Pathogen‐free 6–8‐week‐old BALB/c, BALB/c SCID (Harlan Sprague‐Dawley, Indianapolis, IN) and IFN‐γ KO mice with the respective BALB/c background mice were purchased from Jackson Laboratories (Bar Harbor, ME). The mice were housed and maintained under pathogen‐free conditions. All animal procedures were approved by the University of Cincinnati Institutional Animal Care and Use Committee. The mice were housed in a barrier facility, maintained in sterile conditions in microisolator cages, and supplied with sterilized food and water. All experimental animals were maintained in microisolator cages until use.
Ifn γ ko mice
Manufactured by Jackson ImmunoResearch
IFN‐γ KO mice are genetically modified mice that lack the gene encoding for the cytokine interferon-gamma (IFN-γ). These mice are used as a model to study the role of IFN-γ in various biological processes and disease states.
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2 protocols using ifn γ ko mice
1
BALB/c Mouse Maintenance Protocol
2
Transgenic Mouse Model for GRIM-19 Study
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Six-to-eight-week-old male mice (strains C57BL/6 and IFNγ-KO) mice were purchased from Jackson Laboratory. Mouse GRIM-19 transgenic mice (C57BL/6 background) were purchased from Macrogen (Seoul, Korea). To establish this mouse line, a GRIM-19 fragment was inserted into the pcDNA3.1+ vector (Invitrogen, Waltham, MA, USA) containing the cytomegalovirus promoter by GenScript Corporation (Piscataway, NJ, USA) and microinjected by Macrogen. All animals were maintained under specific pathogen-free (SPF) conditions with free access to standard mouse chow (Ralston Purina, St. Louis, MO, USA) and water. The Animal Care Committee of The Catholic University of Korea approved the experimental protocol. All experimental procedures were evaluated and carried out in accordance with the protocols approved by the Animal Research Ethics Committee at the Catholic University of Korea (ID number: CUMC-2017-0304-02). All procedures performed followed the ethical guidelines on animal use.
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