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Female c57bl 6 mice

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C57BL/6 female mice are a commonly used inbred mouse strain. They are widely utilized in biomedical research due to their well-characterized genetic background and physiology.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Penicillin, by Thermo Fisher Scientific (4 mentions) Female balb c mice, by Jackson ImmunoResearch (3 mentions) C57bl 6 male mice, by Jackson ImmunoResearch (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) D12492, by Research Diets (3 mentions) Ot 1 tcr transgenic mice, by Jackson ImmunoResearch (2 mentions) C57bl 6 tg cag egfp 131osb leysopj, by Jackson ImmunoResearch (2 mentions) Female nod mice, by Jackson ImmunoResearch (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (2 mentions) Fitc anti mouse cd3, by BioLegend (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) C57bl 6 mice, by Jackson ImmunoResearch (2 mentions) Dht pellets, by Innovative Research (2 mentions) Tcrα female mice, by Jackson ImmunoResearch (2 mentions) Lck promoter, by Jackson ImmunoResearch (2 mentions) Easysep neutrophil isolation kit, by STEMCELL (2 mentions) Living image, by PerkinElmer (2 mentions)

Close spelling variants of this product

C57BL/6 females (30 citations) C57BL/6 mice (4025 citations) C57BL/6 (2462 citations)

327 protocols using female c57bl 6 mice

1

Animal Models for SARS-CoV-2 Research

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Female BALB/c mice with 10–15 weeks of age and female C57BL/6 mice with 14-15 weeks of age, as well as Female BALB/c mice lacking CD1d1 and CD1d2 genes (strain: C.129S2-Cd1tm1Gru/J) and female C57BL/6 mice lacking IFN- γ (strain: B6.129S7-Ifngtm1Ts/J) were purchased from The Jackson Laboratory (Bar Harbor, ME) and maintained under specific pathogen-free conditions in the animal facility at Columbia University Irving Medical Center. Heterozygous 8-9 weeks old female K18-hACE C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J) were obtained from The Jackson Laboratory and housed in a pathogen-free animal facility at Washington University School of Medicine. Male Syrian hamsters with 5–6 weeks of aged were purchased from Charles River Laboratories (Wilmington, MA) and housed in an enhance biosafety level 3 (BL3) facility at Washington University in St. Louis.
Tsuji M., Nair M.S., Masuda K., Castagna C., Chong Z., Darling T.L., Seehra K., Hwang Y., Ribeiro Á.L., Ferreira G.M., Corredor L., Coelho-dos-Reis J.G., Tsuji Y., Mori M., Boon A.C., Diamond M.S., Huang Y, & Ho D.D. (2023). An immunostimulatory glycolipid that blocks SARS-CoV-2, RSV, and influenza infections in vivo. Nature Communications, 14, 3959.
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2

Tumor Growth Modulation via SPOP and PD-L1

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1 × 105 B16-F10 or 2 × 105 MC38 cells were injected subcutaneously into 6-weeks old C57BL/6 female mice (from the Jackson Laboratory). Starting one week later, mice were treated daily with palbociclib (150 mg/kg body weight, by gastric gavage) or vehicle only, for 7 days. Subsequently, tumors were collected and analyzed by FACS or immunoblotting.
1 × 105 B16-F10 cells stably expressing SPOP WT or F102C mutant were injected subcutaneously into 6-weeks old C57BL/6 female mice (from the Jackson Laboratory). On day 3 after tumor cells were injected, control and PD-L1 mAb treatments were conducted by intra-peritoneal injection (200 μg/mouse in 200 μl HBSS saline buffer) every three days for a total of 3 injections. Subsequently, tumors were collected and analyzed by FACS.
1 × 105 B16-F10 cells stably expressing SPOP WT or F102C mutant were injected subcutaneously into 6-weeks old Tcrα−/− female mice (from the Jackson Laboratory). After 10 days, tumors were collected and analyzed by FACS.
Zhang J., Bu X., Wang H., Zhu Y., Geng Y., Nihira N.T., Tan Y., Ci Y., Wu F., Dai X., Guo J., Huang Y.H., Fan C., Ren S., Sun Y., Freeman G.J., Sicinski P, & Wei W. (2017). Cyclin D-CDK4 kinase destabilizes PD-L1 via Cul3SPOP to control cancer immune surveillance. Nature, 553(7686), 91-95.
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3

