Agilent directdrive scanner

Manufactured by Agilent Technologies

Sourced in United States

The Agilent DirectDrive scanner is a high-performance imaging system designed for life science research applications. It features a direct-drive scanning mechanism that enables fast and precise image acquisition. The scanner produces high-resolution images and supports a range of sample types.

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Lab products found in correlation

Magnevist, by Bayer (1 mentions) Matlab, by MathWorks (1 mentions)

Close spelling variants of this product

Agilent scanner (115 citations) Agilent DirectDrive (4 citations)

5 protocols using agilent directdrive scanner

Diffusion Tensor Imaging of Nerve Repair

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Magnetic resonance imaging was performed on a 4.7-T Agilent Direct Drive scanner (Agilent Technologies, Santa Clara, Calif) for reverse autografts and a 9.4-T Agilent Direct Drive scanner for conduit-repaired nerves. Diffusion tensor imaging data were acquired using a 3-dimensional diffusion-weighted spin-echo sequence with repetition time/echo time of 170/23.0 ms, 12 signal averages, and field of view of 9.6 × 9.6 × 14.4 mm³ for reverse autografts and repetition time/echo time of 160/23.0 ms, 10 signal averages, and field of view of 9.6 × 9.6 × 18.0 mm³ for conduit-repaired nerves. The nominal resolution for both groups was 100 × 100 × 450 μm³ (450 μm along the nerve). Diffusion weighting was achieved with δ/Δ = 4/12 ms, a prescribed b value of 2000 s/mm², and 6 directions. One b = 0 image was acquired for a total of 7 images in a scan time of approximately 12 hours.

Nguyen L., Afshari A., Kelm N.D., Pollins A.C., Shack R.B., Does M.D, & Thayer W.P. (2017). Engineered Porcine-derived Urinary Bladder Matrix Conduits as a Novel Scaffold for Peripheral Nerve Regeneration. Annals of plastic surgery, 78(6), S328-S334.

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MRI Diffusion Tensor Imaging of Nerve Repair

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A subset of animals had nerves imaged with magnetic resonance imaging (MRI) post-operatively (n=3 in each group). Nerves were harvested immediately after repair for imaging. After 24 h of post-fixation, excised nerves were placed in PBS + 2 mM gadopentetic acid (Gd-DTPA) (Magnevist, Bayer HealthCare, Wayne, NJ, USA) at 4 °C for at least 24 h before imaging and trimmed to ~1 cm in length with the injury site centered. MRI was performed on a 9.4T 21-cm horizontal bore Agilent DirectDrive scanner (Agilent Technologies, Santa Clara, CA, USA) using a 38-mm Litz quadrature coil (Doty Scientific, Columbia, SC, USA) for radio frequency (RF) transmission and reception. Diffusion tensor imaging (DTI) data were acquired using a three-dimensional diffusion-weighted spin-echo sequence. Image data reconstruction was performed using in-house written code in MATLAB (Mathworks, Natick, MA, USA). Diffusion tensor estimation and tractography were performed using ExploreDTI (exploredti.com).

Bamba R., Riley D.C., Kelm N.D., Cardwell N., Pollins A.C., Afshari A., Nguyen L., Dortch R.D, & Thayer W.P. (2017). A Novel Conduit-Based Coaptation Device for Primary Nerve Repair. The International journal of neuroscience, 128(6), 563-569.

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High-Resolution Diffusion Tensor Imaging of Peripheral Nerves

