High purity grade lithium hydroxide monohydrate, esterase, acetone, ethyl chloroacetate, ethyl acetate, dimethylformamide, sodium cholate, anhydrous potassium carbonate, hydrochloric acid, formic acid, acetic acid, amylase, thionyl chloride, ammonia solution, hexane, sodium bicarbonate, phosphate-buffered saline (PBS) buffer, sodium chloride, sodium sulfate, potassium iodide, dichloromethane, dialysis tubing, porcine pancreatin, silica gel, dimethyl sulfoxide, acetonitrile, porcine pepsin, 2-chloroacetamide, and 1,2-dibromoethane were purchased from Millipore Sigma (Burlington, MA, USA). The high purity grade apigenin, quercetin and luteolin were obtained from Indofine chemical company (Hillsborough, NJ, USA).
Dialysis tubing
Manufactured by Merck Group
Sourced in United States, United Kingdom
Dialysis tubing is a semi-permeable membrane used in various laboratory applications. It allows the passage of small molecules while retaining larger molecules and particles. The core function of dialysis tubing is to facilitate the separation and purification of macromolecules, such as proteins, nucleic acids, and other biomolecules, from their surrounding solutions.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (7 mentions)
Sodium azide, by Merck Group (4 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Dichloromethane, by Thermo Fisher Scientific (4 mentions)
Acetone, by Merck Group (3 mentions)
Phosphate buffered saline (pbs), by Merck Group (3 mentions)
Tween 20, by Merck Group (3 mentions)
Methanol, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
L glutamine, by Thermo Fisher Scientific (3 mentions)
Anhydrous potassium carbonate, by Merck Group (2 mentions)
Porcine pepsin, by Merck Group (2 mentions)
Sodium bicarbonate, by Merck Group (2 mentions)
Hexane, by Merck Group (2 mentions)
Silica gel, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Ammonia solution, by Merck Group (2 mentions)
Dimethylformamide, by Merck Group (2 mentions)
Thionyl chloride, by Merck Group (2 mentions)
56 protocols using dialysis tubing
1
Extraction and Derivatization of Flavonoids
2
Flavonoids Synthesis and Characterization
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High purity flavonoids, quercetin, apigenin, luteolin, fisetin, kaempferol (Indofine Chemical Company, Hillsborough, NJ, USA), and ethyl chloroacetate, dimethylformamide, anhydrous potassium carbonate, lithium hydroxide, hydrochloric acid, ethyl acetate, thionyl chloride, acetone, ammonia solution, hexane, silica gel, sodium bicarbonate, sodium chloride, sodium sulfate, sodium cholate, porcine pepsin, dialysis tubing, amylase, esterase, porcine pancreatin, dimethyl sulfoxide, and phosphate-buffered saline were purchased from Millipore Sigma (Burlington, MA, USA).
3
In vitro 5-FU Release from Cubosomes
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In vitro release of 5-FU from cubosomes was evaluated using a dynamic dialysis method27 . The release rate of drug was determined after separation of free drug from drug-loaded cubosomes by placing the cubosomal dispersion in dialysis tubing (10,000 MWCO, Millipore, Boston, USA) and exhaustively dialyzed for 15 min for several times, each time against 100 mL of phosphate buffer (pH 7.4)28 . The dialysis of free 5-FU was completed after 1 h after which no further drug could be detected in the solution. The dialyzed suspension containing 5-FU-loaded cubosomes (equivalent to 1 mg drug) or plain drug aqueous solution was sealed in a dialysis bag (10,000 MWCO, Millipore, Boston, USA). The dialysis bag was then immersed in 100 mL of phosphate buffer (pH 7.4) thermostatically maintained at 37±0.5 °C and magnetically stirred at 50 rpm. The samples (3 mL) were withdrawn at various time intervals and analyzed by a UV spectrophotometer at 266 nm. Volumes lost by sample withdrawal were replaced with fresh medium. The experiments were conducted in triplicate.
4
Synthesis and Characterization of PAMAM Dendrimers
5
Detecting Carbonyl Modifications in Proteins
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3-DG, arginine, lysine, calf thymus H1 histone protein, sodium azide; 9,10-phenanthrenequinone, 2,4-dinitrophenyl hydrazine (DNPH); dialysis tubing, and other reagents/chemicals were obtained from Sigma Chemical Company (St. Louis, MO, USA). Nitroblue tetrazolium (NBT) was from Sisco Research Laboratories (India).
