Caseviewer 2

Manufactured by 3DHISTECH

Sourced in Hungary, Japan, China

CaseViewer 2.4 is a digital pathology software application developed by 3DHISTECH. The software is designed to view and analyze digital slide images captured from whole slide imaging scanners.

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Lab products found in correlation

Pannoramic midi, by 3DHISTECH (12 mentions) Image pro plus 6, by Media Cybernetics (10 mentions) Pannoramic desk midi 250 1000, by 3DHISTECH (8 mentions) Pannoramic 250 flash 3, by 3DHISTECH (7 mentions) Pannoramic scan, by 3DHISTECH (6 mentions) Pannoramic 250 flash, by 3DHISTECH (6 mentions) Pannoramic 250, by 3DHISTECH (5 mentions) Pannoramic midi scanner, by 3DHISTECH (4 mentions) Pannoramic scan 2, by 3DHISTECH (4 mentions) Mirax scanner, by 3DHISTECH (3 mentions) Halo v3, by Indica Labs (3 mentions) Pannoramic digital slide scanner, by 3DHISTECH (3 mentions) Pannoramic midi slide scanner, by 3DHISTECH (2 mentions) Formalin solution, by Merck Group (2 mentions) Panoramic scan 2 scanner, by 3DHISTECH (2 mentions) Pannoramic midi 2, by 3DHISTECH (2 mentions) Panoramic 250 flash 3, by 3DHISTECH (2 mentions) Pannoramic 1000, by 3DHISTECH (2 mentions) Mirax midi, by 3DHISTECH (2 mentions) Pannoramic desk, by 3DHISTECH (2 mentions)

Close spelling variants of this product

CaseViewer (162 citations) CaseViewer v.2.3 (11 citations) Caseviewer version 2.4 (10 citations) CaseViewer ver. 2.1 (6 citations) CaseViewer Software 2.4 (5 citations) Caseviewer 2.0 (Panoramic 250/MIDI, (2 citations) CaseViewer software (version 2.3) (2 citations)

199 protocols using caseviewer 2

Intestinal Mucosa Damage Assessment

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Tissues were fixed in 10% neutral buffered formalin and embedded in paraffin. They were cut in 3 μm thick sections and stained with hematoxylin and eosin. Slides were digitized with Mirax scanner and photographs were taken with CaseViewer 2.4 software (3DHISTECH Ltd., Budapest, Hungary). Intestinal mucosa damage was evaluated blindly by two individuals. The degree of injury was determined using the scoring system described by Park et al. [8 (link)]. (See Table 1). A minimum of three fields randomly selected from four quadrants of each intestinal sample were evaluated.
Morphometric analysis of total mucosa thickness and villous depth was analyzed using the CaseViewer 2.4 software (3DHISTECH Ltd.). Total mucosa thickness was assessed by measuring the distance between the villus tip to the lamina-muscularis mucosae in at least four axially oriented villi in four quadrants. Crypt depth was determined in at least a total of five axially oriented, open, non-destroyed crypts from three quadrants.

Caleb I., Kasza B., Erlitz L., Semjén D., Hardi P., Makszin L., Rendeki S., Takács I., Nagy T, & Jancsó G. (2022). The Effects of Rapamycin on the Intestinal Graft in a Rat Model of Cold Ischemia Perfusion and Preservation. Metabolites, 12(9), 794.

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Intestinal Tissue Histopathological Analysis

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Tissues were fixed in 10% neutral buffered formalin and embedded in paraffin. They were cut in 3-micrometer-thick sections and stained with hematoxylin and eosin. The slides were digitized with a Mirax scanner, and photographs were taken with CaseViewer 2.4 software (3DHISTECH Ltd. Budapest, Hungary). Intestinal mucosa damage was evaluated blindly by two individuals. The degree of injury was determined using the Park/Chiu system described by Park et al. [8 (link)]. A minimum of three fields randomly selected from four quadrants of each intestinal sample was evaluated.
Morphometric analysis of total mucosa thickness and villous depth was analyzed using CaseViewer 2.4 software (3DHISTECH Ltd. Budapest, Hungary). Total mucosa thickness was assessed by measuring the distance between the villus tip to the lamina-muscularis mucosae in at least four axially oriented villi in four quadrants. Crypt depth was determined in at least a total of five axially oriented, open, non-destroyed crypts from three quadrants.

Caleb I., Erlitz L., Telek V., Vecsernyés M., Sétáló G J.r., Hardi P., Takács I., Jancsó G, & Nagy T. (2021). Characterizing Autophagy in the Cold Ischemic Injury of Small Bowel Grafts: Evidence from Rat Jejunum. Metabolites, 11(6), 396.

