Secologanoside

A total of 100 batches of LJF and LF samples were collected from all over China, including 77 batches of cultivated Lonicerae japonicae flos (cLJF) with different processing methods and different origins, 10 batches of wild Lonicerae japonicae flos (wLJF), and 13 batches of Lonicerae flos (LF). The detailed sample information is shown in Table 3. All the samples were authenticated by Chief Pharmacist Ji Zhang, who is the former Director of the Traditional Chinese Medicine Herbarium at the China National Institute for Food and Drug Control. Voucher samples were preserved and stored in the cold sample room of Shenzhen Institute for Drug Control.
Deuterated methanol (CD₃OD, purity ≥ 99.8%) containing 0.05% (v/v) TMS was purchased from Merck. Secologanoside (purity 99.28%) analytical standard was acquired from Sichuan Weikeqi Biological Technology Co., Ltd. Secoxyloganin (Chengdu, China) (purity 98.93%) analytical standard was acquired from Shanghai Hongyong Biological Technology Co., Ltd. Macranthoidin A (Shanghai, China) (purity 100.0%) was acquired from Standard Technology Co., Ltd. (Shanghai, China). Macranthoidin B (purity 96.1%), Dipsacoside B (purity 96.8%) and Sweroside (purity 98.3%) were purchased from the National Institute for Food and Drug Control (Beijing, China). All of the other chemicals used in this study were of analytical grade.

A total of 100 batches of LJF and LF samples were collected from all over China, including 77 batches of cultivated Lonicerae japonicae flos (cLJF) with different processing methods and different origins, 10 batches of wild Lonicerae japonicae flos (wLJF), and 13 batches of Lonicerae flos (LF). The detailed sample information is shown in Table 3. All the samples were authenticated by Chief Pharmacist Ji Zhang, who is the former Director of the Traditional Chinese Medicine Herbarium at the China National Institute for Food and Drug Control. Voucher samples were preserved and stored in the cold sample room of Shenzhen Institute for Drug Control.
Deuterated methanol (CD₃OD, purity ≥ 99.8%) containing 0.05% (v/v) TMS was purchased from Merck. Secologanoside (purity 99.28%) analytical standard was acquired from Sichuan Weikeqi Biological Technology Co., Ltd. Secoxyloganin (Chengdu, China) (purity 98.93%) analytical standard was acquired from Shanghai Hongyong Biological Technology Co., Ltd. Macranthoidin A (Shanghai, China) (purity 100.0%) was acquired from Standard Technology Co., Ltd. (Shanghai, China). Macranthoidin B (purity 96.1%), Dipsacoside B (purity 96.8%) and Sweroside (purity 98.3%) were purchased from the National Institute for Food and Drug Control (Beijing, China). All of the other chemicals used in this study were of analytical grade.