A2780

Cancer cell lines (SKOV3, CAOV3, ES-2, A2780, OVCAR8, HEY, OVCAR429, and OVCAR3) were obtained from the Shanghai Institute of Cancer Research. All cells were cultured in various media: ES-2, CAOV3, A2780, and HEY in DMEM (HyClone Laboratories Inc., Logan, UT, USA); SKOV3, OVCAR8, and OVCAR429 in RPMI 1640 medium (HyClone Laboratories Inc., Logan, UT, USA). OVCAR3 cells were cultured in RPMI 1640 supplemented with 20 % (v/v) FBS (Gibco, New York, USA). All media were supplemented with 10 % (v/v) fetal bovine serum (FBS) and 1 % antibiotics (100 μg/mL streptomycin and 100U/ml penicillin). Cells were all maintained at 37 °C in a humidified incubator with 5 % CO₂.

Cancer cell lines (SKOV3, Coci, CAOV3, A2780, SW626, HEY, and OVCAR3) and the normal ovarian cell line IOSE80 were obtained from the Shanghai Cancer Institute. All cells were cultured in different culture media (SKOV3 in 5A (Gibco, New York, USA); Coci, CAOV3, A2780, SW626, and HEY in DMEM (HyClone Laboratories Inc., Logan, UT, USA); and IOSE80 in RPMI 1640 medium (HyClone Laboratories Inc., Logan, UT, USA)) supplemented with 10% (volume/volume) foetal bovine serum (FBS) (Gibco, New York, USA). OVCAR3 cells were cultured in DMEM (HyClone Laboratories Inc., Logan, UT, USA) supplemented with 20% (volume/volume) FBS. All culture media were supplemented with 1% double antibiotics (100 μg/ml streptomycin and 100 U/ml penicillin). The cells were all cultured in a 37°C humidified incubator supplemented with a 5% CO₂ atmosphere. Recombinant human IL-6 protein (Proteintech, Wuhan, China) was added to the OC cell culture medium after culturing.