Log In Sign up
The largest database of trusted experimental protocols

Eclipse c1 confocal microscope

Manufactured by Nikon
Visit Supplier
Sourced in Italy, Japan

The Eclipse C1 confocal microscope is a high-resolution imaging system designed for advanced research applications. It provides precise optical sectioning and enhanced contrast for detailed visualization of biological samples. The Eclipse C1 utilizes a laser-scanning technology to capture clear, high-quality images, enabling researchers to explore their specimens in depth.

Automatically generated - may contain errors

Lab products found in correlation

Eclipse te2000 e, by Nikon (3 mentions) 3 3 diaminobenzidine substrate, by Vector Laboratories (2 mentions) Ez c1, by Nikon (2 mentions) βiii tubulin, by Merck Group (1 mentions) Glut3, by Santa Cruz Biotechnology (1 mentions) Biotin conjugated anti rabbit igg, by Vector Laboratories (1 mentions) Nestin, by Thermo Fisher Scientific (1 mentions) Cd133, by Miltenyi Biotec (1 mentions) Glial fibrillary acidic protein (gfap), by Cell Signaling Technology (1 mentions) 25 mm round glass coverslips, by Warner Instruments (1 mentions) Alexa fluor, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 labeled donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Neurolucida explorer, by MBF Biosciences (1 mentions) Dq green bsa, by Thermo Fisher Scientific (1 mentions) Hard set, by Vector Laboratories (1 mentions) Eclipse c1, by Nikon (1 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions) Anti β tubulin, by Fortrea (1 mentions) Te2000 u inverted microscope, by Nikon (1 mentions)

19 protocols using eclipse c1 confocal microscope

1

Immunohistochemical Analysis of Neural Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunohistochemical staining of formalin-fixed paraffin-embedded tissues, antigen retrieval was performed in citrate buffer at pH 6.0 and 95°C for 20 minutes. Sections were blocked then incubated overnight at 4°C in primary antibody integrin αvβ3 (LM609) or β3 (Cell signaling), Glut3 (Santa Cruz Biotechnology), GFAP (Cell Signaling), βIII tubulin (Sigma-Aldrich), Nestin (Fisher Scientific), CD133 (Miltenyi Biotech) followed by biotin-conjugated anti-rabbit IgG and an avidin-biotin peroxidase detection system with 3,3′-diaminobenzidine substrate (Vector Labs) and counterstained with hematoxylin. Double-immunostaining for β3/Glut3 was carried out according to manufacturer recommendations (Vector Labs). A Nikon Eclipse C1 Confocal microscope as well as a Nikon Eclipse TE2000-E were used for imaging.
Cosset É., Ilmjärv S., Dutoit V., Elliott K., von Schalscha T., Camargo M.F., Reiss A., Moroishi T., Seguin L., Gomez G., Moo J.S., Preynat-Seauve O., Krause K.H., Chneiweiss H., Sarkaria J.N., Guan K.L., Dietrich P.Y., Weis S.M., Mischel P.S, & Cheresh D.A. (2017). Glut3 addiction is a druggable vulnerability for a molecularly defined subpopulation of glioblastoma. Cancer cell, 32(6), 856-868.e5.
+ Open protocol
+ Expand
2

