At the end of the experiment (hour 120), all surviving tadpoles were evaluated for histopathological changes by fixing them in Bouin's solution for 8 h and then preserving them in 70 % ethyl alcohol (Sigma-Aldrich) until the preparation day. On the preparation day, the tadpoles were passed through 80 %, 90 %, 96 %, and absolute ethyl alcohol series and later through xylene (Sigma-Aldrich) and paraffin series to be embedded in paraffin blocks. We made 8-µmthick cross sections and stained them with haematoxylin & eosin (H&E; Sigma-Aldrich) for histopathological examination with an Olympus CX21 light microscope. We also took photographs with a camera adapted to the Olympus BX51 light microscope and analysed them with Olympus Analysis LS software.
Ethyl alcohol
Manufactured by Merck Group
Sourced in United States, Germany, Sao Tome and Principe, Ireland, Brazil, India
Ethyl alcohol, also known as ethanol, is a clear, colorless liquid that is commonly used as a solvent and disinfectant in laboratory settings. It is a versatile compound with a wide range of applications in scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Hydrochloric acid (hcl), by Merck Group (24 mentions)
Sodium hydroxide (naoh), by Merck Group (22 mentions)
Acetonitrile, by Merck Group (21 mentions)
Methyl alcohol, by Merck Group (15 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (14 mentions)
Chloroform, by Merck Group (13 mentions)
Acetone, by Merck Group (12 mentions)
Acetic acid, by Merck Group (12 mentions)
Sodium chloride (nacl), by Merck Group (11 mentions)
Methanol, by Merck Group (11 mentions)
Formic acid, by Merck Group (9 mentions)
Gallic acid, by Merck Group (9 mentions)
Potassium chloride (kcl), by Merck Group (9 mentions)
Hydrogen peroxide, by Merck Group (9 mentions)
N n dimethylformamide (dmf), by Merck Group (8 mentions)
Phosphoric acid, by Merck Group (8 mentions)
Sulfuric acid, by Merck Group (8 mentions)
Isopropyl alcohol, by Merck Group (7 mentions)
Citric acid, by Merck Group (7 mentions)
Milli q system, by Merck Group (7 mentions)
214 protocols using ethyl alcohol
1
Histopathological Examination of Tadpoles
2
Gut Ultrastructure and Elemental Analysis
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Three guts
were separated from the third instar larva of each concentration and
stored at 4 °C in 4% paraformaldehyde (PFA) (158127, Sigma-Aldrich,
Merck, Germany). To eliminate the extra PFA (158127, Sigma-Aldrich,
Merck, Germany), the guts were rinsed with PBS. The guts were dehydrated
using a graded serial dehydration method that involved increasing
the percentage of ethyl alcohol (1.00983, Sigma-Aldrich, Merck, Germany).
The concentrations of the ethyl alcohol used were 30, 50, 70, 90,
and 100%. The desiccated guts were mounted on a slide containing carbon
tape and then punctured in the midgut region to expose the gut lumen.
Scanning electron microscopy (SEM) (JEOL JSM-6480LV) analysis followed
by coating the samples with platinum. The quantity of manganese and
iron was determined by energy-dispersive spectroscopy (EDS) analysis.40 (link),41 (link)
were separated from the third instar larva of each concentration and
stored at 4 °C in 4% paraformaldehyde (PFA) (158127, Sigma-Aldrich,
Merck, Germany). To eliminate the extra PFA (158127, Sigma-Aldrich,
Merck, Germany), the guts were rinsed with PBS. The guts were dehydrated
using a graded serial dehydration method that involved increasing
the percentage of ethyl alcohol (1.00983, Sigma-Aldrich, Merck, Germany).
The concentrations of the ethyl alcohol used were 30, 50, 70, 90,
and 100%. The desiccated guts were mounted on a slide containing carbon
tape and then punctured in the midgut region to expose the gut lumen.
Scanning electron microscopy (SEM) (JEOL JSM-6480LV) analysis followed
by coating the samples with platinum. The quantity of manganese and
iron was determined by energy-dispersive spectroscopy (EDS) analysis.40 (link),41 (link)
3
Extraction and Analysis of Seed Oils
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Virgin oil of hemp seed and evening primrose were extracted from the commercial seeds with a cold pressing standardized method, a mechanical extraction process, at Giah Essence Agro-Industry & Phytopharm Company, Gorgan, Golestan Province, I.R. Iran. The analysis of the extracted oils was determined by gas chromatography (Table 1 ). RAPA in powder form (Santa Cruz Biotechnology, United States) was dissolved in 1 mL ethyl alcohol (Merck, Germany) and diluted with distilled water. The RAPA solution was kept at 4 °C in the dark according to the manufacturer’s instruction. The control solution contains only 1% ethyl alcohol (Merck, Germany) and diluted with distilled water.
4
Extraction and Analysis of Industrial Seed Oils
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Pure HSO and EPO were isolated from the tops of fresh varieties of industrial seeds by the cold pressing standardized method, a mechanical extraction process, in Giah Essence Agro-Industry & Phytopharm Company, Gorgan, Golestan Province, I.R. Iran. The analysis of the fatty acids of the extracted oils was determined by gas chromatography (Table 1 ). RAPA in powder form (Santa Cruz Biotechnology, United States) was dissolved in 1 mL of ethyl alcohol (Merck, Germany) 1 mL of ethyl alcohol (Merck, Germany) which was diluted with distilled water. The RAPA solution was stored at 4 °C in the dark according to the manufacturer's instruction.
