Goat polyclonal anti notch1

Rat brains were fixed, embedded in paraffin overnight at 4°C, then cut into 6-μm-thick sections. The sections were deparaffinized, rehydrated, and rinsed with PBS, followed by antigen retrieval in citrate buffer (pH 6.0) for 15 min at 95–100°C. Subsequently, non-specific antigens were blocked in 5% donkey serum for 60 min at 25°C. Then, sections were incubated with primary antibodies as follow: (1) goat polyclonal anti-Notch1 (Santa Cruz Biotechnology, Santa Cruz, CA; 1: 100); (2) goat polyclonal anti-Jagged1 (Santa Cruz Biotechnology; 1: 100); (3) rabbit polyclonal anti-Hes-1 (1: 800 dilution; Abcam, Cambridge, MA, USA); (4) goat polyclonal anti-DCX (1: 200 dilution; Abcam, Cambridge, MA, USA); After incubation, the sections were stained with the nuclear stain 4′,6-diamidino-2-phenyl-indole (DAPI). A fluorescence microscope (Nikon, Japan) was used to observe, evaluate, and photograph these sections.