Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin solution, and 0.25 % trypsin-EDTA were purchased from Invitrogen/Gibco (Grans Island, NY, USA). TNF-α, thiazolyl blue tetrazolium bromide (MTT), and 2',7'dichlorodihydrofluorescein diacetate (H 2 DCFDA) were purchased from Sigma Chemical (St.Louis, MO, USA). Tin protoporphyrin IX (SnPP) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Primary antibodies for CD54/ICAM-1, VCAM-1, HO-1, and glycerol-3-phosphate dehydrogenase (GAPDH) and goat anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Protease inhibitor cocktail tablets, CL-XPosre film, and the Super Signal West Pico Chemiluminescent substrate were obtained from Thermo Scientific (Waltham, MA, USA). Tin protoporphyrin IX (SnPP) was from Cayman Chemical (Ann Arbor, MI, USA).
Goat anti rabbit igg horseradish peroxidase hrp conjugated secondary antibodies
Manufactured by Cell Signaling Technology
Sourced in United States
Goat anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibodies are a laboratory reagent used to detect and quantify rabbit primary antibodies in various immunoassays. The goat-derived antibodies are conjugated to the enzyme horseradish peroxidase, which catalyzes a colorimetric reaction when exposed to an appropriate substrate, allowing for the visualization and measurement of target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Protease inhibitor cocktail tablet, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Tin protoporphyrin 9, by Cayman Chemical (1 mentions)
Tnf α, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
2 7 dichlorodihydrofluorescein diacetate h2dcf da, by Merck Group (1 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Thiazolyl blue tetrazolium bromide mtt, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Pd98059, by New England Biolabs (1 mentions)
Isobutylmethylxanthine, by Merck Group (1 mentions)
Superfect transfection reagent, by Qiagen (1 mentions)
Dexamethasone (dex), by Steraloids (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Insulin, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Indomethacin, by Merck Group (1 mentions)
2 protocols using goat anti rabbit igg horseradish peroxidase hrp conjugated secondary antibodies
1
Modulation of Inflammatory Responses
2
Molecular Signaling Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The LRP16 plasmid was described in our previous reports [12] . LRP16 and Rac1 specific siRNA were chemically synthesized by GenChem Co (Shanghai, China). LPS was purchased from Sigma Aldrich (St. Louis, Missouri, USA). The ERK specific inhibitor, PD98059, was obtained from New England Biolabs. DMEM and fetal bovine serum (FBS) were obtained from Gibco (Paisley, USA). Lipofectamine 2000 was purchased from Invitrogen. SuperFect transfection reagent was obtained from QIAGEN. Dexamethasone was obtained from Steraloids. Insulin, indomethacin, isobutylmethylxanthine, and peroxisome proliferator-activated receptor (PPAR) agonist were purchased from Sigma Aldrich. Rabbit anti-Rac1 antibody was purchased from Sigma Aldrich. Rabbit antibodies against TNFα, c-Jun, c-fos, NF-κB, p65, IL-1β, IL-2, IL-4, IL-6, and phosphorylated (p)-ERK1/2 were purchased from Abcam (Cambridge, Massachusetts, USA). Goat anti-rabbit IgG horse radish peroxidase (HRP)-conjugated secondary antibodies were purchased from Cell Signaling Technology (Beverly, Massachusetts, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!