Multi species glp 1 total elisa kit ezglp1t 36k

The intestinal tissues were immersed in an acid alcohol solution (74 % absolute ethanol, 25 % water and 1 % HCl 12 N) using 5 ml/g tissue weight (33) . Next, intestinal tissues were sectioned into small segments and subsequently homogenised at 24 000 rpm (Ultra Turrax homogenizer T18, IKA) for 2 min. The homogenised samples were extracted at 4°C for 24 h. After extraction, 150 μl of each homogenised sample was stored at -30°C for measuring the protein concentration. Subsequently, the remaining homogenate was centrifuged at 2000 g for 20 min. The supernatant was collected and stored at -80°C for further analysis. The protein concentration and GLP-1 content were measured using Lowry's protein assay and Multi Species GLP-1 Total ELISA kit (EZGLP1T-36 K; Merck Millipore), respectively. The homogenate samples were diluted forty times for measurement of protein concentration, whereas the supernatant was diluted 500 times for the measurement of GLP-1 content.