Cell Signaling Technology), anti-PECAM1 (222783, Abcom), anti-Phospho STAT3 (9145, Cell Signaling Technology), anti-STAT3 (9139, Cell Signaling Technology), anti-KDR (9698, Cell Signaling Technology), anti-HSP90 (7411, Cell Signaling Technology), anti-TUBULIN (RM2007, Ray Antibody), anti-β-ACTIN (RM2001, Ray Antibody), anti-GAPDH (70-Mab5465-040, MultiSciences), anti-HISTONE 3 (GB13102-1, Servicebio), Goat anti-mouse IgG (GAM0072, MultiSciences), Goat anti-rabbit IgG (GAR0072, MultiSciences), Rabbit anti-goat IgG (RAG0072, MultiSciences), anti-PDGFB (PA1-27394, Invitrogen), anti-uPA (17968-1-AP, Proteintech) and anti-PLASMIN (66399-1-Ig, Proteintech). The bands were visualized using a GBOX-CHEMI-XX8 device (SYNGENE).

Lu W., Xu P., Deng B., Zhang J., Zhan Y., Lin X., Xu X., Xia Z., Yang X., Zeng X., Huang L., Xie B., Wang C., Wang S., Kuang H., Han X., Mora A., Cao Y., Jiang Q, & Li X. (2022). PDGFD switches on stem cell endothelial commitment. Angiogenesis, 25(4), 517-533.

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