The content of indicators of protein metabolism (total protein, albumin, globulins, urea, creatinine), lipid metabolism (total cholesterol, HDL cholesterol, LDL cholesterol, triglycerides), carbohydrate metabolism (glucose), purine metabolism (uric acid), mineral metabolism (calcium, phosphorus, magnesium), and functional state of the liver (total bilirubin, ALT, AST, alkaline phosphatase) in the blood serum of animals was determined on an automatic biochemical analyzer “Konelab 20i” (ThermoScientific, Waltham, MA, USA). Fat was extracted from the liver using the Folch method [60 (link)]. The content of triglycerides and cholesterol in fat extracted from the liver was determined photometrically on an automatic biochemical analyzer Konelab 20i (ThermoScientific, Waltham, MA, USA).
Konelab 20i
Manufactured by Thermo Fisher Scientific
Sourced in Finland, France, United States, Germany
The Konelab 20i is a clinical chemistry analyzer designed for in-vitro diagnostic testing. It automates the analysis of various clinical chemistry parameters using a photometric detection method. The Konelab 20i is capable of performing a range of routine clinical chemistry tests to support medical diagnosis and patient care.
Automatically generated - may contain errors
Lab products found in correlation
Serum and edta tubes, by Sarstedt (2 mentions)
High performance liquid chromatography (hplc), by Waters Corporation (1 mentions)
Cobas 6000 analyser, by Roche (1 mentions)
Glp 1 active elisa kit, by Merck Group (1 mentions)
Insulin ct, by PerkinElmer (1 mentions)
Procyte dxtm, by IDEXX (1 mentions)
Uv 6300pc, by Avantor (1 mentions)
Bc 3600, by Mindray (1 mentions)
Cobas integra 400 plus, by Roche (1 mentions)
Cell dyn 3500 analyzer, by Abbott (1 mentions)
Cobas e 410, by Roche (1 mentions)
Elecsys 2010, by Roche (1 mentions)
Cobas integra 400, by Roche (1 mentions)
Compact plus device, by Thermo Fisher Scientific (1 mentions)
Brahms copeptin proavp kryptor, by Thermo Fisher Scientific (1 mentions)
Calcium as fs, by DiaSys (1 mentions)
Magnesium xl fs, by DiaSys (1 mentions)
41 protocols using konelab 20i
1
Comprehensive Metabolic Assessment in Animals
2
Oxidative Stress Profile in Pigs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
White blood cell count and plasma DAO concentrations were determined from blood collected on EDTA. The other plasma variables were measured from blood collected on heparin. Total number of WBC and the differential count of granulocytes (eosinophils, basophils, and neutrophils) were measured with a hematology automated cell counter calibrated for pigs (MS9®; Melet Schloesing Laboratories, Osny, France). Automated enzymatic methods using a multianalyzer apparatus (Konelab20i, Thermo Electron Corporation, Cergy, France) were used to determine plasma concentrations of dROM and biological antioxidant potential (BAP) (dROM and BAP tests, references MC003 and MC437, respectively; Diacron, Grosseto, Italy). Oxidative stress index was calculated as the dROM to BAP ratio as previously described [13 (link)]. Plasma concentrations of vitamins E and A were assayed by liquid chromatography (HPLC, Waters Alliance) on a dedicated column (Chromsystems, Germany). Quantitative sandwich ELISA tests (Catalog Number MBS9718395) were used to measure DAO concentrations.
3
Fasting Blood Analysis of Metabolic Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After a 12-hour overnight fasting, venous blood samples were collected and centrifuged in order to obtain serum. The biochemical parameters were analyzed by a Konelab 20i chemistry analyzer (Thermo Electron Corporation, Vantaa, Finland) according to standard procedures. Serum C-reactive protein (CRP) levels were determined by immunonephelometry (GOLDSITE Diagnostics, Inc., Shenzhen, China). Serum insulin levels were detected with an enzyme-linked immunosorbent assay (ELISA) kit (NovaTec Immundiagnostica GmbH, Dietzenbach, Germany) following the manufacturer's instructions. The lowest detectable concentration of insulin was 0.25 μIU/mL at a 95% confidence limit; the intra-assay variability was within ≤5%. Homeostasis model assessment for estimating insulin resistance (HOMA-IR) which was calculated as the product of fasting glucose concentration (mg/dL) and fasting insulin concentration was divided by 405. Erythrocyte sedimentation rate (ESR) was measured by the Wintrobe method.
