X gal stock solution

Manufactured by Merck Group

Sourced in Canada

X-Gal stock solution is a laboratory reagent used in molecular biology and microbiology. It is a colorimetric substrate that is commonly used to detect the presence of the enzyme beta-galactosidase, which is often used as a reporter gene or a marker for gene expression.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) D mannitol, by Merck Group (2 mentions) Lactose anhydrous, by Merck Group (1 mentions) D trehalose dihydrate, by Merck Group (1 mentions) L leucine, by Merck Group (1 mentions)

Spelling variants of this product

X-gal (146 citations) X-gal solution (8 citations)

2 protocols using x gal stock solution

Adenoviral Vector Production Protocol

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D-mannitol and dextran (M r 40,000 kDa) were purchased as USP grades from Sigma-Aldrich (Ontario, Canada). Culture media was produced from α-minimum essential medium (prepared in the lab according to protocol by the supplier, Life Technologies; Ontario, Canada) with 10% fetal bovine serum and 1% streptomycin/penicillin (Invitrogen; Ontario, Canada). X-Gal stock solution was purchased from EMD Millipore (Ontario, Canada). A recombinant replication-defective human type 5 adenovirus expressing Escherichia coli β-galactosidase (AdHu5LacZ) was produced in the vector facility of McMaster Immunology Research Centre as described previously [34] .

LeClair D.A., Cranston E.D., Xing Z, & Thompson M.R. (2016). Optimization of Spray Drying Conditions for Yield, Particle Size and Biological Activity of Thermally Stable Viral Vectors. Pharmaceutical research, 33(11).

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Adenoviral Vector Production for β-Galactosidase

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Anhydrous lactose, D-(+)-trehalose dihydrate, D-mannitol, dextran (M r 40000 kDa) and L-leucine were all purchased as USP grades from Sigma-Aldrich (Ontario, Canada). Culture media was produced from α-minimum essential medium (prepared in the lab according to protocol by Life Technologies (Ontario, Canada)) with 10% fetal bovine serum and 1% streptomycin/penicillin (Invitrogen; Ontario, Canada). X-Gal stock solution was purchased from EMD Millipore (Ontario, Canada). A recombinant replication-defective human type 5 adenovirus expressing Escherichia coli β-galactosidase (AdHu5LacZ) was produced in the vector facility of McMaster Immunology Research Centre as described previously (Xing et al., 1996) .

LeClair D.A., Cranston E.D., Xing Z, & Thompson M.R. (2016). Evaluation of excipients for enhanced thermal stabilization of a human type 5 adenoviral vector through spray drying. International journal of pharmaceutics, 506(1-2).

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