A549, PC-9, NCI-H446 cells were transfected with Lenti-shMTA1, Lenti-MTA1, and Lenti-GFP (The three vectors were purchased from Shanghai GenePharma Co, Ltd. (Shanghai, China) at 40 MOI in 24-well culture plate. Puromycin was used to screen the cells positively infected by the vectors. Finally, western blot analysis was performed to examine the MTA1 expression level. The MTA1-over-expressing cells were separately named as A549-MTA1, PC-9-MTA1, and NCI-H446-MTA1 cells, and MTA1-silencing cells were respectively named as A549-shMTA1, PC-9-shMTA1, and NCI-H446-shMTA1 cells.
Lenti mta1
Manufactured by GenePharma
Sourced in China
Lenti-MTA1 is a laboratory equipment product designed for gene delivery and expression studies. It is a lentiviral vector system that allows for the stable integration and expression of the MTA1 gene in target cells.
Automatically generated - may contain errors
2 protocols using lenti mta1
1
Modulation of MTA1 Expression in Lung Cancer Cells
2
Modulating MTA1 in NSCLC Cell Lines
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Human H460 and H1299 NSCLC cell lines underwent transfection with lentiviral transfer plasmids (lenti) and short-interfering RNA (si-RNA) and were randomly assigned into a control group, a lenti-MTA1 group (MTA1 group), a lenti-si-MTA1 group (si-RNA group), a lenti-control (NC) group, and a si-RNA control group. The lenti-MTA1, lenti-si-MTA1, and lenti-control vectors were purchased from Shanghai GenePharma Co, Ltd. (Shanghai, China). The sequence of the MTA1 si-RNA was: 5′-GACCACCGACAGATACGTG-3′.
Transfection was mediated by lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) (Cat no. 11668-019). A scrambled si-RNA (5′-GACGACGATAAGGGATCCTGA-3′) without homology with the mammalian mRNA sequences, was cloned into the lentivirus vector (GeneChem Co., Ltd. Shanghai, China) as the control si-RNA. Cells were all transfected with 3 μg of plasmid, or empty lentivirus vector, using lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol.
Transfection was mediated by lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) (Cat no. 11668-019). A scrambled si-RNA (5′-GACGACGATAAGGGATCCTGA-3′) without homology with the mammalian mRNA sequences, was cloned into the lentivirus vector (GeneChem Co., Ltd. Shanghai, China) as the control si-RNA. Cells were all transfected with 3 μg of plasmid, or empty lentivirus vector, using lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol.
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