6
Characterizing β-Glucosidase Stability Profiles
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Crude enzyme sample used in these experiments were desalted against distilled water at 1:1,000 ratio (v/v) using dialysis tubing of molecular weight cut-off 12,000 Da (Sigma-Aldrich, St. Louis, MO, USA), and then concentrated against sucrose for 4 h. The stability of β-glucosidase against urea, maltose, and fructose, selected salts (ZnSO 4 , FeCl 2 , KCl, MgCl 2 , MnCl 2 , Cd(NO 3 ) 2 , Cr(NO 3 ) 2 , SO 4 Cu), chelators (ethylenediaminetetraacetic acid (EDTA) and Azida), and surfactants (sodium dodecyl sulfate (SDS) and cetyltrimethylammonium bromide (CTAB)), was tested at 1 and 10 mM. For this, the culture fluid was preincubated with the individual additives at 30ºC. Samples were collected after 30 min for enzyme activity determination at standard assay conditions. Control with a preincubation in the absence of any additive was recorded as 100% activity. In addition, the effect of organic solvents on β-glucosidase stability was assayed. Culture filtrate was incubated with methanol (50% (v/v)), acetic acid (50% (v/v)), or ethanol (25, 50, and 75% (v/v)) at 30ºC. Samples were collected at selected times and residual activities were determined.
7
In Vitro Drug Release Kinetics
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The freeze-dried Dox-Rif-PLGA@CdTe were accurately weighed and placed inside a dialysis bag (molecular weight 12,000, specifically, Dialysis tubing from Sigma Chem. Co., Missouri, USA). This dialysis bag containing the nanoparticles was immersed in a 40 ml release solution consisting of a PBS buffer with a pH of 7.4. The setup was then subjected to magnetic stirring at 100 rpm and kept at 37 °C. About 1 ml of samples was collected from the release medium at predetermined intervals, and a spectrophotometer was used to determine the doxycycline and rifampicin content. A similar procedure was performed to compare the results obtained from free doxycycline and rifampicin, where free doxycycline and rifampicin were placed inside dialysis bags and immersed in the same release medium. Repeated samples were drawn from the medium at these same intervals for analysis. An equal volume of fresh medium was replenished after each sample was taken from the medium [36 ].
8
Synthesis and Characterization of mPEG-Peptide Conjugates
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H-lys(Z)-OH ≥ 99.0% (NT), triphosgene, tetrahydrofuran anhydrous (stabilized with BHT), N-hexane, ethyl acetate, and methoxy-poly(ethylene glycol)-NH2 (mPEG-NH2, MW 2000) were purchased from Laysan Bio, N,N-dimethylformamide (DMF) anhydrous, diethyl ether, trifluoroacetic acid, HBr/acetic acid solution (33%), succinic anhydride ≥ 99% (GC), dialysis tubing (molecular weight cutoff; MWCO 3.5 K), and Vivaspin 500 (300 KDa) were purchased from Sigma-Aldrich (Burlington, MA, USA), OVA–FITC was purchased from Thermo Fisher Scientific (San Jose, CA, USA), Neogreen was obtained from NEO Science (Dubai, United Arab Emirates), and CleanCap Enhanced Green Fluorescent Protein (EGFP) mRNA (5moU) was obtained from TriLink (San Diego, CA, USA).
9
Purification and Analysis of Antithrombin Dimers
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Antithrombin disulphide‐linked dimers from plasma of patients with c.1154‐14G>A or the c.334C>T mutations were purified as described before.12 Briefly, citrated plasma of patients were subjected to heparin affinity chromatography on HiTrap Heparin columns (GE Healthcare), followed by ion exchange chromatography on HiTrap Q columns (GE Healthcare) and a gel filtration. All procedures were done in an ÄKTA Purifier (GE Healthcare). Finally, proteins eluted were desalted through a dialysis tubing (Sigma‐Aldrich) and stored at −70°C, prior to analysis. After electrophoretic analysis (SDS‐PAGE), bands corresponding to dimers purified from patients with c.1154‐14G>A mutation and wild type antithrombin, were subjected to in‐gel digestion with 200 ng of sequencing grade modified Trypsin (Promega, Madrid, Spain) in 50 mmol L−1 ammonium bicarbonate for 16 hour at 37°C, after a denaturation step with DTT (10 mmol L−1, 30 minute, 40°C) and an alkylation step with Iodoacetamide (25 mmol L−1, 30 minute, room temperature). The resulting peptides were extracted with 1% formic acid, 50% acetonitrile and evaporated to dryness prior to LC‐MSMS analysis.
10
Methacrylated Gelatin Synthesis
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Gelatin (type A from porcine skin, bloom strength 300), Methacrylic Anhydride (MA) (contains 2000 ppm topanol A as inhibitor, 94%), and dialysis tubing (MWCO 12,400) were purchased from Sigma. All reactions were carried out at 50 °C, 10 grams of Gelatin were dissolved in 80 mL deionized water. Sodium hydroxide 1.2 M was used to adjust the pH of clear Gelatin solution to 9, then 6 mL of MA were added dropwise into the hot Gelatin solution (at 50 °C) under vigorous stirring. After 3 hours of reaction, 200 mL of deionized water was added to stop the reaction, then all of the solution was put into dialysis tubing dipped in plenty of hot deionized water (~5 L) for 5 days. The water was changed every day. After dialysis, the gel solution was lyophilized for 5 days and stored in –80 °C.
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