Xt microscope control software

Keratose-paclitaxel coated balloon segments and commercially available DCBs (Lutonix-Bard Peripheral Vascular, Tempe, AZ, United States and SeQuent Please, B. Braun Melsungen AG, Berlin, Germany) were cut from the catheter, mounted on studs, and sputter coated with gold palladium. A Quanta 250 scanning electron microscope with xT Microscope Control software (FEI, Hillsboro, OR, United States) was used for imaging.

A Pro-4 four point system (Lucas Labs, Gilroy, CA, USA) was used in resistivity measurements. The system was made up of a Keithley 2400, a Pro4, and a notebook with the Pro4 V1.2.4 software installed. The system uses the dual configuration test method of ASTM standard F84-99 to compensate for errors in probe spacing and errors caused by proximity to the edge of the conducting layer. A V/I measurement was taken and recorded and the subsequent resistivity was computed.
Scanning electron microscope (SEM) micrographs were obtained using a Quanta 3D FEG Dual Beam FIB/SEM and its xT Microscope Control Software (FEI, Hillsboro, OR, USA). Fluorescence studies were performed on samples using a DM RXA2 fluorescence microscope (Leica, Wetzlar, Germany) fitted with a 100× objective lens with a 1.4 N.A. Slidebook version 4.1 was used for image acquisition from a SensiCam QE camera (Cooke, Auburn Hills, MI, USA) attached to the microscope.
Optical images were obtained from a 40 CFL inverted microscope (Axiovert, Thornwood, NY, USA) fitted with a 20× objective with a 1.0 N.A. AxioVision 4.6.1.0 was used for image acquisition from an AxioCam MRc camera attached to the microscope. Percentage area covered by the NMBs on the glass slides were determined using the ImageJ 1.46r software (Wayne Rasband, National Institutes of Health, USA).