Anti dykddddk tag beads

Manufactured by Fujifilm

Sourced in United States

Anti‐DYKDDDDK‐tag beads are affinity purification resin designed for the isolation and purification of DYKDDDDK-tagged proteins from cell lysates or culture supernatants. The beads are composed of agarose support matrix covalently coupled with anti-DYKDDDDK antibody fragments. This product can be used to capture and isolate DYKDDDDK-tagged proteins for further analysis or applications.

Automatically generated - may contain errors

Lab products found in correlation

Phos tag acrylamide, by Fujifilm (2 mentions) Mouse anti gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) Rabbit anti yap taz d24e4, by Cell Signaling Technology (1 mentions) Basic fibroblast growth factor (bfgf), by Fujifilm (1 mentions) Dexamethasone d4902, by Merck Group (1 mentions) Insulin i5500, by Merck Group (1 mentions) Genistein g6649, by Merck Group (1 mentions) Hoechst 33342, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-DYKDDDDK (FLAG) tag antibody beads Anti-DYKDDDDK tag antibody magnetic beads Anti-DYKDDDDK tag antibody beads Anti-DYKDDDDK beads Anti-DYKDDDDK

2 protocols using anti dykddddk tag beads

Antibody Panel for Cellular Signaling Analysis

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The antibodies and the reagents were obtained from commercial sources: mouse anti‐GFP (SC‐9996; Santa Cruz Biotechnology, Dallas, TX, USA); Hoechst 33342 and mouse β‐actin (A1978; Sigma‐Aldrich, St. Louis, MO, USA); anti‐DYKDDDDK‐tag (014‐22383), anti‐DYKDDDDK‐tag beads (016‐22784), and Phos‐tag acrylamide (Wako Pure Chemical Industries, Osaka, Japan); and rabbit anti‐S909/S871 LATS1/2 (#9157S) and rabbit anti‐YAP/TAZ (D24E4) (#8418) (Cell Signaling Technology, Danvers, MA, USA). Rabbit anti‐phospho‐S89 TAZ and anti‐T1079/T1041 LATS1/2 antibodies were described previously.23, 27

Nagashima S., Maruyama J., Kawano S., Iwasa H., Nakagawa K., Ishigami‐Yuasa M., Kagechika H., Nishina H, & Hata Y. (2016). Validation of chemical compound library screening for transcriptional co‐activator with PDZ‐binding motif inhibitors using GFP‐fused transcriptional co‐activator with PDZ‐binding motif. Cancer Science, 107(6), 791-802.

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Antibody and Reagent Database for Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies and reagents were obtained from commercial sources. The antibodies are listed in the supplementary table (S1 Table). Hoechst 33342, dexamethasone (D4902), epidermal growth factor (E9644), insulin (I5500), and genistein (G6649) (Sigma-Aldrich); anti-DYKDDDDK-tag (014–22383), anti-DYKDDDDK-tag beads (016–22784), basic fibroblast growth factor (064–04541) and Phos-tag acrylamide (WAKO chemicals); LY294002 and Akti-1/2 (Tocris Bioscience); wortmannin and rapamycin (Cayman Chemical); recombinant myostatin (788-G8-010) (R&D systems); and human TNF-α (300-01A) (PeproTech).

Kodaka M., Mao F., Arimoto-Matsuzaki K., Kitamura M., Xu X., Yang Z., Nakagawa K., Maruyama J., Ishii K., Akazawa C., Oyaizu T., Yamamoto N., Ishigami-Yuasa M., Tsuemoto N., Ito S., Kagechika H., Nishina H, & Hata Y. (2020). Characterization of a novel compound that promotes myogenesis via Akt and transcriptional co-activator with PDZ-binding motif (TAZ) in mouse C2C12 cells. PLoS ONE, 15(4), e0231265.

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