Log In Sign up
The largest database of trusted experimental protocols

Aztreonam

Manufactured by AK Scientific
Visit Supplier
Sourced in United States

Aztreonam is a synthetic, monocyclic beta-lactam antibiotic. It is effective against gram-negative aerobic bacteria and has a core function as an antibacterial agent.

Automatically generated - may contain errors

Lab products found in correlation

Cefotaxime, by Merck Group (2 mentions) Sulfamethoxazole, by Merck Group (2 mentions) Erythromycin, by Merck Group (2 mentions) Ciprofloxacin, by Merck Group (2 mentions) Clindamycin, by Merck Group (2 mentions) Cefoxitin, by Merck Group (2 mentions) Tetracycline, by Merck Group (2 mentions) Trimethoprim, by Merck Group (2 mentions) Azithromycin, by AK Scientific (2 mentions) Solid phase extraction cartridges 6cc, by Waters Corporation (2 mentions) 4000 qtrap lc ms ms instrument, by AB Sciex (2 mentions) Penicillin g, by Merck Group (2 mentions) Amoxicillin, by Merck Group (2 mentions) Chloramphenicol, by Merck Group (2 mentions) Doxycycline, by AK Scientific (2 mentions) Cefepime, by AK Scientific (2 mentions) Tigecycline, by AK Scientific (2 mentions) Imipenem, by AK Scientific (1 mentions)

3 protocols using aztreonam

1

Antibiotic Detection in Wastewater: A Comprehensive Approach

Check if the same lab product or an alternative is used in the 5 most similar protocols
Solid phase extraction and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) were utilized to detect the presence of 16 antibiotics from 7 classes in 11 influent and 11 effluent samples supernatants using a modified protocol 33 (link). Fifty ml of influent or effluent sample were centrifuged at 10,000 rpm for 10 minutes; supernatants were concentrated using solid phase extraction cartridges (6cc, 200 mg, Waters, Milford, MA). Samples were analyzed on a 4000 QTRAP LC/MS/MS instrument (AB Sciex). The data was normalized based on the internal standard (isotopically labeled caffeine, 13C3, Cambridge Isotope Laboratories) to account for experimental variation and antibiotic extraction/ionization efficiency. A mixture of the antibiotic standards was also processed along with the samples as positive controls to test for recovery. Standards for amoxicillin, cefotaxime, cefoxitin, chloramphenicol, ciprofloxacin, clindamycin, erythromycin, penicillin G, sulfamethoxazole, tetracycline, and trimethoprim were purchased from Sigma-Aldrich (St. Louis, MO). Standards for azithromycin, aztreonam, cefepime, doxycycline, and tigecycline were purchased from AK Scientific (Union City, CA). All analyses were performed at the Proteomics and Mass Spectrometry Facility at the Donald Danforth Plant Science Center (St. Louis, MO).
Pehrsson E.C., Tsukayama P., Patel S., Mejía-Bautista M., Sosa-Soto G., Navarrete K.M., Calderon M., Cabrera L., Hoyos-Arango W., Bertoli M.T., Berg D.E., Gilman R.H, & Dantas G. (2016). Interconnected microbiomes and resistomes in low-income human habitats. Nature, 533, 212-216.
+ Open protocol
+ Expand
2

Time-Kill Assay of Pan-Resistant Klebsiella

Check if the same lab product or an alternative is used in the 5 most similar protocols
For all experiments, fresh Muller–Hinton Broth (MHB) (Difco, Detroit, MI, USA) was used. Total bacterial counts were determined by plating samples on Muller-Hinton agar (MHA) (Difco, Detroit, MI, USA) followed by incubation at 37 °C for 24 h. This study utilized an unusual pan resistant Klebsiella pneumoniae, that was isolated from a hip abscess of a U.S. patient (BioProject PRJNA391323, GenBank Accession number: CP022125.1-CP022128.1)12 . Previous characterization of this isolate has shown that it harbors four known beta lactamase genes, including plasmid-mediated blaNDM-1 and blaCMY-6, as well as chromosomal blaCTX-M-15 and blaSHV-2. This strain was found to be non-susceptible to a range of drugs tested, including all beta-lactams, colistin, and tigecycline. For time- kill assays, fresh drug stocks of aztreonam and imipenem (AKScientific, Union City, CA, USA) were used at clinically relevant concentrations.
Kaur J.N., Singh N., Smith N.M., Klem J.F., Cha R., Lang Y., Chen L., Kreiswirth B., Holden P.N., Bulitta J.B, & Tsuji B.T. (2024). Next generation antibiotic combinations to combat pan-drug resistant Klebsiella pneumoniae. Scientific Reports, 14, 3148.
+ Open protocol
+ Expand
3

Antibiotic Detection in Wastewater: A Comprehensive Approach

Check if the same lab product or an alternative is used in the 5 most similar protocols
Solid phase extraction and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) were utilized to detect the presence of 16 antibiotics from 7 classes in 11 influent and 11 effluent samples supernatants using a modified protocol 33 (link). Fifty ml of influent or effluent sample were centrifuged at 10,000 rpm for 10 minutes; supernatants were concentrated using solid phase extraction cartridges (6cc, 200 mg, Waters, Milford, MA). Samples were analyzed on a 4000 QTRAP LC/MS/MS instrument (AB Sciex). The data was normalized based on the internal standard (isotopically labeled caffeine, 13C3, Cambridge Isotope Laboratories) to account for experimental variation and antibiotic extraction/ionization efficiency. A mixture of the antibiotic standards was also processed along with the samples as positive controls to test for recovery. Standards for amoxicillin, cefotaxime, cefoxitin, chloramphenicol, ciprofloxacin, clindamycin, erythromycin, penicillin G, sulfamethoxazole, tetracycline, and trimethoprim were purchased from Sigma-Aldrich (St. Louis, MO). Standards for azithromycin, aztreonam, cefepime, doxycycline, and tigecycline were purchased from AK Scientific (Union City, CA). All analyses were performed at the Proteomics and Mass Spectrometry Facility at the Donald Danforth Plant Science Center (St. Louis, MO).
Pehrsson E.C., Tsukayama P., Patel S., Mejía-Bautista M., Sosa-Soto G., Navarrete K.M., Calderon M., Cabrera L., Hoyos-Arango W., Bertoli M.T., Berg D.E., Gilman R.H, & Dantas G. (2016). Interconnected microbiomes and resistomes in low-income human habitats. Nature, 533, 212-216.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!