Florisil cartridges

Standards of BDE-28, -49, -47, -99, -100, -153, -154, -138, -183, and -209, 1,2-Bis(2,4,6tribromophenoxy)ethane (BTBPE), syn-DP and anti-DP isomers, 2-ethylhexyl-2,3,4,5 tetrabromobenzoate (TBB), 2,3,4,5-tetrabromophthalate (TBPH), hexabromobenzene (HBB), dechlorane-602 (Dec-602), dechlorane-603 (Dec-603), isotopically labelled internal standards (IS) 13 C-BDE-209, 13 C-TBPH, 13 C-TBB, 13 C-syn-DP, and 13 C-anti-DP were purchased from Wellington Laboratories (Guelph, ON, Canada). Recovery standard (RS) CB-207 was purchased from Dr. Ehrenstorfer Laboratories (Augsburg, Germany). Polypropylene (PP) tubes (15 mL) were obtained from Greiner Bio-one (Belgium). Empty PP cartridges (25 mL) were purchased from Grace (Lokeren, Belgium), while Florisil® cartridges (500 mg, 3 mL) and empty PP cartridges (6 mL) were purchased from Supelco (Bellefonte, PA, USA). Silica gel, anhydrous sodium sulphate (Na 2 SO 4 ) and concentrated sulfuric acid (H 2 SO 4 , 98%) were purchased from Merck (Darmstadt, Germany). All solvents were of chromatography grade: n-hexane was purchased from Acros Organics (Belgium); dichloromethane (DCM), iso-octane, toluene and acetonitrile (ACN) were purchased from Merck.

Standards of TCEP, TDCIPP, TEHP, EHDPHP, TCIPP (mixture of 2 isomers), and TPHP were purchased from Chiron AS (Trondheim, Norway). Triamyl phosphate (TAP) (IS) was purchased from TCI Europe (Zwijndrecht, Belgium). Labeled internal standards, TPHP-d15, TDCIPP-d15, TBOEP-d6, and TCEP-d12 (IS) were custom synthesized. Recovery standard (RS) chlorobiphenyl CB-207 was purchased from Dr. Ehrenstorfer Laboratories (Augsburg, Germany). Polypropylene syringes were purchased by Sigma-Aldrich (St. Louis, MO, USA). QuE Z-Sep sorbent powder and Florisil ® cartridges (500 mg, 3 mL) were purchased from Supelco (Bellefonte, PA, USA). All solvents were chromatography grade: n-hexane (n-Hex) was purchased from Acros Organics (Belgium); ethyl acetate (ETAC), dichloromethane (DCM), iso-octane, and acetonitrile (ACN) were purchased from Merck (Darmstadt, Germany). All chemicals were analytical reagent grade or equivalent analytical purity.