FBS, MTT, doxorubicin, anti‐actin antibody, and cell culture medium were purchased from Sigma Chemical Co. (St Louis, MO, USA). QuantiTect SYBR Green PCR Kit was obtained from Qiagen (Germantown, MD, USA). Antibodies against to E‐cadherin, CDH2, vimentin, ZEB1 and β‐catenin were obtained from Cell Signaling Technologies (Danvers, MA, USA). A Dual‐Luciferase Assay Kit, caspase‐3/7 activity assay kit and Lipofectamine 2000 were obtained from Promega (Madison, WI, USA). TRIzol and a BLOCK‐iT™ Pol II miR RNAi Expression Vector Kit were purchased from Invitrogen (Carlsbad, CA, USA). Opti‐MEM, a High‐Capacity cDNA Reverse Transcription Kit, a miRNA expression reporter vector, miR‐708‐3p mimics, antisense oligonucleotides of miR‐708‐3p (ASO miR‐708‐3p), negative control oligonucleotides (NC), and primer sets of RNU6 and miR‐708‐3p were purchased from Life Technologies (Carlsbad, CA, USA). Invasion Assay Kits were obtained from BD Biosciences (Franklin Lakes, NJ, USA). In Situ Cell Death Detection kits were purchased from Roche (Penzberg, Germany).
Caspase 3 7 activity assay kit
Manufactured by Promega
Sourced in United States
The Caspase 3/7 activity assay kit is a laboratory tool used to measure the activity of caspase-3 and caspase-7, two key enzymes involved in the process of apoptosis, or programmed cell death. The kit provides a fluorometric or colorimetric readout that can be used to quantify the degree of caspase-3/7 activation in cell samples.
Automatically generated - may contain errors
Lab products found in correlation
In situ cell death detection kit, by Roche (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Block it pol 2 mir rnai expression vector kit, by Thermo Fisher Scientific (1 mentions)
Ahigh capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Promega (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Anti actin antibody, by Merck Group (1 mentions)
Dual luciferase assay kit, by Promega (1 mentions)
Quantitect sybr green pcr kit, by Qiagen (1 mentions)
Annexin 5 apc flow cytometry assay kit, by BD (1 mentions)
Rneasy mini kit for rna extraction, by Qiagen (1 mentions)
Rna extraction kit, by Qiagen (1 mentions)
Gapdh primer, by Qiagen (1 mentions)
Rt2 profilertm pcr array, by Qiagen (1 mentions)
Cdna kit, by Thermo Fisher Scientific (1 mentions)
8 protocols using caspase 3 7 activity assay kit
1
Molecular Mechanisms of miR-708-3p in Cancer
2
Apoptosis Induction in Ovarian Cancer Cells
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Stable SKOV3 and OVCAR3 cancer cell lines established with lentiviral BRIC5 gRNA and control vectors were treated with the chemotherapy drug paclitaxel at different doses (0, 20, 40 nM) for 24 h. Apoptosis was measured using a caspase3/7 activity assay kit (Promega, Madison, WI). Cell apoptosis was also detected in both SKOV3 and OVCAR3 cells transduced with lentiviral CRISPR/Cas9 nickase-mediated BIRC5 gRNAs and control vectors by using Western blot by detecting cleaved PARP and active caspase 3.
3
Apoptosis Induction in Medulloblastoma Cell Lines
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The ability of inhibitors to induce apoptosis in MB cell lines was determined using an Annexin-V:APC flow cytometry assay kit (BD Biosciences, CA) following the manufacturer's instructions. Briefly, 0.3 × 106 cells/ml MB cell lines were plated in 12-well plates and treated with inhibitors alone or in combination for 72 hours. The percent of the cells undergoing apoptosis was then assessed using Annexin-V/propidium iodide double staining. In some experiments, the induction of apoptosis by inhibitors was also determined using Caspase 3/7 activity assay kit (Promega, WI) following the manufacturer's instructions.
4
Caspase-3/7 Activity Assay Protocol
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The Caspase-3/7 activity assay kit was purchased from Promega (Madison, WI, USA) and used as previously described [42 (link)]. In this assay, the proluminescent substrate containing the amino-acid sequence Asp–Glu–Val–Asp (DEVD) is cleaved by activated caspase 3/7, leading to the release of a luciferase substrate (aminoluciferin) and the generation of a luminescent signal. Data were presented as relative luminescent units (RLU), which were normalized to the corresponding values of control cells as an indicator of caspase-3/7 activities.
5
RNA Extraction and Quantification Protocol
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A Qiagen RNeasy Mini kit for RNA extraction was purchased from Qiagen Sciences (Germantown, MD, USA), and the Super Script (R) First-Strand Synthesis system for cDNA synthesis was purchased from Invitrogen Life Technologies, Carlsbad, CA, USA). The PAF-R and GAPDH primers and the SYBR Green polymerase chain reaction (PCR) reagents were purchased from SABiosciences (Valencia, CA, USA). A caspase-3/7 activity assay kit was purchased from Promega Corporation (Madison, WI, USA). The WEB2086 PAF-R antagonist, was purchased from Cayman Chemicals Co. (Ann Arbor, MI, USA). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
6
Molecular Mechanisms of Stem Cell Regulation
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All chemicals were obtained from Sigma-Aldrich (St. Louis, MO) unless indicated otherwise. The Qiagen RNA extraction kit and RT2 ProfilerTM PCR array were purchased from Qiagen Sciences (Germantown, MD). The cDNA kit and SYBR green qPCR reagent were purchased from Life technologies (Carlsbad, CA). The PAF-R, SOX2 and GAPDH primers were obtained from SABiosciences (Valencia, CA). Caspase-3/7 activity assay kit was purchased from Promega Corporation (Madison, WI). Prostaglandin E2 (PGE2) and F2α (PGF2α) were purchased from Cayman Chemicals Co. (Ann Arbor, MI). Antibodies against cleaved caspase-3, SOX2, β-actin were purchased from Cell Signaling Technology (Danvers, MA).
7
Caspase 3/7 Activity Assay
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Caspase 3/7 activity assay kit (Promega, Madison, WI, USA) was used according to the manufacturer's protocol.
8
Caspase 3/7 Activation Assay
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2,500 cells were transfected with the specified oligos at 25 nM and plated into 96-wells plates. Five days later, caspase 3/7 activity was measured using caspase 3/7 activity assay kit (Promega Inc).
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