Tumor Growth Modulation via SPOP and PD-L1

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1 × 105 B16-F10 or 2 × 105 MC38 cells were injected subcutaneously into 6-weeks old C57BL/6 female mice (from the Jackson Laboratory). Starting one week later, mice were treated daily with palbociclib (150 mg/kg body weight, by gastric gavage) or vehicle only, for 7 days. Subsequently, tumors were collected and analyzed by FACS or immunoblotting.
1 × 105 B16-F10 cells stably expressing SPOP WT or F102C mutant were injected subcutaneously into 6-weeks old C57BL/6 female mice (from the Jackson Laboratory). On day 3 after tumor cells were injected, control and PD-L1 mAb treatments were conducted by intra-peritoneal injection (200 μg/mouse in 200 μl HBSS saline buffer) every three days for a total of 3 injections. Subsequently, tumors were collected and analyzed by FACS.
1 × 105 B16-F10 cells stably expressing SPOP WT or F102C mutant were injected subcutaneously into 6-weeks old Tcrα−/− female mice (from the Jackson Laboratory). After 10 days, tumors were collected and analyzed by FACS.
Zhang J., Bu X., Wang H., Zhu Y., Geng Y., Nihira N.T., Tan Y., Ci Y., Wu F., Dai X., Guo J., Huang Y.H., Fan C., Ren S., Sun Y., Freeman G.J., Sicinski P, & Wei W. (2017). Cyclin D-CDK4 kinase destabilizes PD-L1 via Cul3SPOP to control cancer immune surveillance. Nature, 553(7686), 91-95.
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4

Anesthesia and Blood Sampling in Mice

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Female C57BL/6 mice (6–8 weeks of age, approximately 20 g) were obtained from Jackson Laboratory. Animal studies were specifically approved by the IACUC of Dartmouth (Protocol number 00002014). They were kept in a temperature-controlled room under a 12 h light 12 h dark cycle with free access to commercial solid food and water. The mice were anesthetized using isoflurane prior to drawing blood and euthanized by an approved method while under anesthesia.
Shrestha A., Duwadi D., Jukosky J, & Fiering S.N. (2019). Cecropin-like antimicrobial peptide protects mice from lethal E.coli infection. PLoS ONE, 14(7), e0220344.
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5

Mouse Housing and Ethical Guidelines

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Female NZB/WF1 mice purchased from the Jackson Laboratory and female C57BL/6 mice purchased from Shanghai Laboratory Animal Center of the Chinese Academy of Sciences were used for this investigation. All mice were housed in a pathogen-free facility and were housed in clean-grade animal cabin with free access to standard laboratory water and food, and kept in a 12-h light/dark cycle with controlled humidity (60–80%) and temperature (22 ± 1 °C).
The animal experiment was carried out in strict accordance with the institutional ethical guidelines on animal care and was approved by the Institute Animal Care and Use Committee (IACUC) at the Shanghai Institute of Materia Medica, Chinese Academy of Sciences (IACUC protocol # 2012-06-ZJP-17 for NZB/WF1 mice, IACUC protocol # 2012-12-ZJP-18 for C57BL/6 mice).
He S., Liu X., Lin Z., Liu Y., Gu L., Zhou H., Tang W, & Zuo J. (2019). Reversible SAHH inhibitor protects against glomerulonephritis in lupus-prone mice by downregulating renal α-actinin-4 expression and stabilizing integrin-cytoskeleton linkage. Arthritis Research & Therapy, 21, 40.
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6