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Histological Analysis of Intestinal Tissues

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A piece (about 3 mm in length) of fresh duodenum, jejunum, and ileum tissues from midden section of each intestinal segment were cut, respectively, and fixed with 4% paraformaldehyde solution for 1 wk. These fixed tissues were gradual dewaxing and dehydration with graded xylene and ethanol, and then embedded in paraffin. Afterward, the embedded tissues were cut into the sections of 5-μm thickness by a microtome. The sections were stained using hematoxylin and eosin dye liquor, and then these prepared sections were observed and analyzed by an optical microscope (Eclipse E100, Nikon, Japan) (Wei et al., 2023 ). The histopathologic examination of stained sections was performed under a 50X of amplification with a visual field of 20 μm using the CaseViewer 2.4 (3DHISTECH, Hungary) software, and corresponding pictures were also taken. Additionally, the villus length and crypt depth of stained sections from each intestinal tissue were measured at a 5X of amplification with a visual field of 200 μm by the CaseViewer 2.4 (3DHISTECH, Hungary) software, and the ratio of villus length and crypt depth was also calculated.

Bi Y., Wei H., Chai Y., Wang H., Xue Q, & Li J. (2024). Intermittent mild cold acclimation ameliorates intestinal inflammation and immune dysfunction in acute cold-stressed broilers by regulating the TLR4/MyD88/NF-κB pathway. Poultry Science, 103(5), 103637.

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Analyzing Breast Tumor Tissue Sections

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Images of human breast tumor tissue sections were analyzed with the CaseViewer 2.0 software (3DHistech, Budapest, Hungary). Different histological subregions, including histologically normal mammary gland epithelium, carcinoma in situ, local invasion into the fibrous or adipose tissue, or intravascular cells were independently scored in a blinded manner by two researchers for high, intermediate or absent fluorescence intensity of p120-1, p120-3 and E-cadherin (Figure S6). To account for the non-Gaussian distribution of data, a non-parametric Kruskal–Wallis test was used with a post-hoc Dunn’s test for multiple comparison.

Venhuizen J.H., Span P.N., van den Dries K., Sommer S., Friedl P, & Zegers M.M. (2019). P120 Catenin Isoforms Differentially Associate with Breast Cancer Invasion and Metastasis. Cancers, 11(10), 1459.

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Brain Tissue Preparation and Immunostaining

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Each mouse was dissected immediately after transcardial perfusion with 4% paraformaldehyde (PFA). The brain was isolated and post-fixed in 4% PFA overnight. Fixed tissues were serially sectioned (4 μm) after paraffin embedding. The sections were dewaxed with gradient xylene and anhydrous ethanol, antigen repaired with high temperature, blocked with 10% goat or donkey serum, and immunostained with specific antibodies. After immunostaining, the sections were panoramically scanned with a scanning microscope and were analyzed with CaseViewer 2.0 software (3D HISTECH, Budapest, Hungary). Some representative images were obtained through a fluorescence microscope (Olympus).

Gao F., Liu A., Qi X., Wang M., Chen X., Wei S., Gao S., Sun Y., Sun P., Li X., Sun W., Li J, & Liu Q. (2022). Ppp4r3a deficiency leads to depression-like behaviors in mice by modulating the synthesis of synaptic proteins. Disease Models & Mechanisms, 15(6), dmm049374.

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Decalcification and Histological Analysis of Transgenic Mouse Tissue

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Formalin‐fixed Hesx1^Cre/+;Ctnnb1^lox(ex3)/+ mouse tissue imaged by µ‐CT was decalcified in 1% formic formaldehyde for 48 h before embedding in paraffin blocks. A further 5 surface decalcifications of 2 h each were performed prior to 5 µm sections being cut in the axial or sagittal plane, matched to the imaging plane. Sections were then stained with hematoxylin and eosin (H&E).
The brains from patient‐derived xenograft‐bearing mice were formalin‐fixed and processed following µCT imaging, and H&E staining of 3 µm axial sections was performed as previously described 26.
Slides were scanned using either Nanozoomer (Hamamatsu Photonics, Welwyn Garden City) or Pannoramic MIDI (3D‐Histech, Sysmex Europe) and processed using CaseViewer 2.0 software (3D‐Histech, Budapest, Hungary).

Boult J.K., Apps J.R., Hölsken A., Hutchinson J.C., Carreno G., Danielson L.S., Smith L.M., Bäuerle T., Buslei R., Buchfelder M., Virasami A.K., Koers A., Arthurs O.J., Jacques T.S., Chesler L., Martinez‐Barbera J.P, & Robinson S.P. (2017). Preclinical transgenic and patient‐derived xenograft models recapitulate the radiological features of human adamantinomatous craniopharyngioma. Brain Pathology, 28(4), 475-483.

