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S1pr2 h64 s1pr3 h70 erk1 c16 erk 2 c 14 p erk e 4 akt1 2 3 h 136

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S1PR2 (H64), S1PR3 (H70), ERK1 (C16), ERK-2 (C-14), p-ERK (E-4), and Akt1/2/3 (H-136) are laboratory research tools produced by Santa Cruz Biotechnology. These products are antibodies or proteins that can be used to detect and study the corresponding target proteins in biological samples. However, a more detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

S1pr1 ab23695, by Abcam (2 mentions) P akt1 2 3 ser473, by Santa Cruz Biotechnology (2 mentions)

Close spelling variants of this product

P-ERK (271 citations) ERK1/2 (184 citations) P-ERK1/2 (130 citations) Akt1/2/3 (24 citations) P-Akt1/2/3 (9 citations) Akt1/2 (8 citations) P-Akt1 (7 citations) S1PR2 (6 citations) P-ERK/ERK (5 citations) P-ERK (E-4) (3 citations)

2 protocols using s1pr2 h64 s1pr3 h70 erk1 c16 erk 2 c 14 p erk e 4 akt1 2 3 h 136

1

Western Blot Analysis of Protein Signaling

Cited 1 time
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Western blotting analysis of total protein in cell lysates from cultured rat and/or human CCA cell lines was carried out as previously described,17 (link),20 (link) with the following primary Abs purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA): S1PR2 (H64); S1PR3 (H70); ERK1 (C16); ERK-2 (C-14); p-ERK (E-4); Akt1/2/3/ (H-136); and p-Akt1/2/3/ (ser473). S1PR1 (Ab23695) was purchased from Abcam (Cambridge, MA). Protein bands were normalized against β-actin and detected with mouse monoclonal anti-actin Ab (ACTN05) from Thermo Scientific (Wilmington, DE).
Liu R., Zhao R., Zhou X., Liang X., Campbell D.J., Zhang X., Zhang L., Shi R., Wang G., Pandak W.M., Sirica A.E., Hylemon P.B, & Zhou H. (2014). Conjugated bile acids promote cholangiocarcinoma cell invasive growth through activation of sphingosine 1-phosphate receptor 2. Hepatology (Baltimore, Md.), 60(3), 908-918.
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2

Western Blot Analysis of CCA Cell Proteins

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot analysis of total protein in cell lysates from cultured rat and/or human CCA cell lines was carried out as previously described (17 (link), 20 (link)), with the following primary antibodies purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA): S1PR2 (H64), S1PR3 (H70), ERK1 (C16), ERK-2 (C-14), p-ERK (E-4), Akt1/2/3/ (H-136) and p-Akt1/2/3/ (ser473). S1PR1 (Ab23695) was purchased from Abcam (Cambridge, MA). Protein bands were normalized against β-actin, detected with mouse monoclonal anti-actin antibody (ACTN05) from Thermo Scientific (Wilmington, DE).
Liu R., Zhao R., Zhou X., Liang X., Campbell D.J., Zhang X., Zhang L., Shi R., Wang G., Pandak W.M., Sirica A.E., Hylemon P.B, & Zhou H. (2014). Conjugated bile acids promote cholangiocarcinoma cell invasive growth through activation of sphingosine 1-phosphate receptor 2. Hepatology (Baltimore, Md.), 60(3), 908-918.
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