A549 (alveolar basal carcinoma), MCF-7 and MDA-MB-231 (breast adenocarcinoma), BEL-7402 (hepatoma), L929 (murine fibroblasts) and A549/CDDP (cisplatin-resistant lung cancer) human cell lines were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). 3-(4,5-Dimethyl-thinazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and 4,4′-bipyridine were obtained from Sigma-Aldrich. Annexin V-FITC apoptosis detection kit was purchased from Beyotime Biotechnology (Haimen, Jiangsu, China). Fetal bovine serum (FBS) was supplied by Shanghai Ponsure Biotechnology Co., Ltd. (Shanghai, China). RPMI-1640 medium was purchased from Gibco Laboratories (NY, USA). All other chemicals and solvents were commercially available and used without further purification.
A549 cddp
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The A549/CDDP cell line is a cisplatin-resistant variant of the A549 human lung adenocarcinoma cell line. It is commonly used in cancer research and drug development studies.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Bel 7402, by National Collection of Authenticated Cell Cultures (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Beyotime (1 mentions)
Mda mb 231, by National Collection of Authenticated Cell Cultures (1 mentions)
Mcf 7, by National Collection of Authenticated Cell Cultures (1 mentions)
4 4 bipyridine, by Merck Group (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Si trim16, by GenePharma (1 mentions)
2 protocols using a549 cddp
1
Cytotoxicity Evaluation of Cell Lines
2
Exploring CDDP-resistant NSCLC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The NSCLC cell lines (A549 and H1299) and their CDDP-resistant cell lines (A549/CDDP and H1299/CDDP cells), as well as normal lung epithelial cell lines (16HBE), were purchased from the Cell Bank of the Chinese Academy of Sciences. The cell lines were cultured in RPMI 1640 medium (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) in a humidified atmosphere with 5% CO2 at 37°C.
The overexpression of cirPTK2 plasmids (circPTK2) and inhibition TRIM16 plasmids (si-TRIM16#1: 5ʹ-AACCTGCATGGTGAATTACTGTGAA-3ʹ, si-TRIM16#2: 5ʹ-CGCATCAGGTGAACATCAAACTGCA-3ʹ), as well as their negative control (vector or si-con) were constructed and purchased from GenePharma (Shanghai, China). miR-942 inhibitors and mimics (anti-miR-942 and miR-942) were purchased from RiboBio (Guangzhou, China). All plasmids and oligos were transfected into cell lines by using Lipofectamine 3000 (Life-Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions
The overexpression of cirPTK2 plasmids (circPTK2) and inhibition TRIM16 plasmids (si-TRIM16#1: 5ʹ-AACCTGCATGGTGAATTACTGTGAA-3ʹ, si-TRIM16#2: 5ʹ-CGCATCAGGTGAACATCAAACTGCA-3ʹ), as well as their negative control (vector or si-con) were constructed and purchased from GenePharma (Shanghai, China). miR-942 inhibitors and mimics (anti-miR-942 and miR-942) were purchased from RiboBio (Guangzhou, China). All plasmids and oligos were transfected into cell lines by using Lipofectamine 3000 (Life-Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions
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