Agilent 6120 quadrupole lc ms spectrometer

All solvents and reagents for the synthesis were purchased from Sigma-Aldrich Corporation or Fisher Scientific and used without further purification. NMR spectra were recorded on Bruker UltraShield Plus 500 MHz instruments at 24 °C in the designated solvents. Chemical shifts are expressed in ppm relative to TMS (¹H, 0 ppm) or solvent signals: CD₂ Cl₂ (¹H, 5.32 ppm; ¹³C, 53.5 ppm) or THF-d₈ (¹H, 3.58 ppm; ¹³C, 67.21 ppm); coupling constants are expressed in hertz (Hz). High performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis was performed on an Agilent 6120 Quadrupole LC/MS spectrometer (Agilent Technologies). Biochemical reagents and cell culture media were purchased from Fisher Scientific or Sigma-Aldrich Corporation unless otherwise stated. Western blots and gels were imaged on an Odyssey CLx Imaging System (Li-Cor).

All reagents were purchased form Fisher Scientific of Sigma-Aldrich unless otherwise stated. Size Exclusion chromatography was performed on an AKTA FPLC system from GE Healthcare equipped with a P-920 pump and UPC-900 monitor. Preparative purifications were conducted on an Agilent LC system on a C18 preparative column (15–20 um, 20×50 mm, flow rate 20 mL/ minute) or semi-preparative column (12 um, 10×250 mm, flow rate 2 mL/ minute) employing 0.1% TFA in water (Buffer A) and 90% acetonitrile, 0.1% TFA in water (Buffer B) as the mobile phases. HPLC Electrospray ionization MS was performed on an Agilent 6120 Quadrupole LC/MS spectrometer. Concentrations of DNA and proteins were measured spectroscopically with a NanoDrop 2000c (Thermo Scientific). Western blots were imaged on an Odyssey CLx Imaging System (Li-Cor).