Ml329

Anti-MITF (clone D5G7V) and phospho-p44/42 MAPK antibodies were from Cell Signaling Technology (Danvers, MA, USA). Anti-phospho-MITF (Ser73/180) and mouse anti-αTubulin antibodies were from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-Lamin β1 antibody was purchased from Abcam (Cambridge, UK). PE anti-human FcϵRI antibody was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Anti-LysRS (D-4) and PE anti-human CD117 were obtained from Santa Cruz Biotechnology, Inc (Santa Cruz, CA, USA). PE anti-human MRGPRX2 was from Bio Legend (San Diego, CA, USA ). APC anti-human CD63 and FITC Annexin V were from ImmunoTools GmbH (Friesoythe, Germany). Biotinylated human IgE was from Abbiotec (San Diego, CA, USA), and streptavidin was from Sigma (St. Louis, MO, USA). Substance P was from AnaSpec (Fremont, CA). ML329 was obtained from Axon Med Chem (Groningen, The Netherlands). Morphine was obtained from B. Braun Medical S.A (Spain), the muscle relaxant atracurium was from Pfizer Inc (NY, USA), meglumine amidotrizoate was from Juste Laboratories (Spain), and the vancomycin antibiotic was from Normon (Spain).

Mouse anti-3BP2 (clone C5), mouse anti-KIT (clone Ab81), mouse anti-BCL2, and mouse anti-CDK2 were purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA, USA). Anti-MITF (clone D5G7V) was obtained (was provided by) from Cell Signaling Technology, Inc (Danvers, MA, USA). Mouse anti-β-actin (clone AC-40) and mouse anti-tubulin (DM1A) were purchased from Sigma (St. Louis, MO, USA). Anti-mouse and anti-rabbit IgG peroxidase Abs were acquired and bought from DAKO (Carpinteria, CA, USA) and Biorad (Hercules, CA, USA). ML329 was from Axon Med Chem (Groningen, The Netherlands).