FLAG-M2 (F3165) and TUBB (T8453) were purchased from Sigma Aldrich (St. Louis, MO). TCF7L2 (2569), acH3K9 (9649), CBP (7389), FOXP1 (4402), β-catenin (8480), Lys49 acetylated-β-catenin (9534) and rabbit IGG (3900) were all purchased from Cell Signaling (Danvers, MA). LEF1 (A303-486A) is from Bethyl Laboratories (Montgomery, TN). Mouse β-catenin (610153) antibody was from BD transduction. CT99021 was purchased from Axon Med Chem (Groningen, Netherlands). Trichostatin A, Doxorubucin HCL, Etoposide, XAV938, Anacardic Acid, and CPTH2 were from Sigma Aldrich (St. Louis, MO). DKK1, Wnt3a, TNFα, and TGFβ1 were purchased from Peprotech (Rocky Hill, NJ). C-59 was purchased from Cellagen Technology (San Diego, CA). 4-Hydroxycyclophosphamide was a gift of Howard McLeod. Wnt3a conditioned media was made according to the ATCC protocol.
Rabbit igg 3900
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit IgG (3900) is a purified immunoglobulin G (IgG) fraction from rabbit serum. It is commonly used as a secondary antibody in various immunological techniques, such as Western blotting, ELISA, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Anacardic acid, by Merck Group (1 mentions)
Trichostatin a, by Merck Group (1 mentions)
Cpth2, by Merck Group (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Wnt3a, by Thermo Fisher Scientific (1 mentions)
Tgf β1, by Thermo Fisher Scientific (1 mentions)
Etoposide, by Merck Group (1 mentions)
Ab8895, by Abcam (1 mentions)
Ab1220, by Abcam (1 mentions)
Ab91252, by Abcam (1 mentions)
Ab4729, by Abcam (1 mentions)
Epiquik tissue chip kit, by Epigentek (1 mentions)
2 protocols using rabbit igg 3900
1
Reagents and Treatments for Wnt Signaling
2
ChIP-seq Protocol for Epigenetic Modifications
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were treated using the EpiQuik Tissue ChIP Kit (48 reactions) (P-2003-2, Epigentek, USA). Con uent cells at 70-80% con uency were xed with 1% formaldehyde at room temperature for 10 min to generate the intracellular DNA-protein crosslink, which was then randomly broken by ultra-sonication into fragments. Cell fragments were centrifuged at 13,000 g at 4℃, followed by the division of supernatant into three tubes, respectively, which was separately added with antibody RNA polymerase II (positive control), mouse antibody immunoglobulin G (IgG) (1 mg/mL) or rabbit IgG (3900, Cell Signaling Technology, USA) (negative control) or antibodies against KDM3A (ab91252, Abcam, Cambridge, UK), H3K27me3 (ab1220, Abcam, UK), CTGF (SimpleChIP Human CTGF Promoter Primers #14927, USA), H3K27ac (ab4729, Abcam) and H3K4me1 (ab8895, Abcam). After immunoprecipitation, de-crosslink was performed and proteins were treated by proteinase K. DNA was eluted and puri ed using Active Motif's ChIP DNA puri cation kit (58002, Millipore). Each experiment was repeated 3 times.
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