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Atrx hpa001906

Manufactured by Merck Group
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Sourced in Germany, United States

The ATRX (HPA001906) is a laboratory equipment product manufactured by Merck Group. It is a protein that serves a core function in cellular processes, but a detailed description of its intended use cannot be provided in an unbiased and factual manner without extrapolation.

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Lab products found in correlation

P53 clone do 7, by Agilent Technologies (2 mentions) Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions) H3k27m, by Merck Group (1 mentions) Idh1 r132h clone h09, by Dianova (1 mentions) Benchmark xt autostainer, by Roche (1 mentions) Idh1 r132h mutant protein clone h09, by Dianova (1 mentions) Ultraview universal dab detection, by Roche (1 mentions) Ventana benchmark xt, by Roche (1 mentions) Rotating microtome, by Leica (1 mentions) Superfrost plus microslides, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

HPA001906 (25 citations)

3 protocols using atrx hpa001906

1

Immunohistochemical Profiling of Brain Tumor Markers

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Sections for immunohistochemical staining were made using a Leica Bond automated staining processor using antibodies against IDH1 R132H (clone H09, 1:500 dilution; DiaNova, Germany), ATRX (HPA001906, 1:100; Sigma Aldrich, St. Louis, MO, USA), p53 (clone DO-7, 1:100 dilution; Dako, Glostrup, Denmark), H3K27M (ABE419, 1:1000 dilution; Millipore, Billerica, MA, USA), Olig-2 (AB9610, 1:250 dilution; Millipore), Glial fibrillary acidic protein (polyclonal, 1:1000 dilution; Dako, USA), and Ki-67 (MIB-1, 1:50 dilution; Labvision, USA). The standard for judging IDH1 R132H, H3K27M, ATRX, and p53 staining was the same as in a previous study.[8 (link)]
Wang L.M., Li Z., Piao Y.S., Cai Y.N., Zhang L.Y., Ge H.J., Xu W.W, & Lu D.H. (2019). Clinico-neuropathological features of isocitrate dehydrogenase 2 gene mutations in lower-grade gliomas. Chinese Medical Journal, 132(24), 2920-2926.
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2

Immunohistochemistry and FISH Analysis

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Immunohistochemistry was performed on formalin-fixed, paraffin embedded tissue sections at the UCSF Immunohistochemistry Laboratory and the UCSF Neuropathology BTRC Biomarkers Laboratory. Primary antibodies used were as follows: histone H3-K27M mutant protein (ABE419, EMD Millipore, Billerica, MA, 1:500 dilution), IDH1-R132H mutant protein (clone H09, DiaNova, Germany, 1:500 dilution), ATRX (HPA001906, Sigma, St Louis, MO, 1:100 dilution), p53 (clone DO-7, Dako, Glostrup, Denmark, 1:00 dilution), BRAF-V600E mutant protein (clone VE1, Ventana, Tucson, AZ). All staining was performed in Ventana or Leica Bond automated staining processors. Specifically, the histone H3-K27M immunostaining was run in a Ventana Benchmark XT autostainer using CC1 antigen retrieval buffer for 30 minutes at 95°C, incubation with 1:500 dilution of primary antibody for 32 minutes, and Ventana ultraView Universal DAB detection. Dual-color FISH for EGFR and CEP7 or PTEN and CEP10 was performed on 5-micron thick FFPE whole sections as previously described (17 (link)).
Solomon D.A., Wood M.D., Tihan T., Bollen A.W., Gupta N., Phillips J.J, & Perry A. (2015). Diffuse Midline Gliomas with Histone H3‐K27M Mutation: A Series of 47 Cases Assessing the Spectrum of Morphologic Variation and Associated Genetic Alterations. Brain Pathology, 26(5), 569-580.
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3

Immunohistochemical Analysis of G3 NETs

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Paraffin-embedded surgical or biopsy specimens of G3 neuroendocrine tumors were sliced with a rotating microtome (Leica Biosystems, Wetzlar, Germany) into 5 μM thick sections and mounted on SuperFrost Plus microslides (Thermo Fisher Scientific, Waltman, MA, USA). Immunolabeling reactions were carried out on a VENTANA BenchMark XT (Ventana Medical Systems Inc., Tucson, AZ, USA) automated slide strainer. The following antibodies were used according to the manufacturer's instructions: DAXX (HPA008736) (Sigma-Aldrich, St. Louis, MO, USA) 1 : 75, one hour at room temperature (RT); ATRX (HPA001906) (Sigma-Aldrich) 1 : 400, one hour at RT; and RB1 (Cell Signaling Technology, Beverly, Massachusetts, USA) 1 : 1000, one hour at RT. The stained sections were analyzed in blind by an expert pathologist in neuroendocrine neoplasms.
Liverani C., Bongiovanni A., Mercatali L., Foca F., Pieri F., De Vita A., Spadazzi C., Miserocchi G., Recine F., Riva N., Nicolini S., Severi S., Martinelli G, & Ibrahim T. (2018). Grading of Neuroendocrine Carcinomas: Correlation of 68Ga-PET/CT Scan with Tissue Biomarkers. Disease Markers, 2018, 6878409.
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