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3 protocols using antibody against β actin

1

Biomimetic Hydrogel for Cellular Uptake

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Lauric acid, bovine serum albumin (BSA), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). Dulbecco’s modified Eagle medium (DMEM) and fetal bovine serum (FBS) were obtained from Gibco BRL (Gaithersburg, MD). N-acryloxysuccinimide (NAS), acrylamide (AAm), N,N-methylene bisacrylamide (BIS), ammonium persulfate (APS), N,N,N′,N′-tetramethylethylenediamine (TEMED) and fluorescein isothiocyanate (FITC) were from Aladdin Industrial Co. Ltd. (Shanghai, China). N-(3-Aminopropyl) methacrylamide hydrochloride was purchased from Polymer Science (Monticello, IN). Lipofectamine 2000 was purchased from Life Technologies (Carlsbad, CA). DAPI and Lyso-Tracker Red were from Beyotime Biotechnology (Shanghai, China), Antibodies against LC3, Arf-6, Flotillin, Cdc42, RhoA, P62, EEA1, clathrin, and caveolin were from Cell Signaling Technology, Inc. (Danvers, MA). Antibody against β-actin was obtained from Abmart, Inc. (Shanghai, China). All other materials were commercially available and used as received.
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2

Cellular Endocytosis Pathway Analysis

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Bovine serum albumin, dimethyl sulphoxide (DMSO), Chloroquine (CQ), and 3-methyladenine (3-MA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-acryloxysuccinimide (NAS), acrylamide (AAm), N, N-methylene bisacrylamide (BIS), ammonium persulphate (APS), N, N, N', N'-tetramethylethylenediamine (TEMED) and Fluorescein isothiocyanate (FITC) were purchased from Aladdin Industrial Co. LTD. (Shanghai, China). N-(3-Aminopropyl) methacrylamide hydrochloride was purchased from Polymer Science, Inc. All other chemicals of the highest quality were commercially available and used as received. Lyso-Tracker Red was purchased from Beyotime Biotechnology (Shanghai, China). Antibodies against LC3, Arf-6, Flotillin, Cdc42, RhoA, P62, EEA1, Clathrin, Caveolin were from Cell Signaling Technology. The antibody against LAMP1 was from Santa Cruz Biotechnologies, and the antibody against β-actin was obtained from Abmart.
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3

Polymer-Based Docetaxel Delivery System

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CQ, 3-MA, stannous octoate (Sn(Oct)2) and D,L-lactide (LA) were purchased from Sigma-Aldrich (St. Louis, MO). Boltorn® H40 (H40, a dendritic polyester with 64 hydroxyl terminal groups per molecule, Mn 2833) was obtained from Perstorp Polyols AB Co., Sweden. Docetaxel was from Shanghai Jinhe Bio-tech Co., Ltd. (Shanghai, China). Acetonitrile and methanol were purchased from EM Science (HPLC grade, Mallinckrodt Baker, USA). All other chemicals of highest quality were commercially available. Antibodies against LC3 and LAMP1 were from Cell Signaling Technology, Inc. (Danvers, MA). Antibody against β-actin was obtained from Abmart, Inc (Shanghai, China).
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