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The MRI was performed on a 4.7-T 31-cm horizontal bore Agilent DirectDrive scanner (Agilent Technologies, Santa Clara, Calif) using a 38-mm Litz quadrature coil (Doty Scientific, Columbia, SC) for radiofrequency transmission and reception. For excised nerve imaging, field of view = 9.6 × 9.6 × 12 mm3 and matrix size = 96 × 96 × 32 for a nominal resolution of 100 × 100 × 375 mm3 (375 mm along the nerve). For hind limb imaging, field of view = 48.0 × 25.6 × 28.8 mm3 and matrix size = 192 × 128 × 144 for a nominal resolution of 250 × 200 × 200 mm3 (250 mm along the nerve). Diffusion tensor imaging (DTI) data were acquired using a 3D diffusion-weighted spin-echo sequence with TR/TE of 170/23.0 milliseconds and 12 signal averages for excised nerves, and TR/TE of 170/22.1 milliseconds and 2 signal averages for hind limbs. Diffusion weighting was achieved with δ/Δ = 4/12 milliseconds, prescribed b-value = 2000 seconds/mm2, and 6 directions. One b = 0 image was acquired for a total of 7 images in a scan time of 12 hours.

Riley D.C., Boyer R.B., Deister C.A., Pollins A.C., Cardwell N.L., Kelm N.D., Does M.D., Dortch R.D., Bamba R., Shack R.B, & Thayer W.P. (2017). Immediate Enhancement of Nerve Function Using a Novel Axonal Fusion Device After Neurotmesis. Annals of Plastic Surgery, 79(6), 590-599.

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High-Resolution Diffusion Tensor Imaging of Peripheral Nerves

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Magnetic resonance imaging was performed on a 4.7-T 31-cm horizontal bore Agilent DirectDrive scanner (Agilent Technologies, Santa Clara, Calif) using a 38-mm Litz quadrature coil (Doty Scientific, Columbia, SC) for radiofrequency transmission and reception. Diffusion tensor imaging data were acquired using a 3-dimensional (3D) diffusion-weighted spin echo sequence with repetition time/echo time = 170/ 23.0 ms, 12 signal averages, field-of-view = 9.6 × 9.6 × 14.4 mm³, and matrix size = 96 × 96 × 32 for a nominal resolution of 100 × 100 × 450 μm³ (450 μm along the nerve). Diffusion weighting was achieved with δ/Δ = 4/12 ms, a prescribed b value of 2000 s/mm², and 6 directions. One b = 0 image was acquired for a total of 7 images in a scan time of approximately 12 hours.

Afshari A., Nguyen L., Kelm N.D., Kim J.S., Cardwell N.L., Pollins A.C., Bamba R., Shack R.B., Does M.D, & Thayer W.P. (2018). Assessment of the Effect of Autograft Orientation on Peripheral Nerve Regeneration Using Diffusion Tensor Imaging. Annals of plastic surgery, 80(4), 384-390.

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High-Resolution Diffusion Tensor Imaging of Peripheral Nerves

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MRI was performed on a 4.7-T, 31-cm horizontal bore, Agilent DirectDrive scanner (Agilent Technologies) using a 38-mm Litz quadrature coil (Doty Scientific) for radio-frequency transmission and reception. For excised nerve imaging, the FOV was 9.6 × 9.6 × 12 mm³ and the matrix size was 96 × 96 × 32 for a nominal resolution of 100 × 100 × 375 μm³ (375 μm along the nerve). For hindlimb imaging, the FOV was 48.0 × 25.6 × 28.8 mm³ and the matrix size was 192 × 128 × 144, for a nominal resolution of 250 × 200 × 200 μm³ (250 μm along the nerve). DTI data were acquired using a 3D diffusion-weighted spin-echo sequence with a TR/TE of 170/23.0 msec and 12 signal averages for excised nerves, and a TR/TE of 170/22.1 msec and 2 signal averages for hind limbs. Diffusion weighting was achieved with a δ/Δ of 4/12 msec, a prescribed b value of 2000 sec/mm², and 6 directions. One b = 0 image was acquired, for a total of 7 images in a scan time of approximately 12 hours.

Boyer R.B., Kelm N.D., Riley D.C., Sexton K.W., Pollins A.C., Shack R.B., Dortch R.D., Nanney L.B., Does M.D, & Thayer W.P. (2015). 4.7-T diffusion tensor imaging of acute traumatic peripheral nerve injury. Neurosurgical focus, 39(3), E9.

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