Turkey red blood cells trbcs

Dulbecco’s Modified Eagles Medium (DMEM), fetal bovine serum (FBS), L-glutamine, penicillin/streptomycin (P/S), Opti-MEM I (OMEM), anti-goat IgG HRP-linked secondary antibody, Simple Blue Stain, Novex 4–12% Tris-Glycine SDS-PAGE gels, dithiothreitol (DTT) Immulon-2HB 96-well plates, Nickel coated 96-well plates and Lipofectamine 2000 transfection reagent were all purchased from Thermo Fisher Scientific. Zanamivir and 2’-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid (MUNANA) were acquired from Moravek Inc and Cayman Chemicals, respectively. β-propiolactone, formaldehyde, o-Phenylenediamine dihydrochloride (OPD), and anti-mouse IgG HRP linked secondary were purchased from Sigma. Anti-rabbit IgG HRP-linked secondary antibody and 0.45-μm polyvinylidene difluoride (PVDF) membrane were obtained from GE healthcare. Specific-Pathogen-Free (SPF) eggs and turkey red blood cells (TRBCs) were purchased from Charles River Labs and the Poultry Diagnostic and Research Center (Athens, GA), respectively. The H1N1 A/Brisbane/02/2018 field isolate (WT) and CVV (IVR-190) were kindly provided by the WHO. Rabbit Antisera against NA was generated by Agrisera (Sweden) using NA-WSN residues 35–453 isolated from E. coli inclusion bodies [29 (link)]. Polyclonal goat antiserum against the H1N1 influenza virus A/Fort Monmouth/1/1947 (NR-3117) was obtained from BEI Resources, NIAID, NIH.

Madin-Darby canine kidney 2 cells (MDCK.2; CRL-2936) and HEK 293 T/17 cells (CRL-11268) were purchased from LGC Standards. Dulbecco’s Modified Eagles Medium (DMEM), L-glutamine, penicillin/streptomycin (P/S), Opti-MEM I (OMEM), Simple Blue Stain, Novex 4–12% Tris-Glycine SDS-PAGE gels, Maxisorp 96-well plates and Lipofectamine 2000 transfection reagent were obtained from Thermo Fisher Scientific. Fetal bovine serum (FBS), 2′-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid (MUNANA) and Zanamivir were acquired from Atlanta Biologicals, Cayman Chemical and Moravek Inc, respectively. Bovine fetuin, o-phenylenediamine dihydrochloride (OPD), and HRP-linked peanut agglutinin were purchased from Sigma. Specific-pathogen-free (SPF) eggs and turkey red blood cells (TRBCs) were purchased from Charles River Labs and the Poultry Diagnostic and Research Center (Athens, GA), respectively.