Sasi hs01 00176980

Manufactured by Merck Group

SASI_Hs01_00176980 is a laboratory equipment product from Merck Group. It is a specialized device designed for performing various scientific experiments and analyses within a controlled laboratory environment. The core function of this product is to facilitate the collection, processing, and analysis of research data. Additional details about its intended use or specific capabilities are not available.

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Lab products found in correlation

On targetplus sirna, by Horizon Discovery (1 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)

2 protocols using sasi hs01 00176980

siRNA Transfection for SUN1, SUN2, and MAD2

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Cells were transfected using Lipofectamine RNAiMAX reagent (Invitrogen) according to the manufacturer’s instructions (protocol publication no. MAN0007825 Rev.1.0) with the following siRNAs:
SUN2 rat [Unc84bRSS342353(3_RNAI), Invitrogen]:

Forward (Fw): 5′-GAC UCG GAA GAC CUA UUC AAG AAG A-3′

Reverse (Rv): 5′-U CUU CUU GAA UAG GUC UUC CGA GUC-3′

SUN2 human: SASI_Hs01_00176980 (Sigma-Aldrich)
SUN2#2 human: Silencer Select Predesigned s24467 (Ambion, Thermo Fisher Scientific):

Fw: 5′-CCUUAGAGCAUGUGCCCAAtt-3′

Rv: 5′-UUGGGCACAUGCUCUAAGGta-3′

MAD2 human: Silencer Select Predesigned s8392 (Ambion, Thermo Fisher Scientific):

Fw: 5′-CGCCUUCGUUCAUUUACUAtt-3′

Rv: 5′-UAGUAAAUGAACGAAGGCGga-3′

MAD2#2 human: Silencer Select Predesigned s8393 (Ambion, Thermo Fisher Scientific):

Fw: 5′-CCUUUACUCGAGUGCAGAAtt-3′

Rv: 5′-UUCUGCACUCGAGUAAAGGtt-3′

SUN1#1 human: ON-TARGETplus siRNA (Dharmacon)

Fw: 5′-AUGUUGAAUUGGACGGCCA-3′

Rv: 5′-UGGCCGUCCAAUUCAACAU-3′

SUN1#2 human: ON-TARGETplus siRNA (Dharmacon)

Fw: 5′-GUAUAUACCAAGACGCCAU-3′

Rv: 5′-AUGGCGUCUUGGUAUAUAC-3′

Control siRNA: Silencer Negative Control No. 1 siRNA (AM4635 Ambion, Thermo Fisher Scientific).

Belaadi N., Pernet L., Aureille J., Chadeuf G., Rio M., Vaillant N., Vitiello E., Lafanechère L., Loirand G, & Guilluy C. (2022). SUN2 regulates mitotic duration in response to extracellular matrix rigidity. Proceedings of the National Academy of Sciences of the United States of America, 119(45), e2116167119.

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Targeted Knockdown of Nuclear Proteins

Cited 1 time

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Lipofectamine RNAiMAX (Invitrogen) was used to transfect 10 nM dsRNA following manufacturers protocol. 48 h post transfection, cells were used for assays. To down regulate PREP1, a cocktail of two siRNAs were used (siRNA 607 = GAUUUCUGCAGUCGAUACA; siRNA 900 = CUCCCAGCUUCAGUUACAG)^{11 (link)}. To target LMNB1 10 nM siRNA duplexes of abx903005 (Abbexa Ltd LMNB1: GCAGACUUACCAUGCCAAA) was used. To target SUN1, a Mission siRNA (Sigma-Aldrich SASI_Hs01_00032809) with the sequence CAGCUAAAGUCAGAGCUGU was used. To target SUN2 a Mission siRNA (Sigma-Aldrich SASI_Hs01_00176980) with the sequence CUAUUCAGACGUUUUCACUU was used. An siRNA targeting firefly luciferase (CAUCACGUACGCGGAAUAC) was used as control in all experiments.

Purushothaman D., Bianchi L.F., Penkov D., Poli A., Li Q., Vermezovic J., Pramotton F.M., Choudhary R., Pennacchio F.A., Sommariva E., Foiani M., Gauthier N., Maiuri P, & Blasi F. (2022). The transcription factor PREP1(PKNOX1) regulates nuclear stiffness, the expression of LINC complex proteins and mechanotransduction. Communications Biology, 5, 456.

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