5
Antioxidant Activity Screening Protocol
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2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ethyl acetate, and ethyl alcohol were obtained from MilliporeSigma (Burlington, MA, USA) and Thermo Fisher Scientific (Waltham, MA, USA), respectively. Potassium persulfate was procured from PanReac AppliChem (Barcelona, Spain). H was purchased from Macron Fine Chemicals (Radnor, PA, USA). All reagents and chemicals used were of analytical grade.
6
Antimicrobial Compound Screening Protocol
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Chemicals and reagents used in this work were purchased from commercial suppliers. FeCl3, 8-hydroxyquinoline, ethyl alcohol, chloroform, HNO3, HCl, DMSO, 2,2′ bipyridine (bipy) and thiourea (TU) were obtained from MilliporeSigma, while the bacterial and mammalian cells were all purchased from the American Type Culture Collection (Manassas, VA, USA). Bioluminescent Staphylococcus aureus (Xen36) was purchased from PerkinElmer. Ciprofloxacin (≥98%), imipenem (≥98%), mupirocin (≥98%), and fusidate (≥98%) were purchased from Sigma-Aldrich (St. Louis, MS, USA). All cell culture medium and the associated supplies were obtained from Fisher Scientific (Hampton, NH, USA).
7
Polyamide Thin-Film NF Membrane Fabrication
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Ammonium hydroxide solution (ACS grade, 28.0–30.0% NH3 basis), ethyl alcohol (anhydrous, 200 proof), N,N-dimethylformamide (DMF, 99.8%), succinic anhydride (99%), and tetraethyl orthosilicate (TEOS, 99.99%) were purchased from MilliporeSigma (St. Louis, MO, USA) and used as received. Polyamide thin-film NF membrane sheets (GE HL) were purchased from Sterlitech Corp. (Kent, WA, USA). The silicon line-and-groove stamps were purchased from Digi-Key Electronics (Thief River Falls, MN, USA). Deionized (DI) water was used to prepare the solutions.
8
Synthesis and Characterization of KLS-13019
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The synthesis of KLS-13019 has been described previously in detail (Kinney et al., 2016) . Verification of the structural identity for KLS-13019 was determined by 1 H NMR, LC/UV and LC/MS. The purity of KLS-13019 was 98.6% as determined by LC/MS. CBD and morphine sulfate were obtained through the National Institute on Drug Abuse (NIDA) Drug Supply Program (Bethesda, MD, USA). The purity of CBD was certified to be >99% pure and was confirmed by a thirdparty certificate of analysis as well. CBD and KLS-13019 were dissolved in a vehicle of 1:1:18 ethyl alcohol:cremophor:saline (v/v).
ethyl alcohol, Cremophor EL ® , and 9% sodium chloride solution were purchased from Millipore Sigma (St. Louis, MO, USA). Morphine was dissolved in 0.9% saline. Paclitaxel was obtained as a 6 mgÁml -1 concentration stock solution (Hospira, Inc., Lake Forest, IL, USA) and then further diluted in saline. As CBD is a natural product extracted from Cannabis sativa, these studies are reported in compliance with the recommendations made by the British Journal of Pharmacology (Izzo et al., 2020) .
ethyl alcohol, Cremophor EL ® , and 9% sodium chloride solution were purchased from Millipore Sigma (St. Louis, MO, USA). Morphine was dissolved in 0.9% saline. Paclitaxel was obtained as a 6 mgÁml -1 concentration stock solution (Hospira, Inc., Lake Forest, IL, USA) and then further diluted in saline. As CBD is a natural product extracted from Cannabis sativa, these studies are reported in compliance with the recommendations made by the British Journal of Pharmacology (Izzo et al., 2020) .
9
Polymer Solutions for Nanofiber Fabrication
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PAN polymer (Mw = 150 000 g/mol, Aldrich) was dissolved in DMF (99.8%, Aldrich) to produce 8, 9, and 10 wt% solutions. PMMA (Mw = 120 000 g/mol, Aldrich), PS (Mw = 280 000 g/mol), and TPU (Neothane, DongSung corp.) were dissolved in THF as solvent to produce 6, 8, and 10 wt% polymeric solutions, respectively. And thus, PEO (Mv = 200 000, powder, Aldrich) and PVP (Mw = 360 000, Aldrich) were dissolved in a solution including 75 v% of ethyl alcohol (Aldrich) and 25 v% of distilled water for production of 10, 12.5, 15, 17.5, 22.5, and 25 wt% solutions respectively. To obtain PVDF nano fiber, PVDF (Mw ~275 000 by GPC, Aldrich) was dissolved as 24 wt% in 50% of acetone and 50% of DMF mixture.
10
Synthesis of Phosphine Cobalt Complexes
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Tert-butyl(diphenyl)phosphine (PtBuPh2) (Aldrich, St. Louis, MO, USA, 97%), (S)-(+)neomenthyldiphenylphosphine [(S)-NMDPP] (Strem Chemicals, Newburyport, MA, USA, 98%), anhydrous cobalt dichloride (Aldrich, 99.9%), and MAO (Aldrich, 10 wt.% solution in toluene) were used as received. Ethyl alcohol (Aldrich, ≥99.8%) was degassed under vacuum, then by bubbling dry dinitrogen and kept over molecular sieves; pentane (Aldrich, ≥99.5%) was refluxed over Na/K alloy for ca. 8 h, then distilled and stored over molecular sieves under dry dinitrogen; toluene (Aldrich, ≥99.5% pure) was refluxed over Na for ca. 8h, then distilled and stored over molecular sieves under dry dinitrogen. 1,3-Butadiene (Aldrich, ≥99.5%) was evaporated from the container prior to each run, dried by passing through a column packed with molecular sieves and condensed into the reactor which had been precooled to −20 °C. All the phosphine cobalt complexes were synthesized as indicated below, following a general procedure already reported in the literature [23 (link)].
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