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Mab5326

Manufactured by Fortrea
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MAB5326 is a laboratory instrument designed for the detection and quantification of specific biomolecules. It utilizes monoclonal antibody-based technology to provide accurate and reliable results. The core function of this product is to facilitate targeted analysis in a research or diagnostic setting.

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Lab products found in correlation

Ix51 inverted fluorescent microscope, by Olympus (2 mentions) Prb 278p, by Fortrea (2 mentions) Anti map2, by Merck Group (1 mentions) Anti tuj1, by Merck Group (1 mentions) Donkey serum, by Jackson ImmunoResearch (1 mentions) Anti nanog, by Abcam (1 mentions) Anti α sma, by Merck Group (1 mentions) Anti oct3 4, by Santa Cruz Biotechnology (1 mentions) Anti sox2, by Santa Cruz Biotechnology (1 mentions) Anti pax6, by Fortrea (1 mentions)

3 protocols using mab5326

1

Immunostaining of Stem Cell Markers

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Cells were fixed with 4% paraformaldehyde for 30 min at room temperature, washed with PBS, permeabilized in 0.4% Trion X-100 in PBS, and then blocked in 10% donkey serum (Jackson ImmunoResearch Labs). After that, cells were incubated with primary antibodies in blocking solution at 4°C overnight, followed by incubation with corresponding secondary antibodies and Hoechst 33342 for 1 h at room temperature. The primary antibodies used include anti-NANOG (Abcam, 21624), anti-OCT3/4 (Santa Cruz,5279), anti-SOX2 (Santa Cruz,17320), anti-NESTIN (Millipore, MAB5326), anti-PAX6 (Covance, PRB-278P), anti-TUJ1 (Sigma, T2220), anti-MAP2 (Sigma, 4403), anti-FOXA2 (CST, 8186), anti-α-SMA (Sigma, A5228).
Fu L., Xu X., Ren R., Wu J., Zhang W., Yang J., Ren X., Wang S., Zhao Y., Sun L., Yu Y., Wang Z., Yang Z., Yuan Y., Qiao J., Belmonte J.C., Qu J, & Liu G.H. (2016). Modeling xeroderma pigmentosum associated neurological pathologies with patients-derived iPSCs. Protein & Cell, 7(3), 210-221.
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2

Immunofluorescence Staining of Neural Progenitors

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Cells were seeded on coverslips and fixed in 4% PFA or 100% cold methanol, permeabilized with 0.1% Triton-X, and blocked with 0.1% BSA, 0.5% gelatin from cold water fish skin in PBS. Cells were incubated with primary antibody in blocking solution overnight at 4°C, washed and incubated with secondary antibody in blocking solution and 0.4 µg Hoechst for 1 hour at room temperature. Primary antibodies used were mouse anti-Nestin (EMD Millipore, MAB5326) and rabbit anti-Pax6 (Covance, PRB-278P) at 1:1000. Secondary antibodies were Alexa 555 anti-rabbit and Alexa 488 anti-mouse at 1:500. Images were taken with an Olympus IX51 inverted fluorescent microscope.
Lardelli R.M., Schaffer A.E., Eggens V.R., Zaki M.S., Grainger S.L., Sathe S., Van Nostrand E.L., Schlachetzki Z., Rosti B., Akizu N., Scott E., Heckman L.D., Rosti R.O., Dikoglu E., Gregor A., Guemez-Gamboa A., Musaev D., Mande R., Widjaja A., Shaw T.L., Markmiller S., Marin-Valencia I., Davies J.H., de Meirleir L., Kayserili H., Altunoglu U., Freckmann M.L., Warwick L., Chitayat D., Çağlayan A.O., Bilguvar K., Per H., Fagerberg C., Kibaek M., Aldinger K.A., Manchester D., Matsumoto N., Muramatsu K., Saitsu H., Shiina M., Ogata K., Foulds N., Dobyns W.B., Chi N., Traver D., Spaccini L., Bova S.M., Gabriel S.B., Gunel M., Valente E.M., Nassogne M.C., Bennett E.J., Yeo G.W., Baas F., Lykke-Andersen J, & Gleeson J.G. (2017). Biallelic Mutations in the 3’ Exonuclease TOE1 Cause Pontocerebellar Hypoplasia and Uncover a Role in snRNA Processing. Nature genetics, 49(3), 457-464.
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3

Immunofluorescence Staining of Neural Progenitors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded on coverslips and fixed in 4% PFA or 100% cold methanol, permeabilized with 0.1% Triton-X, and blocked with 0.1% BSA, 0.5% gelatin from cold water fish skin in PBS. Cells were incubated with primary antibody in blocking solution overnight at 4°C, washed and incubated with secondary antibody in blocking solution and 0.4 µg Hoechst for 1 hour at room temperature. Primary antibodies used were mouse anti-Nestin (EMD Millipore, MAB5326) and rabbit anti-Pax6 (Covance, PRB-278P) at 1:1000. Secondary antibodies were Alexa 555 anti-rabbit and Alexa 488 anti-mouse at 1:500. Images were taken with an Olympus IX51 inverted fluorescent microscope.
Lardelli R.M., Schaffer A.E., Eggens V.R., Zaki M.S., Grainger S.L., Sathe S., Van Nostrand E.L., Schlachetzki Z., Rosti B., Akizu N., Scott E., Heckman L.D., Rosti R.O., Dikoglu E., Gregor A., Guemez-Gamboa A., Musaev D., Mande R., Widjaja A., Shaw T.L., Markmiller S., Marin-Valencia I., Davies J.H., de Meirleir L., Kayserili H., Altunoglu U., Freckmann M.L., Warwick L., Chitayat D., Çağlayan A.O., Bilguvar K., Per H., Fagerberg C., Kibaek M., Aldinger K.A., Manchester D., Matsumoto N., Muramatsu K., Saitsu H., Shiina M., Ogata K., Foulds N., Dobyns W.B., Chi N., Traver D., Spaccini L., Bova S.M., Gabriel S.B., Gunel M., Valente E.M., Nassogne M.C., Bennett E.J., Yeo G.W., Baas F., Lykke-Andersen J, & Gleeson J.G. (2017). Biallelic Mutations in the 3’ Exonuclease TOE1 Cause Pontocerebellar Hypoplasia and Uncover a Role in snRNA Processing. Nature genetics, 49(3), 457-464.
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