Confocal Microscopy of Transfected SH-SY5Y Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
SH-SY5Y cells were seeded at 4.0x105 cells in 35 mm dishes containing 25 mm round glass coverslips (Warner Instruments) and allowed to adhere overnight. Cells were transfected with the GCaMPer plasmid DNA using 2 μL Lipofectamine 2000 (Invitrogen) and 0.4 μg DNA per well. Forty hours after transfection, cells were fixed with 4% paraformaldehyde in PBS (pH 7.4), permeabilized with PBS + 0.1% TritonX-100 + 0.2% BSA for 15 min. Permeabilized cells were blocked with PBS + 0.1% TritonX-100 + 5% goat serum for 1 h at room temperature. Primary antibodies (described in antibody reagents section) were prepared in fresh blocking solution, added to cells, and incubated overnight at 4°C. Secondary antibodies (AlexaFluor, Invitrogen) were added for 1 h at room temperature. All washes were done with PBS + 0.1% TritonX-100 at room temperature and nuclei were stained with 1 μg/mL DAPI (in PBS) for 15 min. Coverslips were mounted on glass slides with Mowiol 4–88 (EMD) and allowed to set overnight. A Nikon Eclipse-C1 confocal microscope equipped with a Nikon 100X (1.30 NA) PlanFluor objective was used to image samples. Nikon EZ-C1 software was used for image acquisition. Micrographs were prepared using Adobe Photoshop or ImageJ (NIH).
Henderson M.J., Baldwin H.A., Werley C.A., Boccardo S., Whitaker L.R., Yan X., Holt G.T., Schreiter E.R., Looger L.L., Cohen A.E., Kim D.S, & Harvey B.K. (2015). A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic Reticulum Calcium Store. PLoS ONE, 10(10), e0139273.
+ Open protocol
+ Expand
3

Immunostaining of RyR and DHPR in Myotubes

Check if the same lab product or an alternative is used in the 5 most similar protocols
RyR-null (dyspedic) myotubes expressing either WT RyR or RyR K-Q mutant that were plated on glass coverslips were fixed and immunostained with a mouse monoclonal anti-RyR antibody (34C, 1:10; Developmental Studies Hybridoma Bank) and a sheep polyclonal anti-DHPR antibody (1:200; Upstate Biotechnology) overnight at 4 °C as previously described [41 (link)]. On the following day, coverslips were washed with PBS three times each for 5 min and then incubated for 1 h at room temperature in blocking buffer containing a 1:500 dilution of Alexa Fluor 488–labeled donkey anti-mouse IgG (Molecular Probes) and 1:500 dilution of rhodamine-labeled donkey anti-sheep IgG (Jackson ImmunoResearch Laboratories Inc.) and washed with PBS (three times for 5 min each). Coverslips were mounted on glass slides and images obtained using a Nikon Eclipse-C1 confocal microscope (Nikon Instruments Inc.) and a 40× oil objective. All confocal images were sampled at a spatial resolution (pixel diameter) of 100 nm.
Rebbeck R.T., Willemse H., Groom L., Casarotto M.G., Board P.G., Beard N.A., Dirksen R.T, & Dulhunty A.F. (2015). Regions of ryanodine receptors that influence activation by the dihydropyridine receptor β1a subunit. Skeletal Muscle, 5, 23.
+ Open protocol
+ Expand
4

Quantitative Confocal Imaging Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Confocal images were acquired with a Nikon Eclipse-C1 confocal microscope (Nikon Instruments, Firenze, Italy) under a 40x objective with 1 N/A at 100 Hz scan speed, format 1024x1024 pixels, resolution 0.31 μm/pixel, Zstep size 1μm and a color depth of 8 bits per channel, through the EZ-C1 software (Nikon Instruments, Firenze, Italy). Laser intensity, gain and offset were maintained constant in each analysis and the intensity of the signal never reached saturation levels.
Quantitative and morphometric evaluations on samples were made by investigators blinded to genotype or treatment using the software ImageJ (http://rsbweb.nih.gov/ij/index.html) or Neurolucida (Bioscience, Williston, USA, version 10.30.1) which was connected to an E-800 Nikon microscope via a color CCD camera to trace dendrites live, under a 20x objective with 0.50 N/A, 0.36 μm/pixel resolution, to subsequently import data to Neurolucida Explorer (MBF Bioscience, Williston, USA) for the Sholl analysis. Exposure time, gain and offset were, also in this case, kept constant during the whole study. Adobe Photoshop (Adobe Systems, San Jose, CA) was used to assemble the final figures and to adjust image contrast on the entire figure, after all the analyses were completed.
Bertocchi I., Mele P., Ferrero G., Oberto A., Carulli D, & Eva C. (2021). NPY-Y1 receptor signaling controls spatial learning and perineuronal net expression. Neuropharmacology, 184.
+ Open protocol
+ Expand
5

