Cd90 phycoerythrin

For immunophenotype characterization, 1 × 10⁵ PDLSCs were incubated with anti-human stem cell surface-labeled antibodies including CD73-phycoerythrin and CD90-phycoerythrin (BD Biosciences, USA), anti-hematopoietic marker CD34 antibodies (BD Biosciences, USA), and anti-endothelial cell marker CD31 antibodies (BD Biosciences, USA). All flow cytometry tests were performed on a FACSAria (BD Bioscience).

Upon trypsinization and washing in phosphate-buffered saline (PBS), phenotypic expression of cell surface markers was performed on BM-MSCs with flow cytometry using the following fluorochrome-conjugated anti-human antibodies: CD73-fluorescein isothiocyanate (FITC), CD90-phycoerythrin (PE), CD105-allophycocyanin (APC), CD34-Brilliant Violet (BV) 510 and CD45-peridinin chlorophyll protein (PerCP) Cy5.5 (BD Biosciences). Positively stained cell populations were identified by comparing with fluorescence-minus-one (FMO) controls. Acquisition was performed using a CytoFLEX flow cytometer (Beckman Coulter) and analysed using FlowJo V10.