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Lectin Histochemistry of Pig Uterus

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The lectin‐histochemical study was performed on pig uterine cross sections. Slides were incubated with either fluorescein isothiocyanate (FITC) labelled lectin Sambucus nigra (SNA, 1:150, FL‐1391; Vector Labs) or biotin‐conjugated lectin Maackia amurensis (MAL‐II, 1:150, B‐1265; Vector Labs) at 4°C overnight. The biotin‐conjugated lectin was subsequently detected with streptavidin‐FITC conjugate (1:100, SA‐5001; Vector Labs). Nuclei were stained with DAPI (G1012; Servicebio) and sealed with anti‐fluorescence quenching tablets. All slides were scanned by a Pannoramic Midi slide scanner (3D HISTECH), and the image analysis was carried out by using CaseViewer 2.0 software (3D HISTECH).

Han K., Wang F., Yue Y., Tan X., Tian M., Miao Y., Zhao S., Dong W, & Yu M. (2021). Glycomics reveal that ST6GAL1‐mediated sialylation regulates uterine lumen closure during implantation. Cell Proliferation, 55(1), e13169.

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Quantifying Tumor-Infiltrating Lymphocytes by Immunohistochemistry

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To evaluate the impact of TILs, we determined the immunoscores by immunohistochemical staining for CD3 and CD8. We scanned all the immunohistochemically stained slides for CD3 and CD8 using a Pannoramic MIDI II slide scanner (3DHISTECH, Budapest, Hungary). We captured images from two regions—the core of the tumor (CT) and invasive margin (IM)—using the CaseViewer 2.0 software (3DHISTECH). CD3- and CD8-immunoreactive lymphocytes were identified from the captured images using NIH Image Analysis software (version 1.6.0; National Institutes of Health, Bethesda, Maryland, USA) after setting a consistent intensity threshold. The CD3- and CD8-immunoreactive lymphocytes were expressed as pixels in each region. Using the cut-off (median value), the pixel value of each case was classified to high (score 1) and low (score 0). Immunoscores were defined as the sum of the scores of two regions and are divided into high (score 3–4) and low (score 0–2) scores [21 (link)].

Oh I.H., Pyo J.S, & Son B.K. (2021). Prognostic Impact of YKL-40 Immunohistochemical Expression in Patients with Colorectal Cancer. Current Oncology, 28(4), 3139-3149.

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Histological Analysis of Colon Injury

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One centimeter of the distal colon was fixed in 10% formaldehyde followed by paraffin embedding. The colon tissue was then sectioned into 5μm thick sections dewaxed in xylene, dehydrated with gradient ethanol, and stained with hematoxylin for five minutes and with eosin for one minute. The colon sections were then observed under a microscope (Olympus, BX53, Japan). The villus length (μm) of the HE-stained sections was determined using Case Viewer 2.0 software (3DHISTECH Ltd.). Villus length was defined as the distance from the top of the villi to the muscularis of the intestinal wall. The gross morphological score for colon injury was evaluated based on the parameters shown in Table 2 (1 (link)). The samples were evaluated by experienced pathologists who were blinded to each other.

Zhou H.C., Yu W.W., Yan X.Y., Liang X.Q., Ma X.F., Long J.P., Du X.Y., Mao H.Y, & Liu H.B. (2022). Lactate-driven macrophage polarization in the inflammatory microenvironment alleviates intestinal inflammation. Frontiers in Immunology, 13, 1013686.

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Histological Assessment of Wound Tissue

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Wound tissue explants at days 1, 3 and 5 were embedded in OCT compound, snap-frozen in liquid nitrogen and stored at −80 °C. Ten micrometer sections were prepared with the use of a cryostat. Sections were stained for nuclei with haematoxylin (Sigma-Aldrich, Saint Louis, USA) and cytoplasm counterstained with eosin (Sigma-Aldrich, Saint Louis, USA) in a Varistain™ 24-4 Automatic Slide Stainer (ThermoFisher Scientific, Basingstoke, UK). This involved fixation of sections for 2 min in 70% industrial methylated spirits (IMS), followed by a 2 min wash in water. Sections were stained for 2 min in haematoxylin Gills 2, and then in water. Tissue sections were then washed in 5% acetic acid and dehydrated in graded IMS/ethanol (70%, 90% and 100%). Sections were counterstained with alcoholic eosin Y solution for 90 s followed by three 100% ethanol washes. Finally, slides were clarified with xylene and then mounted in mounting media. Images were acquired using a 20x/0.80 Plan Apo objective using the Pannoramic 250 Flash II slide scanner (3D Histech Ltd, Budapest, Hungary). Images were then processed and analysed using CaseViewer 2.0 software (3D Histech Ltd, Budapest, Hungary).

Ashrafi M., Novak-Frazer L., Bates M., Baguneid M., Alonso-Rasgado T., Xia G., Rautemaa-Richardson R, & Bayat A. (2018). Validation of biofilm formation on human skin wound models and demonstration of clinically translatable bacteria-specific volatile signatures. Scientific Reports, 8, 9431.

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