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Gg8 1f3

Manufactured by Merck Group
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The GG8-1F3.3 is a laboratory equipment product from Merck Group. It is a versatile instrument designed for various scientific applications. The core function of the GG8-1F3.3 is to perform precise measurements and analysis, providing reliable data for researchers and scientists.

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Lab products found in correlation

Immobilon p transfer membrane, by Merck Group (2 mentions) Restore western blot stripping buffer, by Thermo Fisher Scientific (2 mentions) Ab108296, by Cell Signaling Technology (2 mentions) 1mm criterion precast gels, by Bio-Rad (2 mentions) Hrp conjugated bovine anti goat, by Jackson ImmunoResearch (2 mentions) Non fat dry milk, by Santa Cruz Biotechnology (2 mentions) Nupage bis tris gel, by Thermo Fisher Scientific (2 mentions) Hrp conjugated donkey anti rabbit, by GE Healthcare (2 mentions)

2 protocols using gg8 1f3

1

Western Blot Protein Detection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates were run on Tris-HCl, 1mm Criterion Precast gels (Bio-Rad) or NuPAGE Bis-Tris gels (Novex) gels at a constant voltage. Proteins were transferred onto Immobilon-P transfer membranes (Millipore) at a constant amperage. Before staining with primary antibodies, blots were blocked in 5% non-fat dry milk (Santa Cruz) or 5% BSA in TBS-T 0.1% for 30 minutes.
For protein detection primary antibodies detecting CK1α (C-19, Santa Cruz or Abcam ab108296), β-catenin (Cell Signaling #9587 and #8480), HA (HRP-conjugate, Miltenyi, GG8-1F3.3), FLAG (M2, HRP-conjugate Sigma Aldrich), ubiquitin conjugates (FK2, HRP-conjugate Enzo Life Sciences), Actin (HRP-conjugate, Abcam), β-tubulin (Cell Signaling #2146) and GAPDH (Santa Cruz sc-47724) were used. Secondary antibodies were HRP conjugated Bovine anti-Goat (Jackson ImmunoResearch) and HRP conjugated donkey anti-rabbit (GE Healthcare). SuperSignal (Thermo Scientific) chemi-luminescent substrate was used for detection. For re-probing, blots were stripped in Restore Western Blot Stripping Buffer (Thermo Scientific), activated in methanol, and re-blocked.
Krönke J., Fink E.C., Hollenbach P.W., MacBeth K.J., Hurst S.N., Udeshi N.D., Chamberlain P.P., Mani D.R., Man H.W., Gandhi A.K., Svinkina T., Schneider R.K., McConkey M., Järås M., Griffiths E., Wetzler M., Bullinger L., Cathers B.E., Carr S.A., Chopra R, & Ebert B.L. (2015). Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDS. Nature, 523(7559), 183-188.
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2

Western Blot Protein Detection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates were run on Tris-HCl, 1mm Criterion Precast gels (Bio-Rad) or NuPAGE Bis-Tris gels (Novex) gels at a constant voltage. Proteins were transferred onto Immobilon-P transfer membranes (Millipore) at a constant amperage. Before staining with primary antibodies, blots were blocked in 5% non-fat dry milk (Santa Cruz) or 5% BSA in TBS-T 0.1% for 30 minutes.
For protein detection primary antibodies detecting CK1α (C-19, Santa Cruz or Abcam ab108296), β-catenin (Cell Signaling #9587 and #8480), HA (HRP-conjugate, Miltenyi, GG8-1F3.3), FLAG (M2, HRP-conjugate Sigma Aldrich), ubiquitin conjugates (FK2, HRP-conjugate Enzo Life Sciences), Actin (HRP-conjugate, Abcam), β-tubulin (Cell Signaling #2146) and GAPDH (Santa Cruz sc-47724) were used. Secondary antibodies were HRP conjugated Bovine anti-Goat (Jackson ImmunoResearch) and HRP conjugated donkey anti-rabbit (GE Healthcare). SuperSignal (Thermo Scientific) chemi-luminescent substrate was used for detection. For re-probing, blots were stripped in Restore Western Blot Stripping Buffer (Thermo Scientific), activated in methanol, and re-blocked.
Krönke J., Fink E.C., Hollenbach P.W., MacBeth K.J., Hurst S.N., Udeshi N.D., Chamberlain P.P., Mani D.R., Man H.W., Gandhi A.K., Svinkina T., Schneider R.K., McConkey M., Järås M., Griffiths E., Wetzler M., Bullinger L., Cathers B.E., Carr S.A., Chopra R, & Ebert B.L. (2015). Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDS. Nature, 523(7559), 183-188.
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