After contrast-enhanced ultrasonography at the indicated time points (normal group, 1- to 5-week groups after modeling) (Figure 2 ), 2 mL blood was drawn from the medial canthus vein of each rat, and the serum was separated and stored at −80 ℃ for later use. The level of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) clinically used to evaluate hepatic aGVHD was detected by a Mindray® automated hematology analyzer (BS-350E, Shenzhen Mindray Bio-Medical Electronics Co., Ltd, Shenzhen, China).
Bs 350e
Manufactured by Mindray
Sourced in China
The BS-350E is a fully automated clinical chemistry analyzer designed for high-throughput laboratory testing. It features a compact design and offers a wide range of test parameters, ensuring efficient and reliable sample processing.
Automatically generated - may contain errors
14 protocols using bs 350e
1
Serum Liver Enzyme Evaluation in Rat Model
2
Serum Lipid Profile Analysis in Mice
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After 12 h of fasting, mice were anesthetized, and blood samples were collected via cardiac puncture. Serum lipid profiles, including total cholesterol (TC), total triglyceride (TG), low‐density lipoprotein cholesterol (LDL‐C), and high‐density lipoprotein cholesterol (HDL‐C) were measured by enzymatic assay with an automatic biochemical analyzer (Mindray, BS‐350E), respectively.
3
Serum Biochemical Analysis in Mice
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Mouse peripheral blood was collected and centrifuged to obtain serum for analysis. We used a Mindray automated biochemical analyser (BS-350E) to test the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), TG and total cholesterol (TC) levels.
4
Serum Protein Analysis in Mice
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Blood was collected from the eyes of the mice and left to stand for 30 min, after that it was centrifuged at 4°C at 1000 g for 10 min. After the serum was collected, the TP and ALB concentrations were analyzed using an automatic biochemical analyzer (BS‐350E, Shenzhen Mindray Bio‐medical Electronics Co., Ltd.).
5
Measuring Serum Lipid Levels
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Serum levels of TG, TC, and LDL-C were measured by the certified core clinical laboratory at the No. 3 Hospital of Beijing University, who used the respective commercial kits and Chemistry Analyzer (BS-350E) provided by Shenzhen Mindray Biomedical Electronics Co., Ltd. (Shenzhen, China) [75 (link)].
6
Routine Blood Analysis in Mice
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For blood routine examination, 50 μL fresh blood was collected from each mouse and mixed with EDTA immediately. The blood samples were analyzed by Celltac Alpha MEK-6400 series hematology analyzers (Nihon Kohden, MEK-6400). For serum biochemical analysis, blood samples were collected, clotted for 2 h at room temperature or overnight at 4 °C, and then centrifuged (1000× g, 10 min) to obtain serum. 200 μL serum was aliquot and analyzed of Alanine transaminase (ALT), aspartate transaminase (AST), uric acid (UA) and creatinine (CREA) by Chemistry Analyzer (Mindray, BS-350E).
7
Vaccine Safety Evaluation in Mice
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For vaccine safety evaluation, C‐CPS was subcutaneously injected into healthy BALB/c mice with 20 μg of polysaccharide. After 1 h, the mouse body weight and rectal temperature were measured at different time points, and the sera were collected at the same time. Cytokines (IL‐1β, TNF‐α, IL‐6 and IFN‐γ) were detected using commercially available ELISA kits (Dakewe, Shenzhen, China) according to the manufacturer’s instructions. The serum levels of alanine aminotransferase, aspartate transaminase, alkaline phosphatase, lactate dehydrogenase and blood urea nitrogen were detected using a biochemical autoanalyser (BS‐350E, Mindray).
8
Hematological Examination of Drug Toxicity
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Hematological examination was performed to explore the potential toxicity of drugs in vivo. BALB/c nude mice were divided into four groups (n = 5), and injected by NS, naked siRNA (0.3 mg/kg), GCE/siRNA (containing 0.3 mg/kg siRNA), and DOX (1.2 mg/kg) every other day for 5 times. After the drug administration, blood samples were collected from all nude mice, and blood routine test was performed by an automatic hematology analyzer (MEK6400, Nihon Kohden, Japan).
The whole blood was taken from the blood collection vessel without anticoagulant for blood biochemical examination. The whole blood was kept at room temperature for 2 h, and centrifuged (3 000 × g, 4 °C) for 10 min to obtain the upper serum. The important blood biochemical indicators of liver function (ALT: alanine aminotransferase, AST: aspartate aminotransferase, ALP: alkaline phosphatase, TB: total protein, and ALB: albumin) and renal function (CREA: creatinine, UA: uric acid, and UREA) of nude mice were detected by an automatic biochemical analyzer (BS-350E, Mindray, China).
The whole blood was taken from the blood collection vessel without anticoagulant for blood biochemical examination. The whole blood was kept at room temperature for 2 h, and centrifuged (3 000 × g, 4 °C) for 10 min to obtain the upper serum. The important blood biochemical indicators of liver function (ALT: alanine aminotransferase, AST: aspartate aminotransferase, ALP: alkaline phosphatase, TB: total protein, and ALB: albumin) and renal function (CREA: creatinine, UA: uric acid, and UREA) of nude mice were detected by an automatic biochemical analyzer (BS-350E, Mindray, China).
9
Serum Glucose Measurement in Fasted Mice
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Blood samples were collected by cardiac puncture from overnight-fasted mice 48 h after the last exercise session. After clotting, the serum was separated by centrifugation 3500 rpm for 15 min at 4 °C. Serum glucose was measured by the automatic biochemical analyzer [69 (link)] (Mindray, BS-350E) using the specific assay kits (Mindray Co., Ltd., Shenzhen, China).
10
Automated Analysis of Blood Parameters
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The blood samples were analyzed by a technician who was blinded to the experimental grouping using an automated hematology analyzer (MEK-6410C; Nihon Kohden Corporation, Tokyo, Japan). Serum was separated by centrifugation at 10,000 rpm for 5 min. Serum iron levels were measured with an automated biochemistry automatic analyzer (BS-350E; Mindray Medical International Limited, Shenzhen, China). Enzyme-linked immunosorbent assay kits were used to measure serum concentrations of IL-6 (#KE1418; ImmunoWay Biotechnology Company), hepcidin (#KE1286; ImmunoWay Biotechnology Company), ferritin (#KE1279; ImmunoWay Biotechnology Company), and transferrin (#ELM-TF; RayBiotech, Peachtree Corners, GA, U.S.A.) in accordance with the manufacturers' instructions.
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