4
Fasting Blood Sampling and Insulin Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Venous blood samples were collected after 8–10 h of overnight fasting, both at baseline (T0) and T1. The serum was obtained after centrifugation at 3000 rpm for 10 min and stored in sterile tubes at 4 °C for no more than 4 h during the morning of collection. The biochemical parameters were determined by a Konelab 20i chemistry analyzer (Thermo Electron Corporation, Vantaa, Finland) according to standard procedures. Subsequently, the serum samples were stored at −80 °C. Serum insulin levels were measured with an enzyme-linked immunosorbent assay (Novatec Immundiagnostica GmbH, Dietzenbach, Germany) following the manufacturer’s instructions. The lowest detectable insulin concentration was 0.25 μIU/mL at a 95% confidence limit; the intra-assay variability was within ≤5%.
5
Biochemical Panel: Plasma and Urine Analyses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma concentrations of total proteins, urea, creatinine, alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), and urine concentrations of uric acid, urea, creatinine, proteins, were measured by an automated spectrophotometric system Konelab 20i (from thermo electron corporation, Cergy-Pontoise, France) using the manufacturer^ biological chemistry reagents (Manens et al. 2016) .
6
Measurement of Inflammatory Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Interleukin 1β, interleukin 6 and YKL-40 were determined with commercially available ELISA tests, according to the recommendations of the manufacturer. Human IL-1β ELISAPRO kit (MABTECH AB, Nacka Strand, Stockholm, Sweden) was used for Interleukin 1β concentrations, High Sensitivity ELISA kit (The Covalab, Villeurbanne, France) for interleukin 6 and Human Chitinase-3-like Protein 1 ELISA Kit (Bioassay Technology Laboratory, Shanghai, China) for YKL-40 concentrations. In order to determine concentrations of the aforementioned inflammatory parameters, Mindray-96A reader was used. C-reactive protein and IgG concentrations were measured using the immunoturbidimetric method (highly sensitive for CRP, U-hs, DiaSys Diagnostic Systems GmbH, Germany and immunoglobulin G FS, DiaSys Diagnostic Systems GmbH, Holzheim, Germany, respectively), using the biochemical analyser Konelab 20i® (ThermoScientific, Vantaa, Finland). CA 125 and prolactin concentrations were measured by Cobas® 6000 analyser (Roche, Mannheim, Germany).
7
Measuring Glucose and Insulin Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Non fasting insulin and glucose plasma levels were determined in db/db and db/+ control mice by ELISA kit (#EZRMI-13K, Merck Millipore, Fontenay sous bois, France) according to the manufacturer’s instructions and glucose GOD-POD kit (#981780, Thermo Fischer Scientific, Illkirch, France) optimized for Konelab analyzer (Konelab 20i, Thermo Fisher Scientific).
8
Plasma Metabolite Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A jugular blood sample (10 mL) collected on EDTA was taken at exsanguination. Plasma was immediately prepared by low speed centrifugation (15 min at 2500g) at 4°C, and stored at -20°C until analyses. Plasma concentrations of glucose, urea, triglycerides (TG) and non-esterified fatty acid (NEFA) were determined in duplicate using commercial kits and a clinical chemistry analyzer (Konelab 20i, Thermo Fisher Scientific, Courtaboeuf, France). Intra-assay coefficients of variation for measurements were below 5%.
9
Biochemical Analysis of Heparinized Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For biochemical analysis, a part of heparinized blood, after centrifugation at 855 g for 10 min at cooled centrifuge (4°C), was used. Biochemical parameters including albumins (ALB), total proteins (TP), glucose (GLU), ammonia (NH3), triacylglycerols (TRIG), lactate (LACT), cholesterol (CHOL), alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), calcium (Ca2+), and inorganic phosphate (PHOS). Plasma biochemical indicators were measured using a biochemical automatic analyzer Konelab 20i (ThermoScientific, Czech Republic) and commercial test kits (BioVendor, Czech Republic).
10
Biochemical Analyte Measurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Concentrations of albumin, total bilirubin, uric acid, and iron were measured by colorimetric method using the biochemical analyzer Konelab 20i® (ThermoScientific, Vantaa, Finland). All procedures were performed following the manufacturers' instructions. Lower test limits were 2.00 g/dL, 0.06 mg/dL, 0.20 mg/dL, and 6.00 μg/dL, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!