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Mats u52ra26

Manufactured by Tokai Hit
Sourced in Japan

The MATS-U52RA26 is a laboratory equipment designed for precise temperature control and measurement. It features a temperature range of -40°C to 200°C and can maintain temperature stability within ±0.1°C. The equipment is intended for use in research and scientific applications requiring accurate temperature regulation.

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2 protocols using mats u52ra26

1

Rabai–Kaminaga–Hanazaki Reaction Model

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The following is a simple reaction model of the BSF reaction that is called the Rabai–Kaminaga–Hanazaki model34 35 (link):





For Figs 3, 4f,h and 5, aqueous phase 1 consisted of 150-mM KBrO3 (Wako Pure Chemical) and 0.2-mM Fl–Na, whereas aqueous phase 2 was 30-mM K4Fe(CN)6 (Wako Pure Chemical), 15-mM H2SO4 (Nacalai Tesque) and Na2SO3 (Wako Pure Chemical). Thus, the final concentrations in the transporters were , , and 0.1-mM Fl–Na. was varied depending on the experiment being performed. The initial chemical concentrations in the reactor were the same as those in the transporters. All the experiments were carried out at ∼35 °C using a thermo-plate (MATS-U52RA26, Tokai Hit) on the microscope.
We used the Rabai–Kaminaga–Hanazaki model for the numerical analyses in Fig. 3e,g. The numerical analyses were performed using Mathematica (Wolfram Research). The details are given in Supplementary Note 2.
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2

Microfluidic Droplet Bioreactor for E. coli

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The oil phase was a silicone oil (KF-96L-1CS; Shin-Etsu Chemical, Tokyo, Japan), with a 2% (w/w) surfactant (KF-6028; Shin-Etsu Chemical). The aqueous phase of the reactor droplet introduced into the square chamber was the E. coli culture solution (the 100-fold diluted solution of an overnight culture of E. coli). The aqueous phase of the carrier droplets was the LB medium containing antibiotics. The flow rates of the oil and the aqueous phases were both set at 8.8 μL min -1 . For a batch culture in the reactor droplet, no AC voltage was applied; i.e., the carrier and reactor droplets did not fuse. For a continuous culture in the reactor droplet, 300 V (peak-to-peak; 1 kHz) of an AC voltage was applied with an interval of T; under these conditions, the fusion duration, w, was approximately 1.5 s. The temperature was set at 37°C using a thermoplate (MATS-U52RA26; Tokai Hit, Shizuoka, Japan). Images were taken every 2 min for 72 h using an optical microscope (IX71) and a digital camera (EOS 60D), and then analyzed using an ImageJ. The turbidity was analyzed as described above for the optical measurements. Numerical analyses were performed using MATLAB.
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