Lysates of cells and tissues were prepared in SDS sample buffer (60 mM Tris-HCl at pH 6.8, 10% glycerol, 2% SDS, and 5% 2-mercaptoethanol). Equal amounts proteins (25 μg) were separated on 4% to 12% SDS-PAGE gels, transferred to PVDF membranes (Invitrogen), and incubated with primary antibodies specific to ERK (Cell Signaling; 4695), P-ERK (Cell Signaling; 4370S), H-RAS (Santa Cruz; sc-520), Krt10 (Covance; PRB-159P), Vinculin (Sigma; V4139). Blots were rinsed in TBST and incubated in peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies, respectively. Proteins were visualized using an ECL detection system (Pierce Biotech) and exposed to film. All experiments were repeated in triplicate.
Prb 159p
Manufactured by Merck Group
The PRB-159P is a laboratory centrifuge designed for a variety of applications. It features a compact and durable construction, allowing for efficient sample separation and processing. The centrifuge can accommodate a range of rotor sizes and sample volumes, making it a versatile tool for various research and diagnostic tasks. Its precise speed and time controls enable users to achieve consistent and reliable results.
Automatically generated - may contain errors
2 protocols using prb 159p
1
Western Blot Analysis of Protein Expression
2
Western Blot Analysis of Protein Expression
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Lysates of cells and tissues were prepared in SDS sample buffer (60 mM Tris-HCl at pH 6.8, 10% glycerol, 2% SDS, and 5% 2-mercaptoethanol). Equal amounts proteins (25 μg) were separated on 4% to 12% SDS-PAGE gels, transferred to PVDF membranes (Invitrogen), and incubated with primary antibodies specific to ERK (Cell Signaling; 4695), P-ERK (Cell Signaling; 4370S), H-RAS (Santa Cruz; sc-520), Krt10 (Covance; PRB-159P), Vinculin (Sigma; V4139). Blots were rinsed in TBST and incubated in peroxidase-conjugated anti-mouse or anti-rabbit secondary antibodies, respectively. Proteins were visualized using an ECL detection system (Pierce Biotech) and exposed to film. All experiments were repeated in triplicate.
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