3D Macropinosome Imaging and Tumor Labeling

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
As described [1 (link), 46 (link)], cells were grown in 3D using poly-HEMA coated plates in serum-free media for 3 hours. Macropinosomes were marked using a high-molecular TMR-dextran and/or DQ-Green BSA (Life Technologies) at a final concentration of 1 mg/ml for 1 hour at 37°C. Cells were rinsed in cold PBS, fixed in 3.7% formaldehyde, and coverslips mounted using Hard Set (Vector Labs). Images were captured using a Nikon Eclipse C1 confocal microscope with 1.4 NA 60× oil-immersion lens and minimum pinhole (30 μm), and analyzed using the ‘Analyze Particles’ feature in ImageJ. Particle area per cell was determined from at least three randomly selected fields, each containing approximately 75–150 cells depending on cell size and seeding density. As described for the ex vivo assay [47 (link)], tumors were cut into 3-mm cubes, immersed in serum-free RPMI with 1 mg/ml of FITC-dextran at 37°C for 1 hour, rinsed in PBS, and frozen in optimal cutting temperature (OCT) compound.
Seguin L., Camargo M.F., Wettersten H.I., Kato S., Desgrosellier J.S., von Schalscha T., Elliott K.C., Cosset E., Lesperance J., Weis S.M, & Cheresh D.A. (2017). Galectin-3, a druggable vulnerability for KRAS-addicted cancers. Cancer discovery, 7(12), 1464-1479.
+ Open protocol
+ Expand
6

3D Cell Culture Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The 3D culture assay was performed using a Cellrix 3D Culture System Kit (B1000-096; MediFab, Seoul, Korea) according to the manufacturer’s instructions. Briefly, cultured cells were trypsinized and stained with DiI (2 g/ml) for 10 min, and then resuspended at 1 × 106 cells/ml in Cellrix Bio-Gel. The casting mold was removed from the gel and cells contained in Cellrix Bio-Gel were loaded onto the casting gel. After incubation on ice for 15 min, the gel was transferred to 96-well plates, and medium containing premixed vehicle [0.001% DMSO] and/or inhibitor was added, with medium change every 3 days. DiI fluorescence images were obtained with an Eclipse C1 confocal microscope (Eclipse C1; Nikon, Tokyo, Japan) and analyzed with AxioVision v.4.3 software (Carl Zeiss, Oberkochen, Germany).
Kim H.K., Bhattarai K.R., Junjappa R.P., Ahn J.H., Pagire S.H., Yoo H.J., Han J., Lee D., Kim K.W., Kim H.R, & Chae H.J. (2020). TMBIM6/BI-1 contributes to cancer progression through assembly with mTORC2 and AKT activation. Nature Communications, 11, 4012.
+ Open protocol
+ Expand
7

Live Imaging of Larval Drosophila Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols
Drosophila strain W[1118], stock number 5905 was obtained from Bloomington stock center (Indiana University, USA) and used to perform live imaging experiments. Fly larvae having reached the third instar stage whilst feeding on standard food were dissected in hemolymph-like HL3 saline (70 mM NaCl, 5 mM KCl, 1.5 mM CaCl2, 20 mM MgCl2, 10 mM NaHCO3, 5 mM trehalose, 115 mM sucrose, 5 mM sodium HEPES, pH 7.2). For live tissue imaging ER-Tr red and NRB-AF12 were added to the medium of dissected larvae and images acquired using a Nikon eclipse C1 confocal microscope and a Nikon Fluor 60x NA 1.00 water objective.
D'Amore C., Orso G., Fusi F., Pagano M.A., Miotto G., Forgiarini A., De Martin S., Castellani G., Ribaudo G., Rennison D., Brimble M.A., Hopkins B., Ferrarese A, & Bova S. (2016). An NBD Derivative of the Selective Rat Toxicant Norbormide as a New Probe for Living Cell Imaging. Frontiers in Pharmacology, 7, 315.
+ Open protocol
+ Expand
8

