Phospho ser93 beclin1

Manufactured by Cell Signaling Technology

Phospho-Ser93-beclin1 is an antibody that specifically recognizes the Serine 93 phosphorylated form of the Beclin 1 protein. Beclin 1 is a key regulator of autophagy, a cellular process involved in the degradation and recycling of damaged organelles and proteins. The phosphorylation of Beclin 1 at Serine 93 is believed to play a role in the regulation of autophagy, though the exact mechanisms are still under investigation.

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Lab products found in correlation

Ampkα1, by Cell Signaling Technology (1 mentions) Hoechst dye, by Thermo Fisher Scientific (1 mentions) Parkin, by Santa Cruz Biotechnology (1 mentions) 5 aminoimidazole 4 carboxamide 1 β d ribofuranoside, by Enzo Life Sciences (1 mentions) Emulsion oil solution containing 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (1 mentions) A769662, by MedChemExpress (1 mentions) Mk 8722, by MedChemExpress (1 mentions) Phospho acetyl coa carboxylase ser79, by Cell Signaling Technology (1 mentions) Phospho ampk thr172, by Cell Signaling Technology (1 mentions) Rpmi 1640, by Merck Group (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Metformin, by Merck Group (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Aldh1a1, by Cell Signaling Technology (1 mentions) Rapamycin, by Selleck Chemicals (1 mentions) Beclin 1, by Cell Signaling Technology (1 mentions) 3 methyladenine, by Selleck Chemicals (1 mentions) Anti lc3 2, by Cell Signaling Technology (1 mentions) Spautin 1, by Selleck Chemicals (1 mentions) Sqstm1 p62, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-phospho Beclin1-Ser93/Ser96 (2 citations)

2 protocols using phospho ser93 beclin1

AMPK Activation and Autophagy Regulation

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Metformin, RPMI 1640, and LPS were from Sigma-Aldrich (St. Louis, MO). 5-Aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) was purchased from Enzo (Farmingdale, NY). AMPK activators MK8722 and A769662 were from MedChemExpress (Monmouth Junction, NJ). Antibodies: phospho-Thr172-AMPK, AMPKα1, phospho-Ser93-beclin1, phospho-Ser79-Acetyl CoA Carboxylase, and LC3B were obtained from Cell Signaling Technology (Beverly, MA). Parkin and β-actin were from Santa Cruz Biotechnology (Dallas, TX), whereas anti-T1α IgG from R&D Systems (Minneapolis, MN). Horse Radish Peroxidase-conjugated antibodies were obtained from Bio-Rad (Hercules, CA). Emulsion oil solution containing 4′,6-diamidino-2-phenylindole (DAPI) from Vector Laboratories (Burlingame, CA). Hoechst dye from Life Technologies (Grand Island, NY) was applied to visualized nuclei in live macrophages.

Bone N.B., Becker EJ J.r., Husain M., Jiang S., Zmijewska A.A., Park D.W., Chacko B., Darley-Usmar V., Grégoire M., Tadie J.M., Thannickal V.J, & Zmijewski J.W. (2021). AMPK activates Parkin independent autophagy and improves post sepsis immune defense against secondary bacterial lung infections. Scientific Reports, 11, 12387.

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Characterization of Autophagy Regulation

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Osimertinib (TAGRISSO) was obtained from Astra Zeneca. Spautin-1 (S7888), 3-Methyladenine (S2767) and rapamycin (S1039) were purchased from Selleck. Cycloheximide (C6628) was purchased from Sigma-Aldrich, and Cycloheximide (HY-12320) from MedChem Express. Anti-LC3II (#12741S), SQSTM1/p62 (#8025S), Atg5 (#9980S), Beclin 1 (#3495S), phospho-(Ser93)-beclin1 (#14717S), Sox2 (#3579S), ALDH1A1 (#36671S), GAPDH (#2118S) antibodies were from Cell Signaling Technology.

Li L., Wang Y., Jiao L., Lin C., Lu C., Zhang K., Hu C., Ye J., Zhang D., Wu H., Feng M, & He Y. (2019). Protective autophagy decreases osimertinib cytotoxicity through regulation of stem cell-like properties in lung cancer. Cancer letters, 452.

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