Para nitrophenyl butyrate

Impranil DLN was supplied by Bayer Corporation (Germany). Poly[4-(2,2-dicyanovinyl)-N-bis(hydroxyethyl)aniline-alt-(4,4’-methylenebis(phenylisocyanate)))]urethane and poly[4-(2,2-dicyanovinyl)-N-bis(hydroxyethyl)aniline-alt-(isophroronediisocyanate)]urethane were purchased from Sigma Aldrich (France).
Tween 20, Pefabloc, para-nitrophenyl acetate (pNPA), para-nitrophenyl butyrate (pNPB), para-nitrophenyl palmitate (pNPP), para-nitrophenol (pNP), tetrahydrofuran (THF), 2-sec-butylphenol, (2-Butan-2-ylphenyl), N-methylcarbamate (fenobucarb), Ethyl 2-(4-phenoxyphenoxy)ethylcarbamate (fenoxycarb) and S-Benzyl dipropylthiocarbamate (prosulfocarb) were purchased from Sigma Aldrich (France).
AZCL-xylan (XYL), AZCL-casein (CAS), AZCL-Barley β-glucan (BGLU), AZO-CM Cellulose (CMC), AZO-Carob Galactomannan (GM) were purchased from Megazyme (Ireland).
Lysonase™ Bioprocessing Reagent was purchased from Novagen (France).

Buffer components and model substrates such as para-nitrophenyl-butyrate (pNPB), para-nitrophenyl-N-benzylcarbamate (pNPC), butyl-N(para-nitrophenyl)carbamate (pNPBC) and para-nitrobutyranilide (pNPA) were purchased from Sigma-Aldrich (Vienna, Austria). All other chemicals and reagents used in this work were of analytical grade and used without further purification if not otherwise specified. Humicola insolens cutinase (HiC) was purchased from STREM chemicals (BISCHHEIM, France, product code 06-3135, Novozym 51032, CAS Number: 9001-62-1). Commercial polyurethane pellets (PU1080) were kindly provided by Bayer (Leverkusen, Germany).