Immunocytochemistry of Glial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
GSCs were plated on coverslips coated with poly-L-ornithine and were grown in DMEM complete medium for 2 weeks. Cells were fixed in 4% PFA and incubated overnight with the following antibodies: GFAP (Sigma-Aldrich) and anti-β-Tubulin (Covance). After washing, anti-mouse Alexa565 and anti-rabbit Alexa 488 were used as secondary antibodies. Nuclei were counterstained with DAPI. Image acquisition was done with a Nikon Eclipse C1 Confocal microscope.
Cosset É., Ilmjärv S., Dutoit V., Elliott K., von Schalscha T., Camargo M.F., Reiss A., Moroishi T., Seguin L., Gomez G., Moo J.S., Preynat-Seauve O., Krause K.H., Chneiweiss H., Sarkaria J.N., Guan K.L., Dietrich P.Y., Weis S.M., Mischel P.S, & Cheresh D.A. (2017). Glut3 addiction is a druggable vulnerability for a molecularly defined subpopulation of glioblastoma. Cancer cell, 32(6), 856-868.e5.
+ Open protocol
+ Expand
9

Quantifying neuronal mRNA-RBP colocalization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Confocal images were acquired by a Nikon Eclipse C1 confocal microscope mounted on a Nikon TE-2000U inverted microscope using a Plan-Apochromat 60x/1.4 oil-immersion objective. Sequential scanning laser was used to avoid cross talk between different fluorochromes. Only pyramidal neurons with clear dendrites were acquired, with a z-stack thickness of 200 nm (12 stacks). At least 21 neurons from three independent experiments were measured for colocalization analysis. Regions of interest (ROI) had a 10 μm × 5 μm × 2.4 μm size located in the proximal (10 μm far from soma) and distal (at least 60 μm far from soma) compartments of apical dendrites. After ROI selection images were cropped, then background was automatically subtracted using Imaris software (Bitplane). Images were then deconvolved using Huygens software with classical CMLE algorithm. “Coloc” function of Imaris was used to perform colocalization analysis Automatic threshold was applied to each image before Manders coefficients calculation. Images of colocalization between mRNA and RBPs are Z-stack maximum projection of representative dendrite shafts analyzed. The images were post-processed to linearly increase brightness equally in the whole panel for visualization purpose.
Vicario A., Colliva A., Ratti A., Davidovic L., Baj G., Gricman Ł., Colombrita C., Pallavicini A., Jones K.R., Bardoni B, & Tongiorgi E. (2015). Dendritic targeting of short and long 3′ UTR BDNF mRNA is regulated by BDNF or NT-3 and distinct sets of RNA-binding proteins. Frontiers in Molecular Neuroscience, 8, 62.
+ Open protocol
+ Expand
10

Quantifying Neurodegeneration and Synaptic Alterations

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunofluorescence and in situ hybridization, we acquired images with an Eclipse C1 confocal microscope and software (Nikon). We quantified the number of NeuN neurons from fluorescence images of four tTA:CHMP2BWT mice and four tTA:CHMP2BIntron5 mice at 8 months of age; observers were blinded to genotype. For NeuN counting, we counted three to four sections of the mPFC with the Image J cell-counter plug-in.
For Gria puncta quantification, we used confocal z-stacks (159 × 159 × 10 μm). We analyzed three to four sections (n = 3 mice per genotype) or two sections (n = 6 mice per AAV vector). Following z-projection images, we quantified puncta using Particle Analyzer plug-in. We carried out GFAP coverage quantification as previously described14 (link).
Gascon E., Lynch K., Ruan H., Almeida S., Verheyden J., Seeley W.W., Dickson D.W., Petrucelli L., Sun D., Jiao J., Zhou H., Jakovcevski M., Akbarian S., Yao W.D, & Gao F.B. (2014). Alterations in microRNA-124 and AMPA receptors contribute to social behavioral deficits in frontotemporal dementia. Nature medicine, 20(12), 1444-1451.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!