The paraffin sections were deparaffinized, dehydrated, and heated in citrate buffer for antigen retrieval. After blocking with 10% goat serum (ZSGB-BIO, Beijing, China) for 1 h, the sections were incubated overnight with rabbit antinephrin (1 : 50, sc-377246, Santa Cruz Biotech, CA, USA) antibody and rabbit anti-caspase-1 antibody (1 : 200, ab1872) at 4°C, followed by FITC-conjugated goat anti-rabbit secondary antibody (1 : 100; clone ZF-0311, ZSGB-BIO, Beijing) for 1 h at 37°C. The sections were counterstained with 4′, 6′-diamidino-2-phenylindole (DAPI) (Genview, Florida, USA) and imaged using a laser confocal microscope (TCS SP8 STED, Leica, Wetzlar, Germany). Alternatively, the podocytes were permeabilized with Triton X-100, washed thrice with PBS, and stained with 100 μg/mL phalloidin-conjugate working solution (AAT Bioquest, Sunnyvale, CA, USA) for 1 h at room temperature. The stained cells were washed thrice with PBS and observed under a laser scanning confocal microscope.
Fitc conjugated goat anti rabbit secondary antibody
Manufactured by ZSGB-BIO
Sourced in United States
FITC-conjugated goat anti-rabbit secondary antibody is a laboratory reagent used to detect and visualize rabbit primary antibodies in various immunoassay techniques. It consists of goat-derived antibodies that have been conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate), allowing for the specific detection of rabbit antibodies through fluorescence microscopy or flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Tcs sp8 sted, by Leica (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Genview (1 mentions)
Phalloidin conjugate working solution, by AAT Bioquest (1 mentions)
Sc 377246, by Santa Cruz Biotechnology (1 mentions)
Facscanto 2 flow cytometer, by BD (1 mentions)
Ebioscience foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions)
Modfit version 3.1, by Verity Software House (1 mentions)
2 protocols using fitc conjugated goat anti rabbit secondary antibody
1
Immunocytochemistry of Podocyte Proteins
2
Apoptosis and Cell Cycle Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were treated with BIBR1532 or exposed to radiation, or both. Annexin V-FITC and propidium iodide stains were used for apoptosis analysis. Cells were stained in the dark for 15 minutes at room temperature. For cell cycle analysis, cells were collected and fixed in 70% precooled ethanol overnight at 4 C. Cells were stained with a propidium iodideeRNase staining buffer at room temperature for 15 minutes and then analyzed using flow cytometry. Samples were analyzed using ModFit version 3.1 software (Verity Software House). For quantifying pHH3-positive cells, cells were fixed and permeated using eBioscience Foxp3/ Transcription Factor Staining Buffer Set (Thermo Fisher) for 1 hour in the dark at 4 C, incubated with pHH3 antibody (1:200), probed with the FITC-conjugated goat antirabbit secondary antibody (1:400; ZSGB-BIO, Beijing, China), and stained with a propidium iodideeRNase staining buffer. Each sample was then subjected to analyses with flow cytometry (BD FACSCanto II Flow Cytometer; BD Biosciences, Bedford, MA).
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