The predicted amino acid sequences of the C-terminal fragment of PfMSP-119 from the PCR sequenced genomic fragments were aligned with the corresponding sequence of the P. falciparum genome reference strain 3D7 using the MUSCLE Sequence Alignment algorithms as above. The extent of genetic diversity was estimated by the number and ratio of MSP-119 haplotypes prevalent in the populations. Divergences in the distribution pattern of PfMSP-119 haplotypes between two parasite populations was tested for using the Wright’s fixation index (Fst) with Arlequin suite version 3.5 [64 (link)]. In addition, the departure from the predictions of the neutral mode of molecular evolution was evaluated using three neutrality tests (Tajima’s D, Fu & Li’s D*and Fu & Li’s F*indices) implement in the DnaSP 5.1 program [65 (link)]. Results were deemed to be statistically significant if the p value was less than 0.05 (p < 0.05).
Suite version 3
Manufactured by Arlequin
The Arlequin suite version 3.5 is a software package designed for genetic analysis and population genetics. It provides tools for calculating various genetic parameters and performing statistical analyses on genetic data.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using suite version 3
1
Genetic Diversity Analysis of Pf-MSP1_19
2
Genetic Diversity Analysis of P. falciparum AMA-1
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The P. falciparum AMA-1 sequences from natural isolates in Asia, Oceania, Africa and South America were downloaded from the NCBI database (Supplementary Table S2 ). Nucleotide sequences corresponding to domain I from positions 445–906, corresponding to amino acid residues 149–302, were included in the population differentiation analysis. Arlequin suite version 3.5 software was used to calculate Wright’s fixation index (Fst) to evaluate the differences in the distribution patterns (ratios) of AMA-1 haplotypes between different geographic populations of P. falciparum [58 (link)]. Statistical significance was accepted at a P-value of <0.05.
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