Morphological investigation of bare and functionalized Ag NPs was performed with transmission electron microscopy (TEM) using a Tecnai microscope (120 kV) and 4k × 4k Eagle camera (Thermofisher, USA). A Zeiss Supra 35 scanning electron microscope (SEM) with field-emission gun (FEG) equipped with scanning transmission electron microscopy (STEM) detector was employed to characterize the distribution of nanoparticles dispersed on copper grid with holely carbon film. The same samples were observed again in Jeol 2100plus transmission electron microscope (TEM) operated at 200 kV to observe the details of individual nanoparticle using double-tilt sample holder and Gatan Rio16 camera with 4k × 4k resolution. The extinction spectra of Ag colloidal solutions were recorded by UV-vis spectroscopy (Shimadzu 2700 UV-vis spectrometer). The hydrodynamic size were measured by dynamic light scattering (DLS) (CORDOUAN Visco Kin). The surface tension and viscosity of the SERS inks were studied by AquaPi portable tensiometer and viscometer DV-II (BROOKFIELD), respectively. The Raman spectra and images were obtained using a Horiba XploRA PLUS Raman microscope equipped with a 638 nm laser.
2700 uv vis spectrometer
Manufactured by Shimadzu
The Shimadzu 2700 UV-vis spectrometer is a compact and versatile instrument designed for accurate UV-vis spectroscopic analysis. It features a wide wavelength range, high-resolution optics, and a robust design for reliable performance in laboratory settings.
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2 protocols using 2700 uv vis spectrometer
1
Characterization of Functionalized Silver Nanoparticles
2
Characterization of Silver Nanoparticles
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The morphology and size distribution of Ag NPs were investigated by TEM images, which were recorded by Tecnai microscope (120 kV) and 4k × 4k Eagle camera (Thermofisher, USA). All the carbon grids were primarily subjected to glow discharge treatment, followed by deposition of 4 µL of sample and incubation under room temperature for 1 min. Then 50 µL of uranyl acetate (2%) was added on the grids before TEM imaging. The extinction spectra of Ag NPs and stability of the colloidal solutions were studied by UV-vis spectroscopy (Shimadzu 2700 UV-vis spectrometer). The SERS spectra and Raman images of cells were recorded using a Horiba XploRA PLUS Raman microscope with 638 nm laser and 2 s exposition time. Hydrodynamic diameters (D H ) were determined by dynamic light scattering (DLS) using a Vasco-Flex particle size analyzer (Cordouan Technologies).
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