Age and Leucine Effects on Muscle Injury

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Female C57BL/6 mice were purchased from Jackson Laboratories and were housed in the University of Arkansas Central Laboratory Animal Facility as previously described (Washington et al., 2013 (link)). The mice were kept on a 12:12-h light-dark cycle with ad libitum access to normal rodent chow and water. Young (3 months) and aged (24 months) mice were randomly assigned to one of eight treatment groups: 1) young/no leucine/uninjured (n = 6); 2) young/no leucine/injured (n = 6); 3) young/leucine/uninjured (n = 6); 4) young/leucine/injured (n = 6); 5) aged/no leucine/uninjured (n = 6); 6) aged/no leucine/injured (n = 6); 7) aged/leucine/uninjured (n = 15); 8) aged/leucine/injured (n = 6). All procedures were approved by the University of Arkansas Institutional animal Care and Use Committee (IACUC).
Perry RA J.r., Brown L.A., Lee D.E., Brown J.L., Baum J.I., Greene N.P, & Washington T.A. (2016). Differential Effects of Leucine Supplementation in Young and Aged Mice at the Onset of Skeletal Muscle Regeneration. Mechanisms of ageing and development, 157, 7-16.
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7

Murine Immunology Protocol

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Female C57BL/6 mice and OT-I TCR transgenic mice were purchased from Jackson Laboratory and used at age 6–12 weeks. All experiments involving mice were carried out in accordance with an animal use protocol approved by the University of North Carolina Animal Care and Use Committee.
Kapadia C.H., Tian S., Perry J.L., Luft J.C, & DeSimone J.M. (2016). Reduction Sensitive PEG Hydrogels for Codelivery of Antigen and Adjuvant To Induce Potent CTLs. Molecular pharmaceutics, 13(10), 3381-3394.
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8

Murine STAT1-Deficient Immune Response

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Female C57BL/6 mice, 6–8 weeks old, were purchased from The Jackson Laboratory (Bar Harbor, ME). The mice were housed in our DLAR facilities and used under experimental protocols approved by the University of Pittsburgh Institutional Animal Care and Use Committee (IACUC). STAT1 KO mice were a kind gift from Dr. John Alcorn (University of Pittsburgh). All animal experiments were carried out in compliance with ARRIVE guidelines.
Sedlacek A.L., Kinner-Bibeau L.B., Wang Y., Mizes A.P, & Binder R.J. (2021). Tunable heat shock protein-mediated NK cell responses are orchestrated by STAT1 in Antigen Presenting Cells. Scientific Reports, 11, 16106.
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9

Tuberculosis Infection Model in Mice

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Female C57BL/6 mice (Jackson Laboratory) were infected by aerosol using an inhalation exposure system (Glas-Col) and early-log-phase M. tuberculosis cultures as single-cell suspensions in PBS to deliver 100 to 200 bacilli per mouse. Doxycycline containing food (2000 ppm, Research Diets) was given to mice starting at the indicated time-points. Serial dilutions of lungs and spleens homogenates were cultured on 7H10 plates containing ADNaCl to determine CFU at the indicated time points. The left lobe of each lung was fixed in 10% buffered formalin, further processed for histopathology and stained with hematoxilyn and eosin. We isolated and infected bone marrow-derived mouse macrophages as previously described [44] (link).
Puckett S., Trujillo C., Eoh H., Marrero J., Spencer J., Jackson M., Schnappinger D., Rhee K, & Ehrt S. (2014). Inactivation of Fructose-1,6-Bisphosphate Aldolase Prevents Optimal Co-catabolism of Glycolytic and Gluconeogenic Carbon Substrates in Mycobacterium tuberculosis. PLoS Pathogens, 10(5), e1004144.
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10

OT-I TCR-Tg Mouse Model Study

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Female C57Bl/6 mice were purchased from The Jackson Laboratory. CD45.1 OT-I TCR-Tg mice were provided by Dr. Vezys (University of Minnesota). All experimental protocols involving the use of mice were approved by the Institutional Animal Care and Use Committee at the University of Minnesota.
Fiege J.K., Stone I.A., Fay E.J., Markman M.W., Wijeyesinghe S., Macchietto M.G., Shen S., Masopust D, & Langlois R.A. (2019). THE IMPACT OF TCR SIGNAL STRENGTH ON RESIDENT MEMORY T CELL FORMATION DURING INFLUENZA VIRUS INFECTION. Journal of immunology (Baltimore, Md. : 1950), 203(4), 936-945.
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