L α phosphatidylinositol

Manufactured by Merck Group

Sourced in United States

L-α-phosphatidylinositol is a phospholipid compound found in cell membranes. It serves as a precursor for the production of various signaling molecules within cells.

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L-phosphatidylinositol (2 citations) Phosphatidylinositol (14 citations)

6 protocols using l α phosphatidylinositol

Lipid Composition Analysis Protocol

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FA-free BSA, (S,S)-bis(monoacylglycero)phosphate (BMP), β-carotene, cholesterol, retinol (ROH), retinyl palmitate (RP), retinyl acetate, triolein, cholesteryl oleate, l-α-phosphatidylinositol, and 1,2-dioleoyl-sn-glycero-3-phosphocholine were purchased from Sigma. Lalistat 2 was a kind gift from Dr. Paul Helquist (Dept. of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN).

Pajed L., Wagner C., Taschler U., Schreiber R., Kolleritsch S., Fawzy N., Pototschnig I., Schoiswohl G., Pusch L.M., Wieser B.I., Vesely P., Hoefler G., Eichmann T.O., Zimmermann R, & Lass A. (2019). Hepatocyte-specific deletion of lysosomal acid lipase leads to cholesteryl ester but not triglyceride or retinyl ester accumulation. The Journal of Biological Chemistry, 294(23), 9118-9133.

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Lipid Synthesis and Characterization

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Soybean-phosphatidylcholine (PC, 95%) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Tributyrin (TC4, 99%), Tricaprylin (TC8, 99%), Triolein (TC18, 99%), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, ≥97%), l-α-phosphatidylinositol (PI, ≥99%), 1,2-diacyl-sn-glycero-3-phospho-l-serine (PS, ≥97%), l-α-phosphatidylethanolamine (PE, ≥99%), cardiolipin (CL, ≥98%), 3-sn-Phosphatidic acid sodium salt (PA, ≥98%), Sphingomyelin (SM, ≥98%), 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (PG, ≥98%), 1,2-distearoyimonoglactosylglyceride (MGDG, ≥98%) were all from Sigma-Aldrich Corporation (St. Louis, MO, USA). Olive oil and sucrose esters were purchased from Aladdin Reagent Co. (Shanghai, China). Six pure chiral pseudoglycerides (didecanoyl-deoxyamino-O-methyl glycerol, DDGs) used in the present studies were synthesized according to the method reported before [23 (link)].

Wang F., Zhang H., Zhao Z., Wei R., Yang B, & Wang Y. (2017). Recombinant Lipase from Gibberella zeae Exhibits Broad Substrate Specificity: A Comparative Study on Emulsified and Monomolecular Substrate. International Journal of Molecular Sciences, 18(7), 1535.

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Lipid-binding Analysis in SMMC-7721 Cells

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The assay was according to the lipid-binding analysis method by Munnik and Wierzchowiecka (2013) (link). Ceremides, ganglioside GM3, galactocerebrosides, sulfatide, D-sphingosine, L-α-phosphatidylinositol and L-phosphatidylinositol (Sigma-Aldrich) were suspended in resuspension buffer (CHCl3/MeOH/H₂O=20:9:1, v/v), and diluted to gradient concentration by spotting buffer (CHCl3/MeOH/50 mM HCl/Ponceau S=250:500:200:2, v/v). The lipids were spotted on PVDF membrane strips and dried at room temperature in the dark. The membranes were blocked in fat blot buffer (50 mM Tris–HCl, 150 mM NaCl, pH 7.5) containing 3% fatty acid-free BSA for 1 h at room temperature, and incubated with SMMC-7721 cell lysates (protein 5 μg/ml) in blocking buffer-0.1% Tween overnight at 4°C. After three washes with fat blot buffer-0.1% Tween, the membranes were incubated with anti-SIN3B antibody and goat anti-rabbit IgG HRP secondary antibody (Jackson ImmunoReseach Lab, Inc.). The blots were visualized using Immunobilon Western (Millipore).

Cai Q., Liu Y., Zhu P., Kang C., Xu H., Qi B., Wang R., Dong Y, & Wu X.Z. (2018). SIN3B promotes integrin αV subunit gene transcription and cell migration of hepatocellular carcinoma. Journal of Molecular Cell Biology, 11(5), 421-432.

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Comprehensive Lipid Extraction and Analysis Protocol

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Methanol, isooctane, acetic acid, butylated hydroxytoluene,
sodium
chloride, pentane, diethyl ether, hexane, and boron trifluoride (BF₃) in methanol are purchased from Merck Millipore, Schiphol-Rijk,
Netherlands. PC-D₇₀ 1,2-distearoyl-D₇₀-sn-glycero-3-phosphocholine was obtained from Avanti Polar
Lipids, Alabaster, Alabama, United States. A phospholipid mixture
for HPLC from soybean containing l-α-lysophosphatidylcholine
(7.7 weight(w)%), l-α-phosphatidylcholine from soybean
(38.5 w%), l-α-phosphatidylethanolamine (30.8 w%),
and l-α-phosphatidylinositol (23.0 w%) were purchased
from Sigma-Aldrich, St. Louis, Missouri, United States. A detailed
list of included FA with their systematic names and a list with FAMEs
and their suppliers is reported in Table 1 and Supplemental Tables S1 and S2.

Schött H.F., Konings M.C., Schrauwen-Hinderling V.B., Mensink R.P, & Plat J. (2021). A Validated Method for Quantification of Fatty Acids Incorporated in Human Plasma Phospholipids by Gas Chromatography–Triple Quadrupole Mass Spectrometry. ACS Omega, 6(2), 1129-1137.

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Lipid Metabolism Regulation in Hepatic Stellate Cells

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Essentially fatty acid (FA)-free bovine serum albumin (BSA), ROH, RP,
retinyl acetate, triolein, L-α-phosphatidylinositol,
1,2-dioleoyl-snglycero-3-phosphocholine, and Orlistat were purchased from Sigma
Aldrich (St. Louis, MO). Atglistatin^®, Lalistat2, and the HSL
inhibitor NNC 0076-0000-0079 (76-0079) were kind gifts from Dr. Rolf Breinbauer
(Institute of Organic Chemistry, University of Technology, Graz, Austria), Dr.
Paul Helquist (Department of Chemistry and Biochemistry, University of Notre
Dame, Notre Dame, IN), and Dr. Christian Fledelius (Novo Nordisk A/S, Novo
Nordisk Park, DK-2706 Måløv, Denmark), respectively.
(R)-Bromoenol lactone (R-BEL) was purchased from Cayman
Chemicals (Ann Arbor, MI). The human HSC line LX-2 was provided by Thierry
Claudel, PhD (Hans Popper Laboratory of Molecular Hepatology, Division of
Gastroenterology and Hepatology, Department of Medicine III, Medical University
of Vienna, Vienna, Austria) with the kind approval of Dr. Scott L. Friedman
(Icahn School of Medicine at Mount Sinai, New York, USA).

Wagner C., Hois V., Pajed L., Pusch L.M., Wolinski H., Trauner M., Zimmermann R., Taschler U, & Lass A. (2020). Lysosomal acid lipase is the major acid retinyl ester hydrolase in cultured human hepatic stellate cells but not essential for retinyl ester degradation. Biochimica et biophysica acta. Molecular and cell biology of lipids, 1865(8), 158730.

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Lipid Metabolism Regulation in Hepatic Stellate Cells

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