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Anti tuj1 mouse igg2a

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Anti-TUJ1 mouse IgG2a is a laboratory reagent used for the detection and analysis of the beta-III tubulin (TUJ1) protein in various biological samples. It is a monoclonal antibody of the IgG2a subclass, derived from mouse. This reagent is typically used in immunohistochemical, immunocytochemical, and Western blot applications to identify and quantify the expression of TUJ1, a marker for neuronal cells.

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Lab products found in correlation

Alexa fluor tagged secondary antibodies, by Thermo Fisher Scientific (2 mentions) Evos fl fluorescence microscope, by Thermo Fisher Scientific (2 mentions) Anti tbr1 rabbit igg, by Abcam (2 mentions) Anti nestin mouse igg1, by Neuromics (2 mentions)

Spelling variants of this product

Mouse anti-Tuj1 (44 citations) Anti-Tuj1 (23 citations) Mouse Tuj1 (5 citations)

2 protocols using anti tuj1 mouse igg2a

1

Immunofluorescence Characterization of CNS Neurons

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Cells were fixed by incubation in 4% paraformaldehyde at room temperature for 15 minutes and washed with PBS. They were permeabilized at room temperature and incubated with PBS supplemented with 0.3% Triton X100, 0.5% BSA, and 1% goat serum for 30 minutes for antigen blocking. The cells were then washed and stained by overnight incubation with primary antibodies at 4°C. The primary antibodies used were: anti-TBR1 rabbit IgG (AbCam, ab31940), anti-TUJ1 mouse IgG2a (Covance, MMS-435P), and anti-Nestin mouse IgG1 (Neuromics, MO15012). The signals were visualized by staining the cells with Alexa Fluor-tagged secondary antibodies (Invitrogen) and DAPI, and imaging under an EVOS FL fluorescence microscope (Thermo Fisher Scientific). For quantification purposes, we used a minimum of three images of CNS neurons, counting the DAPI/Nestin, DAPI/TUJ1, or TUJ1/TBR1 double-positive cells on these images by eye. For each image, we counted a minimum of 100 cells.
Lafaille F.G., Harschnitz O., Lee Y.S., Zhang P., Hasek M.L., Kerner G., Itan Y., Ewaleifoh O., Rapaport F., Carlile T.M., Carter-Timofte M.E., Paquet D., Dobbs K., Zimmer B., Gao D., Rojas-Duran M.F., Kwart D., Rattina V., Ciancanelli M.J., McAlpine J.L., Lorenzo L., Boucherit S., Rozenberg F., Halwani R., Henry B., Amenzoui N., Alsum Z., Marques L., Church J.A., Al-Muhsen S., Tardieu M., Bousfiha A.A., Paludan S.R., Mogensen T.H., Quintana-Murci L., Tessier-Lavigne M., Smith G.A., Notarangelo L.D., Studer L., Gilbert W., Abel L., Casanova J.L, & Zhang S.Y. (2019). Human SNORA31 variations impair cortical neuron-intrinsic immunity to HSV-1 and underlie herpes simplex encephalitis. Nature medicine, 25(12), 1873-1884.
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2

Immunofluorescence Characterization of CNS Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed by incubation in 4% paraformaldehyde at room temperature for 15 minutes and washed with PBS. They were permeabilized at room temperature and incubated with PBS supplemented with 0.3% Triton X100, 0.5% BSA, and 1% goat serum for 30 minutes for antigen blocking. The cells were then washed and stained by overnight incubation with primary antibodies at 4°C. The primary antibodies used were: anti-TBR1 rabbit IgG (AbCam, ab31940), anti-TUJ1 mouse IgG2a (Covance, MMS-435P), and anti-Nestin mouse IgG1 (Neuromics, MO15012). The signals were visualized by staining the cells with Alexa Fluor-tagged secondary antibodies (Invitrogen) and DAPI, and imaging under an EVOS FL fluorescence microscope (Thermo Fisher Scientific). For quantification purposes, we used a minimum of three images of CNS neurons, counting the DAPI/Nestin, DAPI/TUJ1, or TUJ1/TBR1 double-positive cells on these images by eye. For each image, we counted a minimum of 100 cells.
Lafaille F.G., Harschnitz O., Lee Y.S., Zhang P., Hasek M.L., Kerner G., Itan Y., Ewaleifoh O., Rapaport F., Carlile T.M., Carter-Timofte M.E., Paquet D., Dobbs K., Zimmer B., Gao D., Rojas-Duran M.F., Kwart D., Rattina V., Ciancanelli M.J., McAlpine J.L., Lorenzo L., Boucherit S., Rozenberg F., Halwani R., Henry B., Amenzoui N., Alsum Z., Marques L., Church J.A., Al-Muhsen S., Tardieu M., Bousfiha A.A., Paludan S.R., Mogensen T.H., Quintana-Murci L., Tessier-Lavigne M., Smith G.A., Notarangelo L.D., Studer L., Gilbert W., Abel L., Casanova J.L, & Zhang S.Y. (2019). Human SNORA31 variations impair cortical neuron-intrinsic immunity to HSV-1 and underlie herpes simplex encephalitis. Nature medicine, 25(12